Paenibacillus elgii strain for producing polysaccharide with effect of after-sun repair and application of paenibacillus elgii strain

A Bacillus sp., post-sun repair technology, applied in bacteria, cosmetic preparations, preparations for skin care, etc. Active ingredients are easily available and inexpensive

Active Publication Date: 2019-02-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, UV may induce cells to produce excessive reactive oxygen species (ROS), destroy their own antioxidant defense system, cause lipid peroxi

Method used

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  • Paenibacillus elgii strain for producing polysaccharide with effect of after-sun repair and application of paenibacillus elgii strain
  • Paenibacillus elgii strain for producing polysaccharide with effect of after-sun repair and application of paenibacillus elgii strain
  • Paenibacillus elgii strain for producing polysaccharide with effect of after-sun repair and application of paenibacillus elgii strain

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Example 1: Extraction and content determination of exopolysaccharides produced by the above strains

[0023] An appropriate amount of the above strains is picked from the sugar-producing solid medium and inoculated into the sugar-producing fermentation medium. At 30°C, shake culture at 150 rpm for 48 hours to obtain a fermentation broth. figure 1 It is the plate culture trait of the bacterial strain PYQ1 of the present invention.

[0024] Among them, the used sugar-producing solid medium components are: sucrose 20g / L, tryptone 10g / L, yeast powder 5g / L, Na 2 HPO 4 12H 2 O 3g / L, agar 20g / L, and the balance is distilled water. The components of the sugar-producing fermentation broth used are: sucrose 50g / L, tryptone 5g / L, yeast powder 1g / L, Na 2 HPO 4 12H 2 O 3g / L, the balance is distilled water.

[0025] The above fermentation broth was centrifuged at 4000 g for 10 min to remove the bacteria to obtain the supernatant. Add 1 / 4 volume of Sevage solution (n-butanol:...

example 2

[0027] Example 2: Identification of microbial strains of the present invention

[0028] Such as figure 2 The strain shown is purple by Gram stain and is Gram-positive. According to the steps of TakaraBacteria Genomic DNA Extraction Kit (TakaraBacteria Genomic DNA Extraction Kit) to extract the total DNA of the strains described in the present invention, amplify the 16S rDNA gene of the bacteria with the general primers 27F and 1492R, recover the amplified products and carry out Sequencing, identification of bacterial species by determining the sequence of 16S rDNA. The obtained sequence results were compared by Blast at NCBI, and the recognized standard sequence data homologous to the 16S rDNA of the strain was obtained from the GenBank database. The sequence similarity was calculated using MEGA software and the Neighbor-Joining algorithm (Neighbor-Joining) was used as the system developmental analysis.

[0029] The phylogenetic tree of the constructed strain is as follows...

example 3

[0030] Example 3: Use of the above-mentioned polysaccharides as a repairing agent for sun damage

[0031] HaCaT cells in the logarithmic phase were collected and inoculated in a 96-well plate with 5000 cells per well, and cultured in a 37°C, 5% CO2 incubator. Cells were divided into control group, UV model group, polysaccharide group (1mg / mL), octocrylene group (8%) and Vc group (1mg / mL), wherein the latter two groups were positive controls, and each group repeated 3 wells. After the cells were cultured for 24 hours, the culture medium was replaced with phosphate buffered saline (PBS) and irradiated with UV light, and the control group was wrapped with tinfoil to avoid light. Aspirate the PBS and add the culture medium, wherein the culture medium of the polysaccharide group contains 1 mg / mL of the prolific exopolysaccharide of the above strains, and the culture medium of the octocrylene group contains 8% 2-cyano-3,3-di Isooctyl phenylacrylate, Vc group medium contains 1mg / mL ...

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Abstract

The invention discloses a paenibacillus elgii strain for producing polysaccharide with an effect of after-sun repair and application of the paenibacillus elgii strain. A class name of the strain is paenibacillus polymyxa PYQ1, and the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No. 16444. A preparation method of the paenibacillus elgii strain comprises the following steps of isolated culture of a strain and 16S rDNA identification; and acquisition of extracellular polysaccharide serving as a fermentation product ofthe strain and application of the extracellular polysaccharide produced by the strain in after-sun damage repair. The polysaccharide produced by the paenibacillus elgii PYQ1 disclosed by the inventioncan remarkably improve the proliferative activity of after-sun damaged cells, has obvious effect of after-sun damage repair, has the characteristics of low cost, simpleness and easiness in acquisition and the like serving as a natural product obtained by fermentation of microorganisms, can be applied to the preparation of related after-sun repair agents and has a good market application prospect.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and microorganism technology, and particularly relates to a Bacillus strain capable of producing exopolysaccharide with after-sun repairing effect and its application. Background technique [0002] According to different wavelengths, ultraviolet rays can be divided into three categories: long-wave ultraviolet (UVA) with a wavelength of 320-400nm, medium-wave ultraviolet (UVB) with a wavelength of 280-320nm, and short-wave ultraviolet (UVC) with a wavelength of 200-280nm. The ozone layer located more than ten kilometers above the earth's surface can absorb all the UVC and most of the UVB, which is the most harmful to human skin, and is a natural barrier for biological life activities on the earth. However, with the increase of air pollution year by year, the ozone layer has been severely damaged, and the ultraviolet rays radiated to the earth's surface have increased significantly, af...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A61K8/73A61Q19/00C12R1/01
CPCA61Q19/004A61K8/73C12N1/20C12P19/04C12R2001/01C12N1/205
Inventor 周文文汪旖清
Owner ZHEJIANG UNIV
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