Forest tree rhizosphere bacteria Bacillus paramycoides JYZ-SD5 and application thereof

A technology of JYZ-SD5 and Bacillus mycoides, applied in the field of microorganisms, can solve the problem that the comprehensive performance of PGPR is relatively less studied.

Active Publication Date: 2019-02-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The beneficial function of PGPR to plants under natural conditions is the result of the interaction of many aspects. However, most of the current research on PGPR focuses on one or some biological functions, and there are relatively few studies on the comprehensive performance of PGPR.

Method used

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  • Forest tree rhizosphere bacteria Bacillus paramycoides JYZ-SD5 and application thereof
  • Forest tree rhizosphere bacteria Bacillus paramycoides JYZ-SD5 and application thereof
  • Forest tree rhizosphere bacteria Bacillus paramycoides JYZ-SD5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The bacterium JYZ-SD5 isolated from the rhizosphere of poplar in Shandong is preserved in the China Center for Type Culture Collection with the preservation number: CCTCC NO: M2018469.

[0032] 1) Physiological and biochemical measurements of the bacterial strain JYZ-SD5 were performed referring to the "Bergery Bacteria Identification Handbook" and "Common Bacterial System Identification Handbook" to describe the colony morphology and characteristics.

[0033] The colony of the strain JYZ-SD5 on the NA medium is nearly round, with irregular edges, milky white, no protrusion in the middle of the colony, moist and shiny ( figure 1 A, B). The bacterium is short rod-shaped, without flagella, Gram-positive bacteria, and green spores can be seen in the spore staining observation, indicating that spores are produced ( figure 1 C).

[0034] Strain JYZ-SD5 was positive in VP test, methyl red test, indole test and nitric acid reduction test, and could hydrolyze starch and gelat...

Embodiment 2

[0042] Example 2 Rhizobacteria JYZ-SD5 Nitrogen Fixation, Phosphorus Solution, Potassium Solution Performance Measurement

[0043] 1) Detection of nitrogen fixation characteristics: Inoculate the tested strains in nitrogen-free medium by streaking on a plate, repeat each treatment 3 times, place them in a low-temperature constant temperature incubator (MIR-553, Japan) at 28°C, and observe the bacteria continuously growth status.

[0044] 2) Detection of organophosphate-dissolving characteristics: inoculate the tested strains in the organophosphate-dissolving medium by three-point inoculation method, repeat each treatment 3 times, place them in an incubator at 28°C, and continuously observe the density of the transparent circle around the colony. Yes or no.

[0045] 3) Determination of potassium decomposing ability: adopt the method of liquid culture to inoculate the test strain into the liquid medium for detecting potassium decomposing, repeat each treatment 3 times, place it...

Embodiment 3

[0049] Example 3 Rhizobacteria JYZ-SD5 produces IAA ability detection

[0050] 1) Qualitative detection: Pick a single colony and insert it into King's medium B, incubate in a constant temperature shaker (MultitronStandard, Switzerland) at 37°C for 24 hours, use a sterile pipette tip to draw 1mL of bacterial liquid into a sterile centrifuge tube, add 4mL of Sackowcki's After the developer was mixed quickly and fully, the color was developed at room temperature for 40 minutes in the dark, and the color change was observed.

[0051] 2) Quantitative detection: Accurately weigh 10mg of IAA, first dissolve it with a small amount of ethanol, then dilute to 100mL with distilled water (concentration is 100μg / mL), and then dilute to 0, 4, 8, 12, 16, 20, 24μg / mL respectively , take 1mL of each concentration, add 4mL color reagent respectively, keep dark at 40°C for 40min, measure OD value at OD535, and draw a standard curve. Centrifuge the cultured bacterial suspension and the blank co...

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Abstract

The invention discloses forest tree rhizosphere bacteria Bacillus paramycoide s JYZ-SD5 and application thereof. The forest tree rhizosphere bacteria are classified and named as Bacillus paramycoidesJYZ-SD5, and preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2018469. By detecting the biological characteristics of the bacteria JYZ-SD5, the bacteriahave the abilities of nitrogen fixation, phosphorus dissolution, potassium dissolution and IAA production, have an obvious promotion effect on the growth of forest trees, have certain inhibition effects on some forest tree pathogenic fungi, have high heavy metal tolerance, such as Ni2+, Gr6+ and Mn7+, and have a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a tree rhizosphere bacterium JYZ-SD5 and its application. Background technique [0002] Soil microbial communities are rich, each with different biological functions. Plant growth promoting rhizobacteria (PGPR) live in the rhizosphere and root surface of plants, and have the functions of promoting plant growth, antagonizing pathogenic microorganisms, improving plant resistance, repairing soil, and maintaining soil quality and health. For example, PGPR can rapidly reproduce in the rhizosphere of plants, fix nitrogen in the atmosphere, make plants make full use of soil nutrients, and promote better plant growth. Liu et al. screened a strain of Pseudomonas fluorescens JW-JS1 from the poplar rhizosphere, which can significantly promote the growth and nutrient element content of NL-895 poplar and black poplar seedlings. Song Fangxu et al. found that the rhizosphere bact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/205C12R2001/07
Inventor 吴小芹徐秀倩叶建仁吴天宇刘辉
Owner NANJING FORESTRY UNIV
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