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Method for microorganism synthesizing glycolipid based on crystal glue

A technology of microbial synthesis and microbial cells, applied in the field of microbial synthesis of glycolipids based on crystal gel, can solve the problems of restricting industrial production and application, high cost of synthesis process, low yield of glycolipids, etc., achieving convenient separation, easy industrial amplification, The effect of improving utilization

Active Publication Date: 2019-03-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the process of microbial synthesis of glycolipids, due to the low density and strong hydrophobicity of the vegetable oil substrate, its dispersion in the fermentation broth mainly depends on mechanical stirring, resulting in uneven dispersion, large mass transfer resistance, and low bioavailability. Substrate inhibition exists under the condition of concentrated substrate, so that the yield of glycolipid is not high, the cost of synthesis process is high, and the energy consumption is large, which greatly limits its industrial production and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 0.02g of hydrophobic matrix crystal gel carrier (pore size 5-200 μm, porosity 85-96%), saturate with 0.05g edible soybean oil and 0.15g edible rapeseed oil, and then put it into 19.8 g YPD liquid medium and 0.2 The fermentation liquid system is formed in the suspended aqueous solution composed of Candida cells; the fermentation liquid system is placed in a constant temperature oscillating bed, and the slow release of the substrate and the microbial fermentation synthesis are carried out under the conditions of a rotation speed of 10 rpm and a temperature of 20 ° C. Fermentation and synthesis The time is 132h, and the pH of the fermentation broth system is 3.5~5.8. Then, the fermented feed liquid was extracted and separated with ethyl acetate and cyclohexane, and the extraction phase was rotary evaporated to remove the ethyl acetate and cyclohexane solvents, and the concentration of glycolipids in the obtained concentrate was calculated to be 1.4 g / L.

Embodiment 2

[0023] Take 0.25g of hydrophobic matrix crystal gel carrier (pore size 5-200 μm, porosity 85-96%), saturated with 1g of edible peanut oil and 2.9g of edible rapeseed oil, and then put into 19.6g of YPD liquid medium and 0.4g of fake The fermentation liquid system is formed in the suspended aqueous solution composed of Trichosanthes cells; the fermentation liquid system is placed in a constant temperature oscillating bed, and the substrate slow release and microbial fermentation synthesis are carried out at a speed of 120 rpm and a temperature of 25 ° C. The fermentation synthesis time is 132 hours , the pH of the fermentation broth system is 3.5~5.8. Then, the fermented feed liquid was extracted and separated by ethyl acetate and cyclohexane, and the extraction phase was rotary evaporated to remove the ethyl acetate and cyclohexane solvents, and the concentration of glycolipids in the obtained concentrate was calculated to be 58 g / L.

Embodiment 3

[0025] Take 0.25g of hydrophobic matrix crystal gel carrier (pore size 5~200 μm, porosity 85~96%), saturated with 3g edible rapeseed oil, and then put into 19.8g YPD liquid medium and 0.2g Candida cells The fermentation broth system was formed in the suspended aqueous solution; the fermentation broth system was placed in a constant temperature oscillating bed, and the substrate slow release and microbial fermentation synthesis were carried out at a speed of 120 rpm and a temperature of 25 ° C. The fermentation and synthesis time was 12 hours. The fermentation broth system pH 4.0~5.8. Then, the fermented feed liquid was extracted and separated with ethyl acetate and cyclohexane, and the extraction phase was rotary evaporated to remove the ethyl acetate and cyclohexane solvents, and the concentration of glycolipids in the obtained concentrate was calculated to be 2 g / L.

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Abstract

The present invention discloses a method for microorganism synthesizing glycolipid based on crystal glue. The method uses super-large pore crystal glue as a hydrophobic substrate release carrier, andcrystal glue controlled release and microorganism metabolism are used to synthesize the glycolipid. In the synthesis process, advantages of large crystal glue carrier porosity, high loading capacity,small mass transfer resistance, high mass transfer rate, easy dispersion and distribution in fermentation liquid, etc. are fully utilized, a hydrophobic substrate is subjected to dispersion and distribution, dispersion of the substrate in the fermentation liquid and contact area of the substrate with microorganism cells are increased, dispersion and mass transfer of the substrate are promoted, andthus utilization of the substrate by microorganisms is improved. At the same time, the crystal glue is utilized to conduct the controlled release of the hydrophobic substrate, substrate inhibition ina microorganism synthesis process is reduced, the microorganism fermentation synthesis process can still be proceed smoothly under a condition of the high concentration substrate, and yield of the glycolipid is improved. The provided method is convenient in downstream separation and easy for industrial amplification, and has a very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for synthesizing glycolipids by microorganisms based on crystal gel. Background technique [0002] Glycolipid microbial surfactants are a very important class of surfactants, which have excellent emulsification, solubilization, dispersion and other surface activities. They have excellent biocompatibility and biosafety, and also have glycoprotein coordination ability and assist Liposomes function for gene delivery and are biodegradable. Therefore, it has a wide range of uses in many industrial fields such as biomedicine, pharmacy, cosmetics, food industry, petroleum, chemical industry and materials. [0003] The chemical synthesis of microbial glycolipids is very difficult, requires the use of highly toxic solvents, causes great environmental pollution, and the synthesis path is very complicated with many steps, so there is no industrial application yet....

Claims

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Application Information

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IPC IPC(8): C12P19/12C12P19/00C12R1/72
CPCC12P19/00C12P19/12
Inventor 贠军贤严琴沈绍传张颂红
Owner ZHEJIANG UNIV OF TECH
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