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Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology

A nano-starch and bio-enzyme technology, which is applied in the field of preparation of nano-starch granules, can solve the problems of difficult operation, high environmental requirements, and large product particle size changes, and achieve the effects of reducing production costs, low environmental requirements, and uniform granules

Inactive Publication Date: 2019-02-01
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Someone added the starch solution dropwise to n-hexane containing Span-60 to form a microemulsion, and used epichlorohydrin as a cross-linking agent to obtain starch microspheres with an average particle size of 92.2nm, but the cross-linking agent was not easy to remove.
[0006] "Study on Enzymatic Preparation Process Optimization of Rice Microporous Starch" ("Grain and Oil Processing", No. 5, 2007, P104-106), discloses a method of directly hydrolyzing starch solution with amylase to obtain non-nano-scale microporous starch Although the specific surface area is increased to a certain extent, it is much smaller than that of nano-starch, which directly leads to a great reduction in the adsorption, embedding and drug-loading capacity of starch granules.
[0007] CN104804200 discloses a method for preparing nano-starch by biological enzyme method. The method has high requirements on the environment and strict temperature restrictions. Grade starch granules, difficult to operate
Moreover, although the product obtained by this technology is at the nanometer level, the particles are still relatively large

Method used

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  • Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology
  • Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology
  • Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Dissolve 5g of rice starch in 100mL of distilled water to make a 5% starch suspension;

[0035] (2) Heat and stir the starch suspension in step (1) in a boiling water bath at 70°C for 15 minutes to gelatinize to form a starch solution;

[0036] (3) Dissolve 2.21 g of disodium hydrogen phosphate and 0.75 g of citric acid in 100 mL of distilled water, mix thoroughly to prepare a buffer, use the prepared buffer to adjust the starch solution in step (2) to a pH of 4.5, and add 6u / g of α-amylase, hydrolyzed in a constant temperature water bath at 50°C for 5 hours, centrifuged at 4000r / min for 15 minutes, washed and precipitated with ethanol for 3 times, and freeze-dried in a vacuum freeze dryer to obtain short amylose;

[0037] (4) Add short amylose in step (3) to 100mL urea / sodium hydroxide / distilled water with a mass ratio of 6:2:100, and mechanically stir for 3 hours at 500r / min;

[0038] (5) Freezing: freeze at -20°C for 24 hours;

[0039] (6) Thawing: thaw at roo...

Embodiment 2

[0046] (1) Dissolve 2g of rice starch in 100mL of distilled water to make a 2% starch suspension;

[0047] (2) Heat and stir the starch suspension in step (1) in a boiling water bath at 100°C for 20 minutes to gelatinize to form a starch solution;

[0048] (3) Dissolve 1.8g of citric acid and 0.41g of sodium citrate in 100mL distilled water to form a buffer, use the buffer to adjust the starch solution in step (2) to pH 5, add 12u / g pullulanase, Hydrolyze in a water bath at 50°C for 8 hours, centrifuge at 4000r / min for 15 minutes, wash the precipitate with ethanol 4 times, and vacuum freeze-dry to obtain short amylose;

[0049] (4) Add short amylose in step (3) to 100mL urea / sodium hydroxide / distilled water with a mass ratio of 6:4:100, and mechanically stir for 3 hours at 800r / min;

[0050] (5) Freezing: freeze at -80°C for 48 hours;

[0051] (6) Thawing: thaw at room temperature until completely thawed, and continue mechanical stirring at 400r / min for 40min;

[0052] (7) ...

Embodiment 3

[0055] (1) Dissolve 10g of rice starch in 100mL of distilled water to make a 10% starch suspension;

[0056] (2) Heat and stir the starch suspension in step (1) in a boiling water bath at 100°C for 10 minutes to gelatinize to form a starch solution;

[0057] (3) Dissolve 2.1g of citric acid, 0.83g of sodium hydroxide, and 1.06mL of commercially available concentrated hydrochloric acid in 100mL of distilled water to form a buffer. Use this buffer to adjust the starch solution in step (2) to a pH of 5.5, and add 8u / g of β-amylase, hydrolyzed in a water bath at 50°C for 5h, centrifuged at 4000r / min for 15min, washed and precipitated with ethanol 3 times, and vacuum freeze-dried to obtain short amylose;

[0058] (4) Add short amylose in step (3) to 100mL of urea / sodium hydroxide / distilled water solution with a mass ratio of 4:4:100, and mechanically stir for 2 hours at 500r / min;

[0059] (5) Freezing: freeze at -60°C for 36 hours;

[0060] (6) Thawing: thaw at room temperature ...

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Abstract

The invention relates to a method for preparing nano starch granules by combining biological enzyme and an alkali freezing technology. The method comprises the following steps of: dissolving starch inwater to prepare a starch suspension; heating and stirring the starch suspension in a boiling water bath until the materials are completely gelatinized to form a starch solution; adjusting the starchsolution with a buffer solution to a pH value being 4.5-5.5, adding bio-enzyme and performing hydrolyzing in a thermostatic water bath, centrifuging the material, washing the precipitate with ethanol, performing vacuum freeze drying, and obtaining short amylase; adding the short amylose to a urea / sodium hydroxide solution, and mechanically stirring the materials in an ice salt bath; freezing thematerial; unfreezing the material at room temperature to complete melting the material, and performing continuous mechanical stirring; performing dialysis, and performing vacuum freeze drying on a dialysate obtained by dialysis to obtain nano starch granules. The equipment required for the method is simple, and the product particle size is easy to control.

Description

technical field [0001] The invention relates to a method for preparing nano starch granules, in particular to a method for preparing nano starch granules by combining biological enzymes with alkali freezing technology. Background technique [0002] In recent years, nanoparticles have become the focus of scientific research because of their large specific surface area, strong adsorption capacity, quantum effect and size effect. The application of nanoparticles involves many industries, and the nanoparticles used in the fields of food, medicine and biotechnology must be non-toxic, and have good biocompatibility and biodegradability. Therefore, natural polysaccharide polymers such as starch, cellulose, and chitosan have become ideal materials for preparing nanoparticles. Among them, starch has a wide range of sources, low price, no biological toxicity and no immunogenicity, and has broad market prospects, especially It has been widely used in the field of biomedicine. [0003...

Claims

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Application Information

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IPC IPC(8): C08J3/16C08L3/12
CPCC08J3/16C08J2303/12
Inventor 肖华西杨帆张倩林亲录刘高强张琳许东唐玮泽丁玉琴王素燕
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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