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Compound microorganism bacterium agent as well as preparation method and application thereof

A compound microbial inoculum, compound microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that the speed and efficiency have not yet reached the requirements of large-scale engineering applications, the treatment effect is not ideal, and engineering applications problems such as limited value, to achieve the effects of fast reproduction, low price and simple preparation

Pending Publication Date: 2019-03-26
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The speed and efficiency of physical and chemical methods have not yet met the requirements of large-scale application in engineering, and the cost is also high, so the application value of actual engineering is limited
And because antibacterial substances are bacteriostatic at low concentrations and bactericidal at high concentrations, when the initial concentration of berberine hydrochloride changes greatly, the treatment effect is not ideal.

Method used

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  • Compound microorganism bacterium agent as well as preparation method and application thereof
  • Compound microorganism bacterium agent as well as preparation method and application thereof
  • Compound microorganism bacterium agent as well as preparation method and application thereof

Examples

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preparation example Construction

[0032] The present invention also provides a preparation method for the above-mentioned composite microbial bacterial agent, comprising the following steps: separately fermenting Bacillus circulans, Bacillus spp., Stenotrophomonas maltophilia, Bacillus cereus, Sphingomonas spp. and Paleobacterium pallidum for 24 hours After mixing; centrifuge and filter the mixed composite microorganisms to collect the bacterial cells to obtain the composite microorganism bacterial agent.

[0033] In the preparation method of the present invention, the fermentation medium preferably includes components at the following concentrations: 3-8 g / L of yeast extract powder, 6-16 g / L of tryptone and 6-16 g / L of sodium chloride. The fermentation medium of the present invention contains yeast extract powder, and the concentration of the yeast extract powder is preferably 4-6 g / L, more preferably 5 g / L. The fermentation medium of the present invention contains tryptone, and the concentration of the trypt...

Embodiment 1

[0038] Activated sludge collected from the sewage treatment plant of berberine antibiotic pharmaceutical wastewater, obtained after domestication, separation and purification: Pallidum. Add 6 bacteria into the liquid culture medium with the formula of 0.5% yeast extract powder, 1% tryptone and 1% sodium chloride respectively. It is fermented and produced at a temperature of 25°C, a pH of 7, and a dissolved oxygen (DO) of 2-4 mg / L. Control the water content of the 6 bacteria within 2%, then add 1% glycerin as a protective agent, and then centrifuge at 5000rpm to collect the bacteria for cryopreservation. At this time, the microbial mass can reach 1.0×10 8 cells / g. Before compounding the microbial agent, mix 6 bacteria according to the ratio of biomass 1:1:1:1:1:1, and set aside.

[0039] The composite microbial inoculum prepared in above-mentioned embodiment 1 is tested:

[0040] Experiment 1

[0041] The berberine standard product was prepared into a berberine solution wi...

Embodiment 2

[0062] Activated sludge collected from the sewage treatment plant of berberine antibiotic pharmaceutical wastewater, obtained after domestication, separation and purification: Pallidum. Add 6 bacteria into the liquid culture medium with the formula of 0.5% yeast extract powder, 1% tryptone and 1% sodium chloride respectively. It is fermented and produced at a temperature of 25°C, a pH of 7, and a dissolved oxygen (DO) of 2-4 mg / L. Control the water content of the 6 bacteria within 2%, then add 1% glycerin as a protective agent, and then centrifuge at 5000rpm to collect the bacteria for cryopreservation. At this time, the microbial mass can reach 1.0×10 8 cells / g. Before compounding the microbial agent, mix 6 bacteria according to the ratio of biomass 1:1:1:1:1:1, and set aside.

[0063] The single bacterium that above-mentioned embodiment 1 and embodiment 2 prepare and experiment with composite microbial inoculum:

[0064] A berberine solution with a concentration of 30 mg...

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Abstract

The invention provides a compound microorganism bacterium agent as well as a preparation method and application thereof and relates to the technical field of microorganism bacterium agents. An activecomponent of the compound microorganism bacterium agent comprises a compound microorganism; the compound microorganism comprises: bacillus circulans, bacillus, stenotrophomonas maltophilia, bacillus cereus, sphingomonas and ochrobactrum; the total amount of microorganisms in the compound microorganism bacterium agent is not lower than 1*10<7> cfu / mL. The compound microorganism bacterium agent canbe used for treating berberine production wastewater; the adding amount of the compound microorganism bacterium agent is 2 percent, the removal rate of berberine is 100 percent, the COD (Chemical Oxygen Demand) removal rate is 75 to 94 percent, the TOC (Total Organic Carbon) removal rate is 83 to 95 percent, the NH3-N removal rate is 89 to 92 percent and the TP (Total Phosphorus) removal rate is 73 to 100 percent.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculum, and in particular relates to a composite microbial inoculum and its preparation method and application. Background technique [0002] Berberine (berberine), also known as berberine hydrochloride, molecular formula C 20 h 18 NO 4 , Molecular weight 336.37, yellow crystalline powder, odorless, extremely bitter taste, is a natural isoquinoline alkaloid with various pharmacological and biological effects, and is a broad-spectrum antibacterial drug widely used at present. The production process of berberine involves the use of various aromatic, heterocyclic, aldehydes, halogenated hydrocarbons, and alkylamine raw materials and the formation of intermediates, and a large amount of acid and alkali needs to be added to the production process to adjust the pH value , causing the pH of the wastewater to be extreme. [0003] Due to its complex production process, berberine production wastewat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/09C12R1/085C12R1/07C12R1/01C02F101/38
CPCC12N1/20C02F3/341C02F3/348C02F2101/38
Inventor 曾萍刘诗月宋永会王鹤立李娟
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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