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Fluorescent staining solution of detecting fungi

A fluorescent dyeing and fungus technology, applied in the field of detection, can solve the problems of high professional skills requirements, many reagents required for matching, troublesome storage management, etc., and achieve the effects of wide application range, convenient identification of fungi, and high detection rate

Inactive Publication Date: 2019-02-01
江西业力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages are: 1. The requirements for the professional skills of the operators are relatively high
2. The operation is cumbersome and takes a long time (about 1 hour) 3. Many reagents are required for matching, and storage management is troublesome

Method used

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  • Fluorescent staining solution of detecting fungi
  • Fluorescent staining solution of detecting fungi
  • Fluorescent staining solution of detecting fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Fluorescent staining solution is made by mixing the following formula according to weight percentage: fluorescent whitening agent 280.001%, potassium hydroxide 10%, dimethyl sulfoxide 4%, sodium chloride 3.6%, glycerin 10%, Evans blue 0.01%, The balance is purified water; the method for configuring the fluorescent staining solution is:

[0039] (1) Take the corresponding weight of potassium hydroxide and add part of water to fully stir and dissolve to dissipate heat. After cooling to room temperature, add dimethyl sulfoxide, sodium chloride and glycerin, and stir until there is no stratification. Exothermic, cooled to room temperature for subsequent use, to obtain a transparent colorless clear liquid, named as liquid A;

[0040] (2) Weigh the fluorescent whitening agent 28 of the corresponding weight and add it into some water to fully stir and dissolve it. It should be a transparent colorless or light yellow clear liquid, named as liquid B;

[0041] (3) take by weighi...

Embodiment 2

[0046] The fluorescent staining solution without adding sodium chloride is made by mixing the following formula according to weight percentage: 280.001% fluorescent whitening agent, 10% potassium hydroxide, 4% dimethyl sulfoxide, 10% glycerin, 0.01% Evans blue, The balance is purified water; the method for configuring the fluorescent staining solution is:

[0047] (1) Weigh the corresponding weight of potassium hydroxide and add some water to fully stir to dissolve and dissipate heat. After cooling to room temperature, add dimethyl sulfoxide and glycerin and stir until there is no stratification. At this time, heat will be released and cooled. To room temperature for subsequent use, a transparent, colorless and clear liquid is obtained, which is named as liquid A;

[0048] (2) Weigh the fluorescent whitening agent 28 of the corresponding weight and add it into some water to fully stir and dissolve it. It should be a transparent colorless or light yellow clear liquid, named as ...

Embodiment 3

[0054] 197 samples of vaginal secretions with KOH wet film microscopic examination results were obtained from the hospital, and the "gold standard" fungal culture method, fungal fluorescent staining microscopic examination and fluorescent staining solution without adding sodium chloride microscopic examination were carried out at the same time. The operation steps are:

[0055] 1. Fungal culture method

[0056] 1. Turn on the ultraviolet light of the ultra-clean workbench for disinfection and sterilization half an hour in advance, and clean and disinfect the operator's clothes and hands.

[0057] 2. Take out the prepared culture medium and number it correspondingly to the gynecological sample.

[0058] 3. According to the corresponding number, open the plate and drop a drop of sterilized physiological saline on it, dip the sampling swab in physiological saline and spread it evenly on the plate, cover the petri dish, and put the sampling swab back to its original place. tube....

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Abstract

The invention belongs to the field of detection, and particularly relates to a configuring method of a fluorescent staining solution of detecting fungi. The fluorescent staining solution is formed by0.001-0.003% of a fluorescent whitening agent 28, 5-15% of potassium hydroxide, 3-6%of dimethyl sulfoxide, 2-5% of sodium chloride, 5-15% of glycerol, 0.01-0.03% of Evans blue, and the balance of purified water. Hypha and spore structures of the fluorescent staining solution provided by the invention are clearly visible, fungus identification is easy, sensitivity is high, accuracy and efficiency of staining result interpretation are improved, a detection rate is high, and an application range is wide.

Description

technical field [0001] The invention belongs to the field of detection, and in particular relates to a method for configuring a fluorescent staining solution for detecting fungi. Background technique [0002] Fungi belong to eukaryotes, without plastids, and their nutritional mode is absorption without phagocytosis. The cell wall contains chitin and β-glucan. It can cause human and animal diseases, called fungal diseases, and can also cause plant diseases, human allergic diseases and mycotoxin poisoning. Fungal invasion of the human body is often secondary to other diseases, such as chronic wasting diseases such as malignant tumors and diabetes; large doses of X-ray irradiation; immunosuppressants, etc. lead to low immune function and resistance; The reaction is mostly suppressed, and it is also easy to be infected with fungi; in addition, organ transplantation, catheter intubation, radiotherapy and other applications cause local tissue damage, which provides conditions fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 熊文芳吕正和陈永洪杜厚明谢赦飞柯子旋
Owner 江西业力医疗器械有限公司
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