A kind of pathological tissue transparent dewaxing liquid and preparation method thereof

Abstract

The present invention relates to a novel pathological tissue transparent dewaxing liquid and a preparation method thereof. The novel pathological tissue transparent dewaxing liquid comprises the following components in parts by mass: medium and long-chain saturated fatty acid ester: 85-100 parts, propylene glycol Ethers: 0‑25 parts. It also includes the following plant essential oils in parts by mass: 0-1 part. The medium-long-chain saturated fatty acid ester is obtained by esterifying medium-long-chain acid with not less than 8 C atoms and straight-chain alcohol with 2-8 carbon atoms. This new type of transparent dewaxing solution for pathological tissue can be miscible with ethanol and other dehydrating agents, paraffin and other fillers, and has low toxicity and mild effect. The tissue section stained sample will not damage the tissue cell structure, other components in the tissue cells, and tissue antigens. It meets the requirements of clinical pathological diagnosis and research institutes for high-quality stained slides, and also meets the requirements of laboratory environmental protection.

Description

technical field [0001] The invention relates to the technical field of pathological tissue sections, in particular to a transparent dewaxing liquid for pathological tissues and a preparation method thereof. Background technique [0002] Paraffin section is the most commonly used sectioning technique in qualitative analysis such as observation of tissue morphology and immunohistochemistry, and is widely used in clinical pathological diagnosis and experimental research. In the process of pathological tissue processing, after the tissue is fixed and dehydrated, it needs to be treated with a solvent that can be mixed with ethanol and dissolve paraffin, so that the paraffin can be immersed in the tissue block through its mediation. During this process, the water in the tissue is replaced by the solvent, making its refractive index close to that of tissue protein, so as to obtain a transparent tissue block. After the tissue is embedded in paraffin, it is made into a wax block. Af...

AI Technical Summary

Problems solved by technology

Although xylene has good dewaxing and transparent effects, there are the following defects: (1) xylene belongs to neurotoxins with a strong pungent smell, which paralyzes the central nervous system and autonomic nerves, and stimulates mucous membranes, which is harmful to the physiology of long-term contact personnel. The system has selective special damage; (2) Long-term use can also cause symptoms such as low immune function, anemia, dermatitis, eczema and dry skin, which will seriously endanger the health of pathological staff and cause certain pollution to the environment

(3) During the process of pathological tissue processing, the use of xylene can easily shrink and harden the pathological tissue

However, this application uses too many components, which may cause tissue damage while the operation is complicated

Images