Preparation method of turtle peptide

A soft-shelled turtle peptide and soft-shelled turtle technology, applied in the field of soft-shelled turtle peptide preparation, can solve the problems of complex preparation process, low proportion of polypeptide, and low enzymatic hydrolysis efficiency, and achieve the effect of simple preparation process, good biological activity, and high enzymatic hydrolysis efficiency

Inactive Publication Date: 2019-03-01
兰溪市哥特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of this preparation method is complicated, the enzymatic h

Method used

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Example Embodiment

[0018] Example 1:

[0019] The preparation method of turtle peptide includes the pretreatment of turtle, the extraction of turtle collagen, the proteolysis of turtle collagen, and the purification of turtle peptide solution. The specific steps are as follows:

[0020] 1) Pretreatment of soft-shelled soft-shelled turtle: select fresh soft-shelled turtle raw materials, discard the internal organs after slaughter, and wash it with ice water, then place the cleaned soft-shelled turtle at 0.1MPa, 120°C steam for 10 minutes, cool and drain, and put it into a meat grinder Chop it in the middle, then add distilled water 4 times the weight of the soft-shelled soft-shelled turtle meat. Use a tissue masher to smash to get the soft-shelled turtle homogenate. For use, the high-temperature and high-pressure steam treatment can kill the bacteria carried by the soft-shelled turtle and can also help the turtle protein. The effect of denaturation, while mashing the tissues can achieve uniform distri...

Example Embodiment

[0027] Example 2:

[0028] The preparation method of turtle peptide includes the pretreatment of turtle, the extraction of turtle collagen, the proteolysis of turtle collagen, and the purification of turtle peptide solution. The specific steps are as follows:

[0029] 1) Pretreatment of soft-shelled soft-shelled turtle: select fresh soft-shelled turtle raw materials, discard the internal organs after slaughter, and wash it with ice water, then place the cleaned soft-shelled turtle at 0.13MPa, steam at 122°C for 20 minutes, cool and drain, and put it into a meat grinder Minced in the medium, minced, add distilled water 4 times the weight of the soft-shelled turtle meat, mash it with a tissue masher to obtain the soft-shelled turtle homogenate, and set aside;

[0030] 2) Extraction of soft-shelled turtle protein: Put the soft-shelled turtle homogenate in a water bath and heat it at 100°C for 30 minutes with constant stirring. After the reaction, cool to room temperature and centrifuge ...

Example Embodiment

[0036] Example 3:

[0037] The preparation method of turtle peptide includes the pretreatment of turtle, the extraction of turtle collagen, the proteolysis of turtle collagen, and the purification of turtle peptide solution. The specific steps are as follows:

[0038] 1) Pretreatment of soft-shelled soft-shelled turtle: select fresh soft-shelled turtle raw materials, discard the internal organs after slaughter, and wash it with ice water, then place the cleaned soft-shelled turtle at 0.15 MPa, steam at 125 ℃ for 10 minutes, cool and drain, and put it into a meat grinder Minced in the medium, minced, add distilled water 4 times the weight of the soft-shelled turtle meat, mash it with a tissue masher to obtain the soft-shelled turtle homogenate, and set aside;

[0039] 2) Extraction of soft-shelled turtle protein: Put the soft-shelled turtle homogenate in a water bath and heat it at 100°C for 20 minutes with constant stirring. After the reaction, cool to room temperature and centrifuge...

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PUM

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Abstract

The invention discloses a preparation method of turtle peptide. The preparation method comprises the following steps: firstly placing washed turtles in high-temperature and high-pressure steam for treating, cooling and then draining, treating pounded turtle tissue homogenate under a high-temperature condition, centrifuging and then obtaining bottom sediment which is turtle protein, then mixing theturtle protein with distilled water to prepare a turtle protein solution, adding complex enzymes and enzymolysis additives for reacting, controlling time, temperature and pH of enzymolysis, carryingout enzyme deactivation after enzymolysis is ended, cooling to room temperature and centrifuging to obtain supernate which is a turtle peptide solution, decolorizing and deordorizing the turtle peptide solution, then treating the turtle peptide solution by using a micro-filtration membrane and an ultrafiltration membrane, finally carrying out rotary evaporation and concentration of filtrate, freezing at low temperature, and carrying out vacuum drying to obtain the turtle peptide. The preparation method is simple in process and high in enzymolysis efficiency; the commercialized development is facilitated; the prepared turtle peptide is high in purity, high in sensory quality, free of color, smell and impurities, high in ratio of low-molecular-weight polypeptide and excellent in biological activity, and can be conveniently absorbed and used by the body.

Description

technical field [0001] The invention relates to the technical field of soft-shelled turtle deep processing, in particular to a preparation method of soft-shelled turtle peptide. Background technique [0002] Soft-shelled turtle, also known as soft-shelled turtle and turtle, is not only delicious in meat, rich in nutrition, rich in protein, fat, calcium, iron and various vitamins, but also all parts of the body can be used as medicine, which has the functions of nourishing yin and clearing heat, nourishing qi and delaying aging It is a traditional health food raw material in my country. [0003] Soft-shelled turtle peptide is a small molecular peptide obtained by hydrolyzing soft-shelled turtle protein with complex enzymes, so as to increase the biological functions that are not found in the original food protein. Modern nutritional studies have found that most of the protein ingested by the human body is digested and absorbed in the form of oligopeptides, and the biological ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06
CPCC07K14/78C07K1/14C07K1/34C12P21/06
Inventor 李健
Owner 兰溪市哥特生物技术有限公司
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