Purpose of fructus citrus sarcodactylis volatile oil

A technology of volatile oil and bergamot, which is applied in the field of prevention and treatment of bergamot volatile oil on Chinese medicinal materials or crop root rot, can solve the problems of no bergamot volatile oil, etc., and achieve the effects of low residue, ecological environment protection, and low reagent cost

Active Publication Date: 2019-01-22
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the use of bergamot essential oil in the control of crop diseases and insect pests

Method used

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  • Purpose of fructus citrus sarcodactylis volatile oil
  • Purpose of fructus citrus sarcodactylis volatile oil
  • Purpose of fructus citrus sarcodactylis volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of bergamot volatile oil

[0020] (1) Add 8 times the powder mass of water to 300 g of pulverized bergamot powder, soak for 2 hours, use steam to extract the volatile oil in male cloves by means of heating and distillation, and the extraction time is 6 hours, and condense to collect the effluent;

[0021] (2) The extracted volatile oil was dried with anhydrous sodium sulfate to remove the contained water, put into a brown sample bottle, sealed, and stored at -20°C until use.

Embodiment 2

[0022] Embodiment 2: the activation of pathogenic bacteria

[0023] (1) Isolate the root rot pathogenic bacteria from the roots of Panax notoginseng, purify and identify it as Fusarium oxysporum ( Fusarium oxysporum ), Fusarium solani ( Fusarium solani ) and Cylindrosporium destroyer ( Cylindrocarpon destructans ), inoculate the strains on the PDA medium for activation, after inoculating 4 times, take the vigorously growing strains for use;

[0024] (2) Potato dextrose agar medium (PDA): 200 g potato, 20 g glucose, 20 g agar, 1000 mL distilled water.

Embodiment 3

[0025] Embodiment 3: GC-MS measures the chemical composition of bergamot volatile oil

[0026] (1) GC conditions: Instrument model: Agilengt Technologies 7890B-5977B; Chromatographic column: HP-5MS 30 m×250μm×0.25μm;

[0027] (2) EI source Ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30~500m / z; inlet temperature: 285°C; split ratio 10:1; injection volume: 1 μl; Electron energy: 70eV; column oven temperature rise program: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the continuous carbon (C9-C25) of alkanes is calculated, and the retention time and mass spectrum of the compound are compared with the NIST17.L database to determine the final compound. The test results are shown in the table below;

[0028]

[0029] a: retention time; b: relative peak area±relative deviation; c: retention index.

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Abstract

The invention discloses a novel purpose of fructus citrus sarcodactylis volatile oil, namely application of the fructus citrus sarcodactylis volatile oil to the preparation of a chemical fertilizer ora pesticide for preventing and treating the root rot of a traditional Chinese medicinal material or a crop. Shown by an experimental result, the fructus citrus sarcodactylis volatile oil can be usedfor inhibiting the growth of mycelia of three main pathogenic bacteria, which cause the occurrence of the root rot of radix notoginseng, of fusarium oxysporum, fusarium solani and cylindrocarpon destructans; further, the fructus citrus sarcodactylis volatile oil is compounded with hymexazol to have additive and synergistic effects on a bacterial strain; the fructus citrus sarcodactylis volatile oil is added into continuous cropping soil, so that the morbidity and the disease index of the root rot of the radix notoginseng can be decreased; and as the volatile oil has effumability and further has fragrant and dirt-avoiding characteristics, the chemical fertilizer or the pesticide developed with the fructus citrus sarcodactylis volatile oil has the advantages of safety, effectiveness and lowresidue and the like.

Description

technical field [0001] The invention relates to a new application of bergamot volatile oil, in particular to the prevention and control effect of bergamot volatile oil on Chinese medicinal materials or crop root rot, and belongs to the technical field of biopesticides. Background technique [0002] Panax notoginseng ( Panax notoginseng (Burk.) F.H.Chen), also known as Tianqi, is a perennial herb of Araliaceae ginseng. It has the functions of dispelling blood stasis and hemostasis, reducing swelling and relieving pain. It is a rare and precious cultivated medicinal plant unique to my country. , which has a cultivation history of more than 400 years in Wenshan Prefecture, Yunnan Province. The roots, stems, leaves, and flowers of Panax notoginseng can be used as medicine. The range of medicines is very wide, and the social demand is constantly increasing. With the rapid increase of the planting area of ​​Panax notoginseng, its disease problem is becoming more and more serious,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/36A01P3/00C09K17/14C09K101/00
CPCA01N65/00A01N65/36C09K17/14C09K2101/00
Inventor 董鲜程永现徐福荣陈传娇
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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