Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology

A nano-starch and bio-enzyme technology, which is applied in the field of preparation of nano-starch granules, can solve the problems of difficult operation, high environmental requirements, and large product particle size changes, and achieve the effects of reducing production costs, low environmental requirements, and uniform granules

Inactive Publication Date: 2019-02-01
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Someone added the starch solution dropwise to n-hexane containing Span-60 to form a microemulsion, and used epichlorohydrin as a cross-linking agent to obtain starch microspheres with an average particle size of 92.2nm, but the cross-linking agent was not easy to remove.
[0006] "Study on Enzymatic Preparation Process Optimization of Rice Microporous Starch" ("Grain and Oil Processing", No. 5, 2007, P104-106), discloses a method of directly hydrolyzing starch solution with amylase to obtain non-nano-scale microporous starch Although the specific surf

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology
  • Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology
  • Method for preparing nano starch granules by combining biological enzyme and alkali freezing technology

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1:

[0034] (1) Dissolve 5g of rice starch in 100mL of distilled water to prepare a 5% starch suspension;

[0035] (2) The starch suspension in step (1) is heated and stirred in a boiling water bath at 70°C for 15 minutes to gelatinize to form a starch solution;

[0036] (3) Dissolve 2.21g of disodium hydrogen phosphate and 0.75g of citric acid in 100mL of distilled water, mix thoroughly to prepare a buffer solution, adjust the starch solution in step (2) with the prepared buffer solution to pH 4.5, add 6u / g of ɑ-amylase was hydrolyzed in a constant temperature water bath at 50℃ for 5h, centrifuged at 4000r / min for 15min, washed with ethanol for 3 times, put into a vacuum freeze dryer and freeze-dried to obtain short amylose;

[0037] (4) Add the short amylose in step (3) to a mixed solution with a mass ratio of 100 mL of urea / sodium hydroxide / distilled water of 6:2:100, and mechanically stir for 3h under the condition of 500r / min;

[0038] (5) Freezing: freezing at -20...

Example Embodiment

[0045] Example 2:

[0046] (1) Dissolve 2g of rice starch in 100mL of distilled water to form a 2% starch suspension;

[0047] (2) The starch suspension in step (1) is heated and stirred in a boiling water bath at 100°C for 20 minutes to gelatinize to form a starch solution;

[0048] (3) Dissolve 1.8 g of citric acid and 0.41 g of sodium citrate in 100 mL of distilled water to form a buffer solution, use the buffer solution to adjust the starch solution in step (2) to pH 5, add 12 u / g pullulanase, Hydrolyze in a 50℃ water bath for 8 hours, centrifuge at 4000r / min for 15 minutes, wash the precipitate 4 times with ethanol, and freeze-dry in vacuum to obtain short amylose;

[0049] (4) Add the medium and short amylose in step (3) to a mixed solution with a mass ratio of 100 mL of urea / sodium hydroxide / distilled water of 6:4:100, and mechanically stir for 3 hours at 800r / min;

[0050] (5) Freezing: freezing at -80°C for 48 hours;

[0051] (6) Thaw: Thaw at room temperature to complete thawi...

Example Embodiment

[0054] Example 3:

[0055] (1) Dissolve 10g of rice starch in 100mL of distilled water to prepare a 10% starch suspension;

[0056] (2) The starch suspension in step (1) is heated and stirred in a boiling water bath at 100°C for 10 minutes to gelatinize to form a starch solution;

[0057] (3) Dissolve 2.1 g of citric acid, 0.83 g of sodium hydroxide, and 1.06 mL of commercially available concentrated hydrochloric acid in 100 mL of distilled water to form a buffer solution. Use the buffer solution to adjust the starch solution in step (2) to a pH of 5.5 and add 8 u / g of β-amylase, hydrolyzed in a water bath at 50℃ for 5h, centrifuged at 4000r / min for 15min, washed with ethanol 3 times, and freeze-dried in vacuum to obtain short amylose;

[0058] (4) Add the medium and short amylose in step (3) to a solution with a mass ratio of 100 mL of urea / sodium hydroxide / distilled water of 4:4:100, and mechanically stir for 2h under the condition of 500r / min;

[0059] (5) Freezing: freezing at -60...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for preparing nano starch granules by combining biological enzyme and an alkali freezing technology. The method comprises the following steps of: dissolving starch inwater to prepare a starch suspension; heating and stirring the starch suspension in a boiling water bath until the materials are completely gelatinized to form a starch solution; adjusting the starchsolution with a buffer solution to a pH value being 4.5-5.5, adding bio-enzyme and performing hydrolyzing in a thermostatic water bath, centrifuging the material, washing the precipitate with ethanol, performing vacuum freeze drying, and obtaining short amylase; adding the short amylose to a urea/sodium hydroxide solution, and mechanically stirring the materials in an ice salt bath; freezing thematerial; unfreezing the material at room temperature to complete melting the material, and performing continuous mechanical stirring; performing dialysis, and performing vacuum freeze drying on a dialysate obtained by dialysis to obtain nano starch granules. The equipment required for the method is simple, and the product particle size is easy to control.

Description

technical field [0001] The invention relates to a method for preparing nano starch granules, in particular to a method for preparing nano starch granules by combining biological enzymes with alkali freezing technology. Background technique [0002] In recent years, nanoparticles have become the focus of scientific research because of their large specific surface area, strong adsorption capacity, quantum effect and size effect. The application of nanoparticles involves many industries, and the nanoparticles used in the fields of food, medicine and biotechnology must be non-toxic, and have good biocompatibility and biodegradability. Therefore, natural polysaccharide polymers such as starch, cellulose, and chitosan have become ideal materials for preparing nanoparticles. Among them, starch has a wide range of sources, low price, no biological toxicity and no immunogenicity, and has broad market prospects, especially It has been widely used in the field of biomedicine. [0003...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08J3/16C08L3/12
CPCC08J3/16C08J2303/12
Inventor 肖华西杨帆张倩林亲录刘高强张琳许东唐玮泽丁玉琴王素燕
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap