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Method for improving polysaccharide content of mushroom variety 808

A technology for polysaccharide content and shiitake mushrooms, which is applied in botany equipment and methods, mushroom cultivation, horticulture, etc., can solve the problems of less synthesis of shiitake polysaccharides, limited wide-range application, and low extraction rate, so as to increase expression and increase Yield of Lentinus edodes and Effect of Optimizing Cultivation Model

Inactive Publication Date: 2019-02-19
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small amount of synthesis of lentinan, low extraction rate and high price, it limits its wide-scale application. Therefore, changing the existing cultivation mode and increasing the content of lentinan is one of the important directions to improve the economic benefits of the lentinan industry.

Method used

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  • Method for improving polysaccharide content of mushroom variety 808
  • Method for improving polysaccharide content of mushroom variety 808
  • Method for improving polysaccharide content of mushroom variety 808

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Lentinus edodes 808 cultivation bag preparation

[0022] 1.1 Preparation and identification of mother species

[0023] Choose 808, the main variety of shiitake mushrooms, inoculate it on PDA slant medium (200g of potatoes, 20g of glucose, 20g of agar), and place it at 25°C under the conditions of light intensity 0Lx for strain activation and parent seed preparation.

[0024] 1.2 Preparation of original seed

[0025] Based on the results of 1.1, after 15 days the mycelia covered the slope, start to prepare the original seed medium (sawdust 81%, bran 18%, gypsum 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, water content 65%) , using a green original seed bottle with a capacity of 500ml, 800g per bottle, sterilizing in an autoclave, and sterilizing at 121°C for 2h. After the original seed bottle was cooled for 12 hours, each female seed was inoculated in the original seed bottle, and cultivated for 40 days at 25°C under the condition of ...

Embodiment 2

[0028] Lentinus edodes color change speed and color record under different light qualities of embodiment 2

[0029] Use 6-pin black sunshade nets to build fruiting sheds in the field. Under the fruiting shed, according to the light and wind direction, five small sheds of white (CK), red, yellow, green and blue were built. Three sheds were built for each color, arranged in random blocks, and each shed was 1 meter long and wide. 0.8 meters, 0.7 meters in height, and 0.8 meters between the small sheds. At the same time, open the drainage ditch to ensure smooth drainage. The five color films are all made of 0.2mm PVC film. After the mushroom cultivation bag of Embodiment 1 is full, 20 cultivation bags are placed under each color shed. Table 1 shows the agronomic traits of mushrooms under different light quality treatments during the color change period.

[0030] Table 1 Comparison of agronomic traits of shiitake mushrooms under different light quality treatments during the color...

Embodiment 3

[0033] Example 3 Fruiting stage mushroom fruiting body character record

[0034] The experimental conditions are the same as in Example 2, and the fruiting body properties of Lentinus edodes in the fruiting stage are shown in Table 2.

[0035] Table 2 Comparison of economic traits of fruiting bodies under different light quality treatments

[0036]

[0037] It can be seen from Table 2 that different light quality treatments have a greater impact on the economic traits of shiitake mushrooms, and the fruiting time of blue light treatment is the fastest, followed by white light treatment. Under the blue light treatment, the length and diameter of the stipe of the shiitake mushrooms are appropriate, and the stipe feels firm when held in the hand. The diameter of the cap is the largest, which is 1.02 times that of the normal white light treatment. At the same time, the cap is the thickest and meets the standard of high-quality mushrooms. In addition, the yield of shiitake mushr...

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Abstract

The invention discloses a method for improving polysaccharide content of mushroom variety 808. The method includes the following specific steps: preparing raw materials, preparing mother seeds, preparing original seeds, preparing cultivation bags, culturing the cultivation bags at 25 DEG C with light intensity of 0 Lx, and placing the cultivation bags under blue light conditions for color conversion and fruiting management until hyphae are full of the bags. According to the method, the polysaccharide content can be obviously improved, the agronomic characteristics of the mushrooms are optimized, the yield of the mushrooms is improved, the activity of lignin degrading enzyme laccase and polysaccharide synthesis key enzyme UG Pase enzyme and the expression amount of correlative coding genesare improved, and the support for optimizing the existing mushroom cultivation mode, improving the planting benefits of the mushrooms and boosting the development of the mushroom processing industry is provided.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for increasing the polysaccharide content of shiitake mushroom variety 808. Background technique [0002] Mushrooms (Lentinus edodes) belong to Basidiomycetes, Agaricaceae, Pleurotaceae, also known as mushrooms, winter wild rice, flower mushrooms and so on. Its cultivation areas are mainly distributed in Henan, Fujian, Zhejiang, Anhui, Hunan, Hubei, Jiangxi, Sichuan, Guangdong, Guangxi, Hainan, Guizhou, Yunnan, Shaanxi, Gansu and other places. Lentinus edodes is an edible fungus that is popular among consumers in the world. Its production and consumption in the world are second only to Agaricus bisporus, and it is one of the important industries with strong competitiveness in my country's agricultural industrial structure. . Shiitake 808, as the main shiitake mushroom variety in my country, is the most widely cultivated, with the best benefits ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 辜运富吴海冰曾先富向泉桔李昕竺陈强赵珂张小平
Owner SICHUAN AGRI UNIV
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