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Preparation method of turtle peptide

A soft-shelled turtle peptide and soft-shelled turtle technology, applied in the field of soft-shelled turtle peptide preparation, can solve the problems of complex preparation process, low proportion of polypeptide, and low enzymatic hydrolysis efficiency, and achieve the effect of simple preparation process, good biological activity, and high enzymatic hydrolysis efficiency

Inactive Publication Date: 2019-03-01
兰溪市哥特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of this preparation method is complicated, the enzymatic hydrolysis efficiency is low, and the proportion of low molecular weight polypeptides is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method of soft-shelled turtle peptide includes soft-shelled turtle pretreatment, extraction of soft-shelled turtle collagen, enzymatic hydrolysis of soft-shelled turtle collagen, and purification of soft-shelled turtle peptide solution. The specific steps are as follows:

[0020] 1) Soft-shelled turtle pretreatment: select fresh raw soft-shelled turtle, discard the viscera after slaughter, and clean it with ice water, then place the cleaned soft-shelled turtle in 0.1MPa, 120°C steam for 10 minutes, cool it, drain it, and put it in a meat grinder Minced soft-shelled turtle meat, then add distilled water 4 times the weight of minced soft-shelled turtle meat, and use a tissue masher to mash soft-shelled turtle homogenate for later use. High-temperature and high-pressure steam treatment can kill the bacteria in the soft-shelled turtle and also play a role in soft-shelled turtle protein. Denaturation, while tissue mashing can realize the uniform distribution o...

Embodiment 2

[0028] The preparation method of soft-shelled turtle peptide includes soft-shelled turtle pretreatment, extraction of soft-shelled turtle collagen, enzymatic hydrolysis of soft-shelled turtle collagen, and purification of soft-shelled turtle peptide solution. The specific steps are as follows:

[0029] 1) Soft-shelled turtle pretreatment: select fresh raw soft-shelled turtle, discard the viscera after slaughter, and clean it with ice water, then place the cleaned soft-shelled turtle in 0.13MPa, 122°C steam for 20min, cool it, drain it, and put it into a meat grinder Minced soft-shelled turtle, then add distilled water 4 times the weight of minced soft-shelled turtle meat, and use a tissue masher to mash to obtain soft-shelled turtle homogenate, set aside;

[0030] 2) Extraction of soft-shelled turtle protein: put soft-shelled turtle homogenate in a water bath, heat at 100°C for 30 minutes, and keep stirring. After the reaction, cool to room temperature and centrifuge at 6000rpm...

Embodiment 3

[0037] The preparation method of soft-shelled turtle peptide includes soft-shelled turtle pretreatment, extraction of soft-shelled turtle collagen, enzymatic hydrolysis of soft-shelled turtle collagen, and purification of soft-shelled turtle peptide solution. The specific steps are as follows:

[0038] 1) Soft-shelled turtle pretreatment: select fresh raw soft-shelled turtle, discard the viscera after slaughter, and clean it with ice water, then place the cleaned soft-shelled turtle in 0.15MPa, 125°C steam for 10min, cool it, drain it, and put it into a meat grinder Minced soft-shelled turtle, then add distilled water 4 times the weight of minced soft-shelled turtle meat, and use a tissue masher to mash to obtain soft-shelled turtle homogenate, set aside;

[0039] 2) Extraction of soft-shelled turtle protein: put the soft-shelled turtle homogenate in a water bath, heat it at 100°C for 20 minutes, and keep stirring. After the reaction, cool to room temperature and centrifuge at ...

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Abstract

The invention discloses a preparation method of turtle peptide. The preparation method comprises the following steps: firstly placing washed turtles in high-temperature and high-pressure steam for treating, cooling and then draining, treating pounded turtle tissue homogenate under a high-temperature condition, centrifuging and then obtaining bottom sediment which is turtle protein, then mixing theturtle protein with distilled water to prepare a turtle protein solution, adding complex enzymes and enzymolysis additives for reacting, controlling time, temperature and pH of enzymolysis, carryingout enzyme deactivation after enzymolysis is ended, cooling to room temperature and centrifuging to obtain supernate which is a turtle peptide solution, decolorizing and deordorizing the turtle peptide solution, then treating the turtle peptide solution by using a micro-filtration membrane and an ultrafiltration membrane, finally carrying out rotary evaporation and concentration of filtrate, freezing at low temperature, and carrying out vacuum drying to obtain the turtle peptide. The preparation method is simple in process and high in enzymolysis efficiency; the commercialized development is facilitated; the prepared turtle peptide is high in purity, high in sensory quality, free of color, smell and impurities, high in ratio of low-molecular-weight polypeptide and excellent in biological activity, and can be conveniently absorbed and used by the body.

Description

technical field [0001] The invention relates to the technical field of soft-shelled turtle deep processing, in particular to a preparation method of soft-shelled turtle peptide. Background technique [0002] Soft-shelled turtle, also known as soft-shelled turtle and turtle, is not only delicious in meat, rich in nutrition, rich in protein, fat, calcium, iron and various vitamins, but also all parts of the body can be used as medicine, which has the functions of nourishing yin and clearing heat, nourishing qi and delaying aging It is a traditional health food raw material in my country. [0003] Soft-shelled turtle peptide is a small molecular peptide obtained by hydrolyzing soft-shelled turtle protein with complex enzymes, so as to increase the biological functions that are not found in the original food protein. Modern nutritional studies have found that most of the protein ingested by the human body is digested and absorbed in the form of oligopeptides, and the biological ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06
CPCC07K14/78C07K1/14C07K1/34C12P21/06
Inventor 李健
Owner 兰溪市哥特生物技术有限公司
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