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Tissue Culture Rapid Propagation Method of Jinghong Zakura

A tissue culture and rapid technology, applied in the field of plant tissue culture, can solve the problems of short stem segments, difficult elongation, high induced differentiation rate, low pollution rate, etc. Good elongation effect

Active Publication Date: 2021-09-21
河南星华农林科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a tissue culture rapid propagation method of Jinghong Zaoying, which mainly overcomes the problems of browning, short stem nodes, difficult elongation, slow growth, and difficult rooting in tissue culture. The method realizes Jinghong Zaoying Tissue culture rapid propagation, low pollution rate, low browning rate, high induction differentiation rate, rapid proliferation, good elongation of new shoots, high proliferation coefficient, strong root system after rooting culture, good toughness, large number of roots, and rooting rate High, the survival rate of hardened seedlings is significantly improved after transplanting

Method used

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  • Tissue Culture Rapid Propagation Method of Jinghong Zakura
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  • Tissue Culture Rapid Propagation Method of Jinghong Zakura

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preparation example Construction

[0062] Preferably, the GO gel mother liquor is mainly prepared by the following steps:

[0063] Add graphite powder to oxidant A at 80-90°C to react for 3-4 hours, separate and dry to obtain reactant A; then add oxidant B to reactant A under ice bath conditions for mixing, and the mixture is kept at 40- React at 50°C for 12-24 hours, add water to continue mixing for 1-10 hours, and let stand; discard the supernatant, the lower suspension is the GO gel mother liquor; the oxidant A is Mn 3 o 4 and [Co(NH 3 ) 6 ] Cl 3 Dissolved in concentrated sulfuric acid at 100-120°C, the oxidant B is KMnO 4 Dissolved in concentrated sulfuric acid obtained.

[0064] Preferably, the GO gel solution is prepared by the following method:

[0065] (1) Mn 3 o 4 and [Co(NH 3 ) 6 ] Cl 3Dissolve in concentrated sulfuric acid at 100-120°C to obtain oxidant A;

[0066] (2) adding the graphite powder into oxidant A at 80-90°C to react for 3-4 hours, separating and drying to obtain reactant A; ...

Embodiment 1

[0122] A tissue culture rapid propagation method of Beijing red early cherry blossoms, comprising the following steps:

[0123] (1) Explant material:

[0124] Collect the explants, remove the leaves, keep the 5mm petiole and the tender part of the top of the branch, and cut the branch to 2cm, containing at least 1 axillary bud.

[0125] (2) explant disinfection:

[0126] Shake and wash with saturated soapy water for 10 minutes, then rinse with tap water. The pretreated material is soaked in 70% alcohol for 10 seconds in an ultra-clean workbench, 0.6% H 2 o 2 Soak and shake in the solution for 15min, 0.1% HgCl 2 Soak in the solution and shake for 4 minutes, rinse with sterile water for 5 times, 3 minutes each time, and finally dry the water with sterile filter paper.

[0127] (3) Start training:

[0128] Soak the sterilized stems in 50mg / L ascorbic acid + 100mg / L citric acid autoclaving solution for half an hour to reduce browning, then transfer to the starting medium, the...

Embodiment 2-5

[0138] The difference between embodiment 2-5 and embodiment 1 is that the starting medium in step (3) is different, specifically as follows:

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Abstract

The invention discloses a tissue culture and rapid propagation method of Jinghong Zazakura, which relates to the technical field of plant tissue culture. The tissue culture rapid propagation method of Jinghong Zaoying includes providing explants, explant disinfection, starting culture, proliferation culture, strong seedling culture, rooting culture and seedling hardening and transplanting. Through targeted improvement of these steps, the realization of The effective tissue culture and rapid propagation of Jinghong Zaoying, adopting the method of the present invention can significantly reduce the pollution rate and browning rate of sprouts after cultivation after the disinfection of Jinghong Zaoying explants and the initiation of cultivation, and the induction differentiation rate after proliferation and cultivation High, rapid proliferation, good elongation of new shoots formed, high multiplication coefficient, strong root system, good toughness, large number of roots after rooting culture, high rooting rate, and significantly improved survival rate after hardening and transplanting. The method of the invention is simple and easy to implement, and provides technical support for the genetic transformation and molecular breeding of Jinghong Zakura.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of Jinghong Zazakura. Background technique [0002] Cerasus serrulata 'Jing hong zao ying', is one of the few large red double-flowered early cherry varieties that can withstand minus 20 degrees. It is a deciduous tree under the genus Sakura in the family Rosaceae. It has high ornamental value and adaptability The advantages of high strength and strong resistance are of great development value. [0003] Traditional cherry blossom propagation methods mainly include seeding, branching, grafting, and high-altitude layering. Most cherry blossom species are not easy to take root from cuttings. These propagation methods are slow to grow seedlings and difficult to mass produce. With the development of tissue culture technology, tissue culture has become a new way for rapid propagation and factory production of cherry blossoms. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孔德政吕林芳刘艺平刘二分李文玲范跃峰陈旭彬冯艳王阿丽张达张艳张缓缓
Owner 河南星华农林科技有限公司