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Bacillus pumilus for feeds and application of bacillus pumilus

A technology of Bacillus pumilus and bacterial agents, applied in the field of microbiology and feed probiotics, can solve the problems of insufficient stability of live bacteria, less research and application, and inability to exist at the same time, so as to improve feed utilization and improve animal health. Effects of body weight and reduction of feed-to-meat ratio

Active Publication Date: 2019-03-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current problems of micro-ecological products are: 1) The stability of some lactic acid bacteria probiotic products at room temperature is still a difficult problem to solve, which leads to insufficient stability of the viable bacteria in the product during the shelf life; 2) Probiotics after taking probiotics The number of viable bacteria reaching the intestinal tract is affected by gastric acid, intestinal juice, and the stress resistance of the strain itself, and there are great differences between different strains; 3) The germination and proliferation efficiency of passing bacteria such as Bacillus in animals has always been controversial; 4) The physiological activity of probiotics is strain-specific. So far, only a few strains have been proven to have obvious physiological activities, and these probiotic properties cannot exist in the same strain at the same time
However, the research and application of Bacillus pumilus as a probiotic preparation or feed additive is less, especially in the aspects of replacing feed antibiotics and reducing environmental pollution.

Method used

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  • Bacillus pumilus for feeds and application of bacillus pumilus
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  • Bacillus pumilus for feeds and application of bacillus pumilus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and Identification of Bacillus pumilus 13824

[0024] 1. Isolation of strain 13824

[0025] 1. Isolation and cultivation of strains

[0026] Take 1g of the soil sample near the pig breeding base of the West Campus of China Agricultural University in Haidian District, Beijing, and put it into a test tube with 9ml of normal saline, vortex and shake it to make a 1:10 dilution, and then take the dilution to increase by 10 times Dilute, and then select 1ml of each of 3 appropriate gradient dilutions and spread them on the LB medium. Cultivate at 37°C for 48-72 hours, observe and record the colony shape, pick a single colony with good growth, and separate and purify by streaking.

[0027] 2. UV mutagenesis and screening of strains

[0028] Pour the sterilized LB medium into a petri dish, and after solidification, take the bacterial suspension obtained in step 1 and spread it on a plate. Control the number of colonies in each petri dish to about 50. 20c...

Embodiment 2

[0042] Example 2 Stress resistance detection of Bacillus pumilus 13824

[0043] 1. Heat resistance test

[0044] Put the Bacillus pumilus 13824 (CGMCC No.16539) bacterial solution in the water bath for 20 minutes, and treat it at 60°C, 80°C, and 100°C respectively, and repeat each treatment three times. After the treatment, use the pouring method to measure the number of viable bacteria .

[0045] The survival rate of Bacillus pumilus 13824CGMCC No.16539 can reach 99% after being treated at 60°C for 20 minutes, over 90% after being treated at 80°C for 20 minutes, and over 80% after being treated at 100°C for 20 minutes.

[0046] 2. Acid resistance test

[0047] Bacillus pumilus 13824 was inoculated into LB medium with pH values ​​of 2.0, 3.0, 4.0, 5.0, and 6.0, respectively, and the number of viable bacteria was determined by plate pouring method at 1 h, 2 h, 3 h, and 4 h, respectively.

[0048] When the pH is 5.0 or 6.0 in a weakly acidic environment, Bacillus pumilus can ...

Embodiment 3

[0058] The growth curve determination of embodiment 3 bacillus pumilus 13824

[0059] The growth curve represents the dynamic changes in the whole process of bacteria growing and multiplying in a new suitable environment until aging and death. Bacillus pumilus 13824CGMCC No.16539 was inoculated into LB medium at an inoculum amount of 10% (v / v), and cultivated at 37°C for 46 hours. The LB medium without bacterial solution was used as a blank control, and every 2- Measure the OD600 value in 6 hours to calculate the number of viable bacteria. The experiment was repeated three times, the results were averaged, the data were recorded and the growth curve was drawn. Such as Figure 5 As shown, in 0-22 hours, Bacillus pumilus 13824 was in the logarithmic growth phase, and the reproduction rate was higher. The number of B. pumilus 13824 stabilized at 22-46 hours. After 46 hours, the growth rate decline phase was entered.

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Abstract

The invention discloses bacillus pumilus (Bacillus pumilus) 13824 for feeds, wherein the preservation number of the bacillus pumilus 13824 is CGMCC NO.16539. The bacillus pumilus 13824 disclosed by the invention is a gram staining positive bacterium, is resistant to high temperature of 80 DEG C, can grow in the acid environment with pH value being 2.0 or above, is high in cholate resistant capacity, and has the effect of degrading mould teichomycin. After the bacillus pumilus 13824 is prepared into microbial inoculum for feeding animals, the effects are safe and reliable, and the bacillus pumilus 13824 has positive role in the respects of promoting digestion and absorption of nutrient substances, reducing diarrhea rate, improving the transformation efficiency of the feeds, and promoting growth.

Description

technical field [0001] The invention belongs to the field of microbiology and probiotics for feeding, and in particular relates to a Bacillus pumilus for feeding with antibacterial ability, high temperature resistance, acid resistance and bile salt resistance and application thereof. Background technique [0002] At present, food safety issues are attracting more and more attention. Among the many causes of food safety problems, one of the important reasons is the use of antibiotics as growth promoters in feed. For normal bacterial diseases, antibiotics are still the primary treatment; but for promoting animal growth, other green additives are needed to gradually replace the role of antibiotics. Therefore, to screen Bacillus that can effectively inhibit pathogenic microorganisms such as Escherichia coli and Salmonella, and improve animal production performance at the same time, and to develop Bacillus microecological preparations that are stable in feed processing, storage ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23L5/20A61K35/742A61P39/02C12R1/07
CPCA61K2035/115A23K10/18A23L5/28A61P39/02C12N1/20C12N1/205C12R2001/07
Inventor 马曦孙美鸽姬琳堡聂存喜
Owner CHINA AGRI UNIV
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