Method for preparing degreased soybean meal iron storage polypeptide by protease and application

A protease and soybean meal technology, which is applied in the field of preparing defatted soybean meal iron storage polypeptides, can solve the problems of affecting the protein stability of iron absorption, affecting the effect of iron supplementation, accelerating the release of iron ions, etc., achieving broad development potential and application prospects, and improving biological Utilization, the effect of improving utilization

Active Publication Date: 2019-03-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the application of ferritin as an iron supplement has the following disadvantages: First, the large molecular conformation of ferritin is easily affected by processing conditions such as high temperature and pH, which can easily lead to protein aggregation, decrease solubility and stability, and affect the Protein stability and iron absorption effect; secondly, ferritin is extremely unstable in the gastrointestinal tract, and the complex gastrointestinal environment (such as digestive enzymes, low pH, food components) promotes protein degradation, accelerates the release of iron ions, and affects the Effect of iron supplementation
[0005] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • Method for preparing degreased soybean meal iron storage polypeptide by protease and application
  • Method for preparing degreased soybean meal iron storage polypeptide by protease and application
  • Method for preparing degreased soybean meal iron storage polypeptide by protease and application

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Embodiment 1

[0034] A method for preparing defatted soybean meal iron-storing polypeptide by using protease, the steps are as follows:

[0035] Using defatted soybean meal as raw material, the ferritin is extracted and separated by water extraction, the iron-containing polypeptide components are obtained by proteolysis technology, and the iron-storing polypeptide with high iron content is obtained by further applying the hierarchical ultrafiltration technology.

[0036] Preferably, the specific steps are as follows:

[0037] (1) Utilize defatted soybean meal as raw material, soak soybean meal with 0.05-0.10M phosphate buffer solution, the pH of the phosphate buffer solution is 7.0, and it contains polyvinylpyrrolidone with a mass percentage of 1%, phosphate buffer solution: soybean meal The mass ratio is 10-15:1, the soaking time is 6-8 hours, and then homogenized with an internal cutting homogenizer for 2-3 minutes to obtain crude protein extract;

[0038] (2) Add MgCl with a concentrati...

Embodiment 2

[0049] A method for preparing defatted soybean meal iron-storing polypeptide by using protease, the steps are as follows:

[0050] (1) Preparation of soybean meal soybean ferritin

[0051] Select the defatted soybean meal as raw material, soak the soybean meal in distilled water at 4°C overnight, add 2 times the volume of buffer solution (containing 0.05M phosphate buffer, pH 7.0, and 1% polyvinylpyrrolidone by mass percentage), Soak for 7 hours, and homogenize for 2 minutes with an internal cutting homogenizer to obtain a crude protein extract. The crude protein extract can also be filtered with gauze to remove bean dregs and take the supernatant.

[0052] A concentration of 50 mM MgCl was added to the crude extract 2 After the crystals are dissolved, immediately centrifuge at 4800g for 5min, discard the precipitate, and after the supernatant is allowed to stand for 20min, add trisodium citrate crystals with a concentration of 70mM and let stand overnight. liquid. Add 1.5 ...

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Abstract

The invention relates to a method for preparing a degreased soybean meal iron storage polypeptide by protease. The method includes the steps: taking degreased soybean meal as a raw material; extracting and separating ferroprotein; acquiring iron-containing polypeptide components with molecular weight of 2.0-3.0kDa by the aid of a proteolysis technique; preparing the iron storage polypeptide with an iron component by the aid of an ultra-filtration technology. According to the method, a novel iron supplement source based on the polypeptide is developed, a polypeptide structure and iron content are researched and illuminated, and reference is provided for development of future iron supplement products. The iron storage polypeptide prepared by the method and development of related products ofthe polypeptide have wide development potential and application prospects in the industries such as food additives, feed and health food, and the method explores a new path for utilization of soybeanmeal resources.

Description

technical field [0001] The invention belongs to the field of food technology, in particular to a method and application for preparing defatted soybean meal iron-storing polypeptide by using protease. Background technique [0002] Iron deficiency is a class of diseases that seriously affect human health. Statistics from the World Health Organization in 2016 show that 2 billion people around the world suffer from iron deficiency, which is manifested in symptoms such as listlessness, anorexia, growth retardation, frequent dizziness, inability to concentrate, and poor understanding and memory. The iron supplement preparations currently used in the market are mainly divalent non-heme iron, such as inorganic ferrous (FeSO 4 ) and organic acid ferrous salts (ferrous gluconate, etc.). However, due to the low iron absorption rate of these iron supplements, the effect of iron supplementation is not ideal, and even have certain side effects, resulting in iron poisoning symptoms. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/16C07K1/34C07K1/36C12P21/06A23L33/18A23K20/147A23L29/00
CPCA23K20/147A23L29/045A23L33/18C12P21/06C07K1/14C07K1/16C07K1/34C07K1/36A23V2002/00A23V2200/30A23V2250/55
Inventor 杨瑞孟德梅周中凯
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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