Application of Ae.tauschii ssp.strangulata Yr4DS gene to stripe rust resistant breeding of wheat plants

Abstract

The invention discloses application of an Ae.tauschii ssp.strangulata Yr4DS gene to stripe rust resistant breeding of wheat plants. A novel wheat stripe rust resistant gene Yr4DS is cloned from Ae.tauschii ssp.strangulata for the first time, and the sequence of the gene is shown as SEQ ID NO.1, SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7, SEQ ID NO.9 or SEQ ID NO.10. The gene can effectively regulate and control the stripe rust resistance of wheat, and enriches the gene resources of wheat stripe rust resistance. The expression of the Yr4DS is found to be capable of providing a high-level stripe rustresisting function, indicating that the man-made manipulation of the Yr4DS gene can endow wheat and other wheat plants with resistance to stripe rust. By means of Yr4DS gene-based molecular mark assistant breeding, genetic transformation and gene editing, the resistance level of wheat and other wheat plants to stripe rust can be improved, and intermediate materials and production varieties capable of resisting stripe rust can be cultured and created.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to a goat grass Yr4DS gene and its application in breeding of wheat plants against stripe rust. Background technique [0002] Wheat Stripe Rust (or Yellow Rust) is a fungal disease caused by Puccinia striiformis f.sp.tritici. (Pst), which endangers wheat production worldwide. Wheat stripe rust can usually cause 0.5%-5% yield loss, and when it occurs seriously, it can cause 5%-25% yield loss, or even extinction (Chen.2005.Canadian Journal of Plant Pathology 27:314-337). China is an area where wheat stripe rust commonly occurs in the world. There have been 4 wheat stripe rust pandemics (1950, 1964, 1990 and 2002), each of which resulted in a yield loss of hundreds of millions of kilograms, and the total wheat yield loss was about 1.2 billion kilograms, of which the output loss in 1950 was about 41.4% of the total national wheat output (Chen Wanquan et al., 2013. Chinese Ag...

AI Technical Summary

Problems solved by technology

[0004] The use of disease resistance genes is an important means of controlling stripe rust. Hundreds of stripe rust resistance genes have been mapped in the genomes of wheat and its close relatives worldwide. However, due to factors such as the large and complex wheat genome, the resistance Very limited number of stripe rust genes

So far, only five genes Yr5, Yr7, Yr15, Yr18 and Yr36 have been cloned (Fu et al. .Nature Plants4:662-668; Klymiuk et al.2018.Nature Communications 9:3735), greatly limiting the effective use of stripe rust resistance genes in wheat and other crop breeding

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