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Near infrared detection method for procyanidine content of grape pips

A technology for anthocyanin content and near-infrared detection, which is used in measurement devices, preparation of samples for testing, and material analysis by optical means. The process is complicated and other problems, so as to achieve the effect of fast and accurate detection, simplifying the sample processing process, and simplifying the pretreatment.

Active Publication Date: 2019-03-15
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the HPLC method can accurately detect multiple single substances, but the pretreatment process is complicated and the analysis time is long; the vanillin-sulfuric acid method will release a large amount of heat when adding sulfuric acid, causing the decomposition of proanthocyanidins, so that the detection results are low; iron salt catalysis The colorimetric method takes a long time to develop and is easy to operate, but the reproducibility is poor and needs further improvement
[0004] Due to the complex pre-processing, long detection time, and expensive detection equipment, it is difficult for the above three detection methods to achieve rapid and accurate detection of large samples.

Method used

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  • Near infrared detection method for procyanidine content of grape pips
  • Near infrared detection method for procyanidine content of grape pips
  • Near infrared detection method for procyanidine content of grape pips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Establishing a standard curve of a quadratic equation with hydrochloric acid-n-butanol method

[0053] (1) Preparation of standard solution: Weigh 0.0600g (accurate to 0.0001g) of calibrated grape seed extract powder sample into a 50mL brown volumetric flask, add 2 / 3 volume of methanol, and dissolve by ultrasound. After cooling to 25°C, dilute to volume with methanol and shake well. Pipette 0, 1, 2, 3, 4, and 5 mL of the solution into a 50 mL volumetric flask, add methanol to the mark, and shake well for use.

[0054] (2) Preparation of 2% ferric ammonium sulfate solution: accurately weigh 2.00g (accurate to 0.0001g) of ferric ammonium sulfate in a 100mL volumetric flask, add about 50mL purified water, dissolve by ultrasound, and draw 16.7mL concentrated hydrochloric acid (12M) Add, let cool to room temperature, make the volume with purified water, shake well and set aside.

[0055] (3) Preparation of 5% hydrochloric acid-n-butanol solution: add about 2 / 3 volume of...

Embodiment 2

[0058] Example 2 Near-infrared detection method of proanthocyanidin content in grape seeds

[0059] A. Pretreatment of grape seed samples

[0060] Pretreatment 1: The Cabernet Sauvignon grape seed sample is manually removed from gravel, soil, grape skin residue and other impurities, crushed to 90% or more, can pass through a 40-mesh sieve, and dried at 60°C to a moisture content of ≤5% ,spare;

[0061] Pretreatment 2: Manually remove impurities from the sample of Cabernet Sauvignon grape seeds. Use a tablet press to compress the crushed grape seed materials. The press pressure of the tablet press is 6000kPa, the diameter of the tablet is 2cm, and the thickness is 1cm. After the film is dried at 60°C until the moisture content is ≤5%, use it for later use

[0062] B. Determination of proanthocyanidin content in grape seed samples by hydrochloric acid-n-butanol method

[0063] Take 10g of the grape seed sample after pretreatment 1, add 200mL of 40% ethanol solution, extract for 30min at...

Embodiment 3～7

[0071] The method steps in Examples 3 to 4 are the same as those in Example 1, except that step (1) in Example 3 is to pipette 0, 2, and 4 mL of the solution into a 50 mL volumetric flask, and add methanol to the mark to obtain The standard curve of a quadratic equation in one variable, that is, y = 0.0913x 2 +0.1583x-0.0001, R 2 =0.9999; Step (1) in Example 4 is to pipette 0, 1, 2, 3, 4, 5, 6, and 7mL of the solution into a 50mL volumetric flask, add methanol to the mark, and obtain the standard curve of the quadratic equation in one element , Ie y=0.1064x 2 +0.1478x+0.0004, R 2 = 0.9999.

[0072] The method steps in Examples 5-7 are the same as those in Example 2, except for the selection of parameters, as shown in Table 1 below.

[0073] Table 1

[0074]

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Abstract

The invention discloses a near infrared detection method for procyanidine content of grape pips and belongs to the field of a detection method. The method comprises the following steps: pretreating grape pip samples; measuring the procyanidine content of the grape pip samples by a hydrochloric acid-normal butanol method; scanning by a near infrared spectroscopy to acquire spectroscopic data of thegrape pip samples; establishing a near infrared spectral model of the procyanidine content by combining a spectroscopic data pretreatment method with a stoichiometric modeling method; and taking theto-be-measured grape pip samples, acquiring spectral information data of the to-be-measured grape pip samples and substituting into the near infrared spectral model to acquire the procyanidine contentof the grape pip samples. By the method, the sample treatment process can be simplified; the detection time can be shortened; and rapid and accurate detection of the procyanidine content of the grapepip samples can be realized.

Description

Technical field [0001] The invention relates to a method for detecting plant extracts, in particular to a method for rapidly detecting proanthocyanidins in grape seeds, and belongs to the field of detection methods. Background technique [0002] Proanthocyanidin (Proanthocyanidin, PC) is a kind of bioflavonoids, a general term for polymers made by polymerizing different amounts of flavan-3-ol or flavan-3,4-diol. Proanthocyanidins have strong antioxidant properties, can remove oxygen free radicals in mammals, reduce the level of oxidative stress in the body, and then play a role in improving blood pressure and preventing atherosclerosis. In addition, proanthocyanidins also have the effects of inhibiting tumors, improving the immune level of the body, and protecting the liver. They have very good development prospects in medical and health care. [0003] Grape seed is one of the important sources of proanthocyanidins as waste residues in the production of table grapes, canned grapes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563G01N1/28
CPCG01N1/28G01N21/3563G01N21/359G01N2021/3572
Inventor 陶晓青霍书香杨清山程远欣连运河
Owner CHENGUANG BIOTECH GRP CO LTD
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