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Immunochromatographic test paper for detecting MTP (methamidophos)

A technology of immunochromatographic test paper and methamidophos, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems of high technical requirements for pretreatment and operators, poor repeatability and reproducibility of analysis results, and expensive instruments and equipment, etc. Problems, to achieve the effect of protecting consumers' health, obvious economic and social benefits, and showing the image of the results

Inactive Publication Date: 2019-03-01
ZHENGZHOU INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of the biological identification method is that the sample to be tested does not need to be very pure, but the disadvantage is that the sensitivity is low and the experimental period is long
The advantage of chemical analysis is that it is economical and practical, but it cannot be accurately quantified, and the repeatability and reproducibility of the analysis results are poor.
The instrumental analysis method has the advantages of high separation, high detection efficiency and rapid analysis ability, but it has high technical requirements for sample pretreatment and operators, and the equipment is expensive, so it is not suitable for on-site rapid detection

Method used

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  • Immunochromatographic test paper for detecting MTP (methamidophos)
  • Immunochromatographic test paper for detecting MTP (methamidophos)
  • Immunochromatographic test paper for detecting MTP (methamidophos)

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Effect test

Embodiment 1

[0045] The immunochromatographic test paper of detection MTP of the present invention, with reference to Figure 1-2 , including a support and an adsorption layer fixed on the support, the adsorption layer is a sample pad 1, a binding pad 2, a chromatographic membrane 3 and an absorption pad 4 in order from the test end, and it is characterized in that the binding pad is adsorbed with Nanomaterial-labeled anti-MTP monoclonal antibody MTP-mAb; the chromatographic membrane is provided with a stealth detection blot 5 printed with a carrier protein (MTP artificial antigen) solution coupled to MTP and a goat anti- or rabbit anti-mouse Stealth control blot 6 printed by IgG antibody solution; the nanomaterials are nitrogen-doped carbon nanomaterials, carbon nanomaterials, and carbon quantum dot fluorescent nanoparticles.

[0046] The support body includes a bottom layer 7 arranged on the bottom surface of the adsorption layer and a surface layer 8 arranged on the top surface of the a...

Embodiment 2

[0057] The present embodiment detects MTP test paper, mainly includes: the preparation of MTP artificial antigen, MTP monoclonal antibody (MTP-mAb), the preparation of nitrogen-doped carbon nanomaterials (N-CDs) labeled MTP antibody and the immune system based on N-CDs labeling The steps such as the preparation of chromatography test paper, wherein, the preparation method of each product is as follows:

[0058] 1. Preparation of MTP artificial antigen (MTP-BSA)

[0059] Weigh 10mg of bovine serum albumin (BSA) and place it in a 10mL screw bottle, add 0.13mol / L NaHCO 3 The solution was made into a 10% BSA activation solution with a mass fraction of 10%, and the pH was adjusted to 7.6. Weigh 1mg of MTP, 1.073mg of N,N-dicyclohexylcarbimide and 0.598mg of N-hydroxysuccinimide, dissolve them in anhydrous tetrahydrofuran, shake at 30°C for 4h, then centrifuge at 4000rpm for 15min, and wash with anhydrous The precipitate was washed with tetrahydrofuran, and after the tetrahydrofur...

Embodiment 3

[0084] The present embodiment detects MTP test paper, mainly includes: the preparation of MTP artificial antigen, MTP monoclonal antibody (MTP-mAb), the preparation of MTP antibody labeled with carbon nanomaterials and the steps such as the preparation of immunochromatography test paper based on carbon nanomaterial label, Wherein, the preparation method of each product is as follows:

[0085] 1. Preparation of MTP artificial antigen (MTP-BSA)

[0086] With embodiment 2.

[0087] 2. Preparation of MTP-mAb

[0088] With embodiment 2.

[0089] 3. Preparation of immunochromatographic test paper based on carbon nanomaterial labeling

[0090] (1) Preparation of carbon nanomaterial TPCA

[0091] Dissolve 1.5g citric acid and 1.62g cysteamine hydrochloride in 7.5mL ultrapure water, transfer the solution to a 50mL polytetrafluoroethylene liner after fully dissolving, and then place the liner in a high-pressure reactor. React at 200°C for 3 hours. After the reaction, the product wa...

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Abstract

The invention provides immunochromatographic test paper for detecting MTP (methamidophos). The immunochromatographic test paper comprises a support body and an adsorbing layer fixed on the support body, wherein the adsorbing layer comprises a sample pad, a binding pad, a chromatography membrane and an absorption pad in sequence from a testing end, and a nanomaterial marked anti-MTP monoclonal antibody is adsorbed on the binding pad; a stealth detection imprinting printed by an MTP artificial antigen solution and a stealth control imprinting printed by a goat-anti-rat or rabbit-anti-rat IgG antibody solution are formed on the chromatography membrane; nanomaterials comprises a nitrogen-doped carbon nanomaterial, a carbon nano material and carbon quantum dot fluorescence nanoparticles. The test strip has the characteristics of being high in specificity, sensitivity and stability, good in safety, simple, fast, vivid and direct in result display, wide in applicable range, convenient to carry and quantitative. The immunochromatographic test paper has important significance in the aspects of guaranteeing food safety and protecting consumer health and has obvious economic benefit and social benefit.

Description

technical field [0001] The invention relates to an immunochromatographic test paper, in particular to an immunochromatographic test paper for detecting methamidophos (MTP). Background technique [0002] Methamidopho (MTP) is an organophosphorus compound. It is an efficient and broad-spectrum organophosphorus insecticide and acaricide. It has contact killing, stomach poisoning and certain fumigation effects, but due to its high toxicity, it is difficult to Decomposition, long residual period, a certain dose of methamidophos can cause dizziness, nausea, pulmonary edema, cerebral edema and respiratory paralysis and other symptoms, which have brought huge negative impact on human health and environmental pollution. In 2008, my country has banned its use on fruits and vegetables, but due to its broad-spectrum, high-efficiency, and low-cost characteristics, there are still quite a few growers using it in violation of the prohibition, causing methamidophos to exceed the standard an...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/577
Inventor 职爱民郭林王利平孙勇谢光辉冯冲程丽英丁勇王岚贾国超李靖靖
Owner ZHENGZHOU INST OF TECH