Preparation method and applications of animal-derived bleeding stopping dressing

The technology of hemostatic dressing and hemostatic material, which is applied in the field of medical biomedical materials, can solve the problems of wound tissue damage, limited hemostasis of hemostatic material, less promotion of healing, etc., and achieves portability, reduction of labor cost and time cost, and biocompatibility. good effect

Inactive Publication Date: 2019-02-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The QuikClot zeolite-based hemostatic powder (Z-Medica Company) approved by the US FDA is used for first aid in severe bleeding trauma, but the high temperature exceeding 100°C causes damage to the wound tissue when used
Most of the existing hemostatic materials are limited to simple hemostasis, and there are relatively few studies on promoting healing

Method used

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  • Preparation method and applications of animal-derived bleeding stopping dressing
  • Preparation method and applications of animal-derived bleeding stopping dressing
  • Preparation method and applications of animal-derived bleeding stopping dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of burnt cocoon powder hemostatic dressing

[0035] 1) silkworm cocoon is removed silkworm chrysalis, is raw material with the silkworm cocoon of removing silkworm chrysalis;

[0036] 2) Bake the hemostatic raw materials at 200°C for 2 hours or 230°C for 30 minutes (or 230°C for 2 hours), subject to no carbonization or preservation of hemostatic activity (such as earthy yellow or brownish yellow, with a water content of about 4 %);

[0037] 3) the roasted de-chrysalis burnt silkworm cocoons are directly powdered;

[0038] 4) The powdered burnt cocoons are sterilized by Co-60 irradiation for 10-30 hours or treated by other sterilization methods.

Embodiment 2

[0039] Example 2 The following is the appearance of the burnt silkworm cocoon hemostatic dressing prepared by the present invention

[0040] figure 1 Among them, a is the sample appearance of the complete burnt silkworm cocoon without pupae, b is the sample appearance of the powdered burnt silkworm cocoon hemostatic dressing, and c is the scanning electron microscope image of the powdery burnt silkworm cocoon hemostatic dressing sample.

Embodiment 3

[0041] Example 3 Cytotoxicity experiment of burnt cocoon powder hemostatic dressing

[0042] Preparation method of extract solution: All operations in this experiment were completed in a biological safety cabinet. The ratio of the extracting liquid shall be carried out according to the requirements in the sample preparation and reference samples in Part 12 of the National Standard for Biological Evaluation of Medical Devices. The extraction shall be carried out in a sterile, chemically inert closed container using aseptic techniques. Immerse the sterilized burnt silkworm cocoon powder in the serum-free RPMI 1640 culture solution at a ratio of 0.1g / mL, seal it with a parafilm and place it at a constant temperature of 37°C for 24h extraction. After taking out the sample, use 0.2μm sterile filter to remove particulate matter from the leachate.

[0043] Cell culture and detection: Digest the passaged L929 cells with trypsin, add fresh culture medium, pipette repeatedly to make th...

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PUM

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Abstract

The invention belongs to the field of medical biomedical materials, and particularly relates to a preparation method and applications of an animal-derived bleeding stopping dressing, wherein the animal-derived bleeding stopping dressing has advantages of good safety, good biocompatibility, no irritation to skin and rapid aggregation of platelets and red blood cells. According to the preparation method, Silkworm Chrysalis-removed silkworm cocoon or silkworm cocoon sheet is used as a raw material; the whole silkworm cocoon or silkworm cocoon sheet is placed in an oven, and is baked to achieve the optimal bleeding stopping effect, wherein the silkworm cocoon or silkworm cocoon sheet is not blackened and charred or the bleeding stopping activity is retained after the baking; and after the baking, the whole silkworm cocoon or silkworm cocoon sheet is grinding into powder, and sterilization packaging is performed to obtain the bleeding stopping dressing with strong bleeding stopping effect.According to the present invention, the coke silkworm cocoon bleeding stopping material has advantages of high biocompatibility and significant bleeding stopping effect, can be used as the bleeding stopping material for local bleeding on the surface of the skin, and further has effects of rapid water absorption and bleeding stopping, rapid red blood cell agglutination and bleeding stopping, and high safety.

Description

technical field [0001] The invention belongs to the field of medical biomedical materials, and in particular relates to a method for preparing an animal-derived hemostatic dressing and its application. The dressing has good safety, biocompatibility, no irritation to the skin, and the performance of rapidly aggregating platelets and red blood cells. Background technique [0002] The development and improvement of hemostatic materials has become a research hotspot in the field of medicine. With the continuous advancement of medical technology, new medical materials and related technologies have been well developed. [0003] At present, the commonly used effective hemostasis method is to use absorbable materials with good biocompatibility to stop bleeding. The main characteristics of ideal hemostatic materials are generally: good safety, insensitivity, and non-allergies; Good hygroscopic properties; no need to be replaced frequently; high cost performance; not easy to adhere t...

Claims

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Application Information

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IPC IPC(8): A61L24/00
CPCA61L24/0005A61L24/0015A61L2300/30A61L2300/418A61L2400/04
Inventor 杨英陈智朱海红
Owner ZHEJIANG UNIV
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