Preparation method of wheat bran lactic acid fermented slice rich in viable organisms
A technology of lactic acid fermentation and bran, applied in the field of preparation of bran lactic acid fermentation tablets, can solve the problems of low product utilization rate, indigestion and absorption, rough taste, etc., to maintain the balance of flora, promote absorption, reduce cholesterol and blood sugar effect
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[0035] 1. Activation of lactic acid bacteria
[0036] Add 10 mL of MRS solid medium to two test tubes, sterilize at 121 °C for 20 min, place on an inclined plane to cool to 40 °C, and put 0.1 mL of glycerol-preserved LP strain bacterial liquid into one test tube on a sterile operating table. The other branch was inserted with 0.1 mL glycerol-preserved LF strain bacterial liquid, and cultured at 37°C for 24 hours. Then the MRS liquid medium was sterilized at 121°C for 20 minutes, and after cooling, the colonies of LP strain and LF strain that grew well on the slant medium were picked in an aseptic operating table, inoculated into the MRS liquid medium, and incubated at 37°C for 24 hours.
[0037] 2. High density culture of lactic acid bacteria
[0038] Inoculate the LP strain activated in step 1 with an inoculum amount of 1% in the sterilized LP growth medium and culture it at 37°C for 24 hours, collect the bacterial precipitate after centrifuging at 4000rpm for 10min, suspend...
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