Seed Production and Cultivation Methods of Matsutake
A cultivation method and technology of matsutake, applied in the field of matsutake seed production and cultivation, can solve the problems of low fruiting rate, low fruiting rate of matsutake, easy death of mycelia, etc., achieve high fruiting rate, completely consistent taste, and not easy The effect of death
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preparation example Construction
[0056] The preparation method of the original seed medium or cultivar medium is as follows: mix water, tree roots, matsutake mushroom nest mud, sugar, white wine, vitamin B2 and vitamin B6, boil for 1-2 hours, and use a 1-1.5mm sieve to Filtrate, take the filtrate, add triacontanol, soybean flour and white wine, heat to boil again, add cornmeal and yellow mud after cooling, mix well, and high-pressure sterilize to obtain the original raw material / cultivation medium; among them, the white wine is divided into two times Adding, the weight ratio of adding liquor for the first time to adding liquor for the second time is 1~2:1. Preferably, the granules of the culture medium are 2-8mm, and when the granules are too fine, the growth of mycelia will be affected.
[0057] Preferably: the raw material stock / cultivator culture medium is steamed for 1 to 2 hours to obtain the clinker stock / cultivator culture medium.
[0058] The original seed / cultivar culture medium used in the present ...
Embodiment 1
[0070] Embodiment 1 prepares the culture medium that spore separation method uses
[0071] Mother seed medium:
[0072] 1. Take raw materials by weight: 1200 parts of water, 50 parts of tree roots, 1000 parts of cornmeal, 25 parts of soybean flour, 25 parts of matsutake nest mud, 4 parts of glucose, 10 parts of matsutake wine soaked in wine, 0.005 parts of vitamin B2, 100 parts of agar;
[0073] 2. Mix water, cornmeal, tree roots, matsutake mushroom nest mud, soybean noodles, glucose, marinated matsutake juice wine and vitamin B2, boil for 1-2 hours, filter; mix the filtrate with agar, heat to melt, mix well, and cool , to obtain the mother seed culture medium;
[0074] When the roots used as raw materials are pine roots, the prepared mother seed medium is denoted as MS, and when the adopted roots are the roots of A. chinensis, the prepared mother seed culture medium is denoted as MQ.
[0075] stock and cultivar media
[0076] 1. Raw materials by weight: 1000 parts of wate...
Embodiment 2
[0079] Embodiment 2 prepares the original seed / cultivar culture medium used by the spore tissue separation method
[0080] Raw materials by weight: 1000 parts of water, 50 parts of pine roots, 50 parts of green bark roots, 30 parts of white sugar, 50 parts of matsutake mushroom nest mud, 120 parts of yellow mud, 21 parts of white wine, 0.005 parts of vitamin B2, triacane 0.14 parts of alcohol, 25 parts of soybean flour, 1000 parts of corn flour;
[0081] Mix water, pine root, green bark root, matsutake mushroom nest mud, sugar, white wine and vitamin B2, boil for 1-2 hours, filter through a 1-1.5mm sieve, take the filtrate, add triacontanol, soybean flour and white wine, heat to boil again, add cornmeal and yellow mud after cooling and mix well, the particle size of the medium is 2-8mm; high-pressure sterilization to obtain the original seed / cultivation medium of raw material; among them, white wine is added in two times, the first time The weight ratio of adding white wine a...
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