Preparation method for carboxymethyl chitosan-loaded porous poly(lactic-co-glycolic acid) (PLGA) nanoparticle, as well as product and application thereof

A technology of carboxymethyl chitosan and nanoparticles, applied in the field of medical biology, can solve problems such as laryngotracheal stenosis, achieve good effect, meet medicinal needs, and have a large drug load effect

Inactive Publication Date: 2019-03-08
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It should include external trauma, laryngotracheal surgery and long-term tracheal intubation, etc. However, tracheotomy and tracheal intubation are important measures to rescue patients with respiratory dysfunction. Challenging problems faced

Method used

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  • Preparation method for carboxymethyl chitosan-loaded porous poly(lactic-co-glycolic acid) (PLGA) nanoparticle, as well as product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of porous polylactic acid PLGA nanoparticle loaded with carboxymethyl chitosan, prepared according to the following steps:

[0030] Dissolve 15 mg carboxymethyl chitosan in 150 μL deionized water as the inner water phase (W1);

[0031] 150 mg PLGA (lactic acid: glycolic acid = 50:50 (w / w), carboxylic acid end groups, intrinsic viscosity: 0.18 dL / g, molecular weight: 7400 Da) was dissolved in 3.75 mL of dichloromethane as the copolymer phase (O) ;

[0032] Mix the inner aqueous phase and the copolymer phase, and use a cell breaker to ultrasonically emulsify in an ice bath for 2 minutes (ultrasonic 1s, interval 1s, power 50%, temperature 25°C) to form W1 / O primary emulsion. Add 0.75 mL of newly prepared 1.5% (w / v) ammonium bicarbonate (ABC) aqueous solution or an equal volume of deionized water to W1 / O colostrum, and use a cell breaker to ultrasonically emulsify in an ice bath for 30 s (ultrasound 1 s, interval 1 s, Power 25%, temperature 25°C) to obtain a mixtur...

Embodiment 2

[0035] A kind of porous polylactic acid PLGA nanoparticle loaded with carboxymethyl chitosan, prepared according to the following steps:

[0036] Dissolve 18.75 mg carboxymethyl chitosan in 150 μL deionized water as the inner water phase (W1);

[0037] 150 mg PLGA (lactic acid: glycolic acid = 50:50 (w / w), carboxylic acid end groups, intrinsic viscosity: 0.18 dL / g, molecular weight: 7400 Da) was dissolved in 3.75 mL of dichloromethane as the copolymer phase (O) .

[0038] Mix the inner aqueous phase and the copolymer phase, and use a cell breaker to ultrasonically emulsify in an ice bath for 2 minutes (ultrasonic 1s, interval 1s, power 50%, temperature 25°C) to form W1 / O primary emulsion. Add 0.75 mL of newly prepared 1.5% (w / v) ammonium bicarbonate (ABC) aqueous solution or an equal volume of deionized water to W1 / O colostrum, and use a cell breaker to ultrasonically emulsify in an ice bath for 30 s (ultrasound 1 s, interval 1 s, Power 25%, temperature 25°C).

[0039] Unde...

Embodiment 3

[0041] A kind of porous polylactic acid PLGA nanoparticle loaded with carboxymethyl chitosan, prepared according to the following steps:

[0042] Dissolve 22.5 mg carboxymethyl chitosan in 150 μL deionized water as the inner water phase (W1);

[0043] 150 mg PLGA (lactic acid: glycolic acid = 50:50 (w / w), carboxylic acid end groups, intrinsic viscosity: 0.18 dL / g, molecular weight: 7400 Da) was dissolved in 3.75 mL of dichloromethane as the copolymer phase (O) .

[0044] Mix the inner aqueous phase and the copolymer phase, and use a cell breaker to ultrasonically emulsify in an ice bath for 2 minutes (ultrasonic 1s, interval 1s, power 50%, temperature 25°C) to form W1 / O primary emulsion. Add 0.75 mL of newly prepared 1.5% (w / v) ammonium bicarbonate (ABC) aqueous solution or an equal volume of deionized water to W1 / O colostrum, and use a cell breaker to ultrasonically emulsify in an ice bath for 30 s (ultrasound 1 s, interval 1 s, Power 25%, temperature 25°C).

[0045] Under...

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Abstract

The invention relates to a preparation method for a carboxymethyl chitosan-loaded porous poly(lactic-co-glycolic acid) (PLGA) nanoparticle, a product and application thereof. The preparation method comprises the following steps: dissolving carboxymethyl chitosan in deionized water and taking the mixture as an inner water phase; dissolving PLGA in dichloromethane and taking the mixture as a copolymer phase; mixing the inner water phase with the copolymer phase, and performing ice-bath ultrasonic emulsification by a cell disruption instrument to form a primary emulsion; adding ammonium bicarbonate aqueous solution or isopyknic deionized water into the primary emulsion and performing ultrasonic emulsification; forming a compound emulsion under a stirring condition of a homogenizer, and removing dichloromethane; and centrifuging for freeze-drying to obtain a dry carboxymethyl chitosan PLGA nanoparticle. The porous PLGA nanoparticle is adopted by the invention and has a larger drug capacityand a better effect.

Description

technical field [0001] The invention relates to a preparation method of an inhalation spray with anti-throat scar generation, in particular to a preparation method of porous polylactic acid PLGA nanoparticles loaded with carboxymethyl chitosan and its product and application. The invention belongs to the field of medical biology. Background technique [0002] Laryngeal and tracheal cicatricial stenosis refers to the narrowing of the airway due to scar hyperplasia in the larynx or tracheal cavity, resulting in dysfunction of vocalization and / or breathing. Trauma or lesions of the larynx and trachea caused by various reasons lead to cartilage damage, mucosal rupture or hematoma formation, followed by proliferation and repair of fibrous connective tissue and scar tissue, and finally tracheal stenosis. It should include external trauma, laryngotracheal surgery and long-term tracheal intubation, etc. However, tracheotomy and tracheal intubation are important measures to rescue p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K47/34A61K9/72A61K9/14A61K31/722A61P11/04
CPCA61K9/0073A61K9/14A61K9/5153A61K31/722A61P11/04
Inventor 何丹农王萍周如鑫朱君陈益金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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