A step-by-step method for preparing microfibrillar collagen using gradient polarity of ionic liquids

Abstract

The invention provides a method for preparing micro-fiber collagen by utilizing ion liquid in different steps. The method comprises the following steps: separately preparing at least three ion liquidwith the mass concentration of 5 to 95 percent, sequencing the ion liquid according to the polarity of the ion liquid from small to big or from big to small, successively adding a raw material of micro-fiber collagen into the ion liquid in different steps, performing the ion liquid treatment, and performing the after-treatment, thus obtaining the micro-fiber collagen. The preparation method is mild in conditions, and by regulating and controlling the polarity of the ion liquid at the temperature less than or equal to a denaturation temperature of the collagen, the micro-fiber collagen with thebiological activity can be obtained under a non-denaturation of the collagen; and the yield of the preparation method can reach 80 percent, the ion liquid can be recycled and reused, the prepared micro-fiber collagen has the characteristics of high short fiber content, high purity, good biological compatibility and nontoxicity, and can be directly used as a biological medical material in the field of biological medicine.

Description

technical field [0001] The invention belongs to the technical field of biological material preparation, and relates to a method for preparing microfibril collagen step by step using gradient polarity of ionic liquid. Background technique [0002] Collagen is a kind of water-insoluble fibrin, which is the skeleton of the extracellular matrix. It exists widely in the connective tissue of vertebrates (including soft tissue, skin, tendon, bone and cartilage), and plays a pivotal role in the regulation of the normal physiological functions of organisms. role. At the same time, as a natural polymer material, collagen has biocompatibility, biodegradability and bioactivity unmatched by other synthetic materials, can promote cell growth and platelet aggregation, and has relatively low immunogenicity. The denaturation temperature of collagen in solution state is 38-40°C, and the denaturation temperature of mammalian skin collagen varies slightly with the source of the material, gener...

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Problems solved by technology

[0006] 1. The temperature in the process of preparing microfibrous collagen is 90-130°C, far exceeding the denaturation temperature of collagen, which loses its biological activity and cannot be used in the field of biomedicine;

[0007] 2. It adopts ionic liquid / dimethyl sulfoxide system to prepare microfibril collagen. Dimethyl sulfoxide has certain toxicity and is difficult to completely remove, so the prepared microfibril collagen cannot be applied to biomedical materials;

[0008] 3. The prepared microfibrous collagen is not sterilized and has certain biological toxicity;

[0009] 4. The content of short fibers (fiber length ≤ 1 mm) in the prepared microfibrous collagen is insufficient, does not meet the standards of biomedical materials, and the yield is not high

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