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Mineralized bone tissue engineering scaffold and preparation method thereof

A bone tissue engineering and skeleton technology, applied in the field of mineralized composite bone tissue engineering scaffolds and their preparation, can solve problems such as the adverse effect of growth factor activity, achieve excellent mineralization effect, fast mineralization, reduce treatment costs and potential effect of risk

Active Publication Date: 2019-03-08
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the growth factors are embedded in the scaffold, the degradation of the scaffold material is not completely controllable; if the growth factor is immobilized on the scaffold material, the conformation of the growth factor will change, so it will inevitably have adverse effects on the activity of the growth factor. influences

Method used

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  • Mineralized bone tissue engineering scaffold and preparation method thereof
  • Mineralized bone tissue engineering scaffold and preparation method thereof
  • Mineralized bone tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of PCL / Zwitterionic Hydrogel Composite Scaffold

[0024] Dissolve 0.5 mmol of 3-(2-methacryloyloxyethyldimethylamino)propanesulfonate (SBMA) in 1.75 mL of phosphate-buffered saline (PBS), and add 50 μL of azo initiator VA-086 (10 mg / mL) and 25 μL cross-linking agent polyethylene glycol diacrylate to form a sulfonamide zwitterionic hydrogel prepolymer solution. Polycaprolactone (PCL) polymer was dissolved in 1,4-dioxane, and a porous polycaprolactone scaffold was prepared by freeze-drying; 50 μL of the sulfonamide prepared in this example was added dropwise on the surface of the porous PCL scaffold. Ionic hydrogel pre-polymerization solution, after the pre-polymerization solution completely infiltrates the inside of the stent, carry out ultraviolet crosslinking, after irradiating the front and back sides for 5 minutes, soak in a sterile phosphate buffer solution at 37°C for 12 hours, freeze-dry to remove water A PCL / zwitterionic hydrogel composite scaffo...

Embodiment 2

[0028] (1) Preparation of PLGA / Zwitterionic Hydrogel Composite Scaffold

[0029] Dissolve 1 mmol of 3-(2-methacryloyloxyethyldimethylamino)propanesulfonate (SBMA) in 3.5 mL of phosphate-buffered saline (PBS), and add 100 μL of azo initiator VA-086 ( 10 mg / mL) and 50 μL cross-linking agent polyethylene glycol diacrylate to form a sulfonium ammonium zwitterionic hydrogel prepolymer solution. Dissolve polylactic-co-glycolic acid (PLGA) in 1,4-dioxane, and prepare a porous polycaprolactone scaffold by freeze-drying; add 150 μL of the sulfonamide prepared in this example dropwise on the surface of the porous PLGA scaffold Zwitterionic hydrogel pre-polymerization solution, after the pre-polymerization solution completely infiltrates the inside of the scaffold, carry out ultraviolet crosslinking, after irradiating the front and back sides for 12 minutes, soak in 37 ℃ sterile phosphate buffer solution for 12 hours, freeze-dry to remove water After that, a PLGA / zwitterionic hydrogel com...

Embodiment 3

[0033] (1) Preparation of PLA / Zwitterionic Hydrogel Composite Scaffold

[0034] Dissolve 7 mmol of 3-[[2-(methacryloyloxy)ethyl]dimethylammonium]propionate (CBMA) in 9 mL of phosphate-buffered saline (PBS), and add 300 μL of azo initiator VA- 086 (10 mg / mL) and 70 μL cross-linking agent polyethylene glycol diacrylate to form a carboxyammonium zwitterionic hydrogel prepolymer solution. Polylactic acid (PLA) polymer was dissolved in 1,4-dioxane, and a porous polycaprolactone scaffold was prepared by freeze-drying; 200 μL of the carboxylamine zwitterion prepared in this example was added dropwise on the surface of the porous PLA scaffold Hydrogel pre-polymerization solution, after the liquid completely infiltrates the inside of the scaffold, carry out ultraviolet cross-linking, after irradiating the front and back sides for 15 minutes, soak in 37 ℃ sterile phosphate buffer solution for 12 hours, and freeze-dry to remove water to obtain amphoteric The PLA / zwitterionic hydrogel co...

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Abstract

The invention discloses a mineralized bone tissue engineering scaffold. The mineralized bone tissue engineering scaffold comprises a porous polyester / amphiprotic ionized hydrogel composite scaffold and a calcium-phosphate mineralization material formed in the porous polyester / amphiprotic ionized hydrogel composite scaffold in situ, wherein the porous polyester / amphiprotic ionized hydrogel composite scaffold comprises a porous polyester bone scaffold and amphiprotic ionized hydrogel compounded in the porous polyester bone scaffold. The invention also discloses a preparation method of the mineralized bone tissue engineering scaffold. The composite scaffold is capable of carrying out efficient sustained release of bone growth factors, giving efficient bone conduction and bone induction performance to the surface of the overall scaffold, accelerating functional repair of bone defects under ultralow dosage of rhBMP-2 and improving the biological safety performance of the scaffold material.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a mineralized composite bone tissue engineering scaffold capable of efficiently and slowly releasing growth factors and a preparation method thereof. Background technique [0002] Worldwide, bone defects caused by trauma, tumors, diseases, and infections afflict numerous patients every year. Currently, more than 2,000,000 patients worldwide receive bone grafting operations every year. Autologous bone and allogeneic bone are the main means for bone defect repair in clinical practice. However, due to the limited source of autologous bone and the potential defects of immune rejection in allograft bone, it is of great scientific and social significance to seek alternative high-efficiency bone repair materials. [0003] Adding exogenous growth factors such as BMP-2 to artificial bone repair materials is a clinically proven method to accelerate the repair of bone defec...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/12A61L27/54A61L27/52A61L27/56A61L27/58
CPCA61L27/12A61L27/18A61L27/52A61L27/54A61L27/56A61L27/58A61L2430/02A61L2300/602A61L2300/414A61L2300/412C08L67/04
Inventor 沈健刘平生刘沛铭彭婉刘莉
Owner NANJING NORMAL UNIVERSITY
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