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A preparation method of chlorella antioxidative polypeptide

An anti-oxidant peptide and chlorella technology, applied in the biological field, to improve the extraction rate, improve the ability to scavenge DPPH and hydroxyl free radicals in vitro, and scavenge the active oxygen species of liver cancer cells

Active Publication Date: 2021-12-17
福建宸润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alzahrani et al. extracted three kinds of microalgae proteins by ultrasonic method in 2017, and then separately enzymolyzed them with alkaline protease, flavor protease and trypsin, and compared the antioxidant effects of these enzymatic hydrolyzed products. antioxidant activity, but only the peptide hydrolyzed by alkaline protease has the best antioxidant activity

Method used

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  • A preparation method of chlorella antioxidative polypeptide
  • A preparation method of chlorella antioxidative polypeptide
  • A preparation method of chlorella antioxidative polypeptide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of Chlorella cell wall enzymatic hydrolyzate and specific protein enzymatic hydrolyzate

[0031] The cellulase, pectinase and various proteases used were purchased from Beijing Suolaibao Technology Co., Ltd.

[0032] 1) The composition ratio of the chlorella cell wall enzymatic hydrolyzate is:

[0033]

[0034] After the preparation of the chlorella cell wall enzymatic hydrolyzate, the pH was adjusted to 4.0 with concentrated hydrochloric acid with a mass fraction of 36%.

[0035] 2) The proportioning of the specific proteolytic solution components is:

[0036]

[0037]

[0038] After the preparation of the above-mentioned specific proteolysis solution, adjust the pH to 9.5 with 1mol / L NaOH solution;

[0039] 2. Chlorella antioxidant polypeptide is prepared by the following steps:

[0040] 1) Weigh 500g of chlorella powder and add it to 4.5L of 0.3mol / L NaOH dilute alkali solution, and soak for 60min at 60°C to obtain the pretreated chlorella ...

Embodiment 2

[0064] 1. Preparation of Chlorella cell wall enzymatic hydrolyzate and specific protein enzymatic hydrolyzate

[0065] The cellulase, pectinase and various proteases used were purchased from Beijing Suolaibao Technology Co., Ltd.

[0066] 1) The weight ratio of the chlorella cell wall enzymatic hydrolyzate is:

[0067]

[0068]

[0069] Adjust the pH to 4.0 with concentrated hydrochloric acid with a mass fraction of 36%;

[0070] 2) The weight ratio of the specific proteolytic solution is:

[0071]

[0072] Adjust the pH to 7.5-9.5 with 1mol / L NaOH solution;

[0073] 2. Prepared by the following steps:

[0074] 1) Weigh 750g of chlorella powder and add it to 4.25L of 0.3mol / L NaOH dilute alkali solution, and soak for 60min at 60°C to obtain the pretreated chlorella liquid;

[0075]2) The pretreated chlorella liquid obtained in step 1) was homogenized under high pressure twice under a pressure of 55MPa, centrifuged at 5000rpm for 20min, and the obtained supernatant...

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Abstract

The invention provides a preparation method of chlorella anti-oxidation polypeptide, which is characterized in that the chlorella protein is extracted by high-pressure homogenization combined with cellulase / pectinase enzymolysis, and after enzymolysis by a specific proteolysis solution, Separation by ultrafiltration membrane to obtain chlorella enzymatic peptides in different molecular weight ranges. The chlorella polypeptide with a molecular weight range of 0-3.5 KDa after enzymolysis by a specific proteolysis solution of the present invention has better ability to scavenge DPPH and hydroxyl free radicals in vitro than other protease hydrolysis products and chlorella polypeptides in other molecular weight ranges , the ability to scavenge reactive oxygen species in liver cancer cells in vitro, the more effective ability to inhibit the formation of malondialdehyde, the peroxidation product in animals, and the ability to promote glutathione peroxidase activity. The invention relies on the specific protease hydrolyzate to prepare the chlorella active peptide with better anti-oxidation function, which is beneficial to the development and utilization of anti-oxidation function health food and medical products.

Description

technical field [0001] The invention provides a preparation method of chlorella anti-oxidation polypeptide, which belongs to the field of biotechnology. Background technique [0002] Chlorella (Chlorella) is a universal single-celled green algae, which is widely distributed in nature. It can grow and reproduce by using organic carbon sources under heterotrophic conditions. Creatures that grow 4x per hour. Chlorella is extremely nutritious, in which the crude protein content is as high as 50%, the composition of essential amino acids is basically balanced, and it also contains unsaturated fatty acids, biologically active polysaccharides, nucleic acids, vitamins, trace elements, minerals, chlorophyll, etc., with extremely high nutritional value , has various biopharmacological activities such as preventing and treating peptic ulcer, anti-tumor, enhancing immunity, anti-radiation, anti-pathogenic microorganisms, preventing and treating anemia, lowering blood lipid and anti-ath...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34Y02P20/10
Inventor 林陈胜张彦定
Owner 福建宸润生物科技有限公司