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A flaxseed meal protein extraction process

A technology for extracting linseed meal and protein, which is applied in the field of linseed meal protein extraction technology, can solve problems such as bad flavor, affecting the development and application of linseed protein resources, and poor utilization of plant protein resources, and achieves the effect of simple process steps

Inactive Publication Date: 2019-01-15
QINGHAI SCI TECH INFORMATION RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The oil exists in the cell, the protein mainly exists in the aleurone grains, and the rest exists as the structure of the cell itself, and it is difficult to separate the shell from the meat
[0003] At present, my country's linseed processing industry is still in its infancy, and its products are limited to linseed oil. In the traditional process, high-temperature steaming and frying will denature the protein in linseed and the unsaturated fatty acids in linseed, resulting in bad flavor. Some of them are limitedly added to feed or farmland fertilizer, so that valuable plant protein resources are not well utilized, which affects the development of flax protein resources and their application in the food industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A linseed meal protein extraction process is carried out sequentially according to the following steps:

[0018] Step 1: Put the flaxseed in a grinder for crushing, sieve to remove part of the flaxseed hulls to obtain flaxseed powder, put the flaxseed powder into a mixed solvent for oil extraction, and filter to obtain flaxseed meal; The mixed solvent is a mixed solution of ethanol-ammonia-n-hexane.

[0019] Step 2: adding an enzyme inhibitor to the obtained linseed meal; the enzyme inhibitor is a compound plant hydrolytic enzyme.

[0020] Step 3: putting the linseed meal added with the enzyme inhibitor into a water bath kept at 50°C for heating, stirring, and filtering;

[0021] Step 4: Add distilled water to the filtered material, the ratio of the material to distilled water is 1:14, stir; after stirring evenly, add sodium hydroxide solution, adjust the pH to 8, and perform ultrasonic treatment; the temperature of ultrasonic treatment is 53°C

[0022] Step 5: Add c...

Embodiment 2

[0024] A linseed meal protein extraction process is carried out sequentially according to the following steps:

[0025] Step 1: Put the flaxseed in a grinder for crushing, sieve to remove part of the flaxseed hulls to obtain flaxseed powder, put the flaxseed powder into a mixed solvent for oil extraction, and filter to obtain flaxseed meal; The mixed solvent is a mixed solution of ethanol-ammonia-n-hexane.

[0026] Step 2: adding an enzyme inhibitor to the obtained linseed meal; the enzyme inhibitor is a compound plant hydrolytic enzyme.

[0027] Step 3: putting the linseed meal added with the enzyme inhibitor into a water bath kept at 50°C for heating, stirring, and filtering;

[0028] Step 4: Add distilled water to the filtered material, the ratio of the material to distilled water is 1:25, stir; after stirring evenly, add sodium hydroxide solution, adjust the pH to 9, and perform ultrasonic treatment; the temperature of ultrasonic treatment is 53°C

[0029] Step 5: Add c...

Embodiment 3

[0031] A linseed meal protein extraction process is carried out sequentially according to the following steps:

[0032] Step 1: Put the flaxseed in a grinder for crushing, sieve to remove part of the flaxseed hulls to obtain flaxseed powder, put the flaxseed powder into a mixed solvent for oil extraction, and filter to obtain flaxseed meal; The mixed solvent is a mixed solution of ethanol-ammonia-n-hexane.

[0033] Step 2: adding an enzyme inhibitor to the obtained linseed meal; the enzyme inhibitor is a compound plant hydrolytic enzyme.

[0034] Step 3: putting the linseed meal added with the enzyme inhibitor into a water bath kept at 50°C for heating, stirring, and filtering;

[0035] Step 4: Add distilled water to the filtered material, the ratio of the material to distilled water is 1:35, stir; add sodium hydroxide solution after stirring evenly, adjust the pH to 10, and perform ultrasonic treatment; the temperature of ultrasonic treatment is 53°C

[0036] Step 5: Add c...

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PUM

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Abstract

The invention belongs to the technical field of food processing, in particular to a flaxseed meal protein extraction process. the process includes heating the flaxseed meal into which an enzyme inhibitor is added in a water bath pot maintained at a certain temperature, stirring and filtering; adding a certain proportion of distilled water into the filtered material and stirring; adding sodium hydroxide solution after stirring uniformly, adjusting ph to a certain value, and carrying out ultrasonic treatment; adding cross-linked polyvinylpyrrolidone to the supernatant after ultrasonic treatment,and then adjusting the extract to isoelectric point by hydrochloric acid, and collecting the precipitate after ultrasonic centrifugation. In accordance with the process, through the proportion of ethanol-ammonia-n-hexane mixture, and the ratio of a material and distilled water, the content of crude protein in flaxseed meal is increased and the content of protein in flaxseed meal is further increased to over 92%.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a flaxseed meal protein extraction process. Background technique [0002] Flaxseed is the dry mature seed of Linum usitatissimum L., a plant of the genus Flax in the family Linaceae. It is also known as flaxseed and linseed. for wild. Flaxseed is an important plant resource for both medicine and food. It is recorded in the "Chinese Pharmacopoeia". Modern pharmacological studies have shown that flaxseed has a variety of biological activities, including anti-tumor effects, antioxidant effects, immunosuppressive effects, and hypoglycemic and blood lipid-lowering effects. Flaxseed is flat oval, 4-7mm long, 2.5mm wide, 1.5mm thick, and 1,000-grain weight is 3.5-11g. Flaxseed consists of a skin, husk and cotyledons. The thickness of the epidermis is 0.1-0.2mm, and it is mainly composed of carbohydrate complex compounds such as starch. The cotyledons account for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/30
CPCC12P21/06C07K1/30
Inventor 胡永强周同永赵永解
Owner QINGHAI SCI TECH INFORMATION RES INST