Method for processing oil crops by using rhodotorula
A technology of oil crops and red yeast, which is applied in the field of fermentation, can solve the problems of long technological process, and achieve the effects of simple operation, cancer prevention and immunity enhancement
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Embodiment 1
[0045] Embodiment 1: Taking soybean puffed powder as raw material
[0046] Specific steps are as follows:
[0047] (1) Connect the Rhodotorula strain stored at 4°C to the YPD seed medium (sterilized at 115°C for 20 minutes), at 30°C, with a rotation speed of 200r min -1 Cultivate for 2 days under certain conditions to obtain the activation solution;
[0048] (2) Take 80mL of YPD seed medium and place it in a 250mL Erlenmeyer flask (sterilized at 115°C for 20min), take 200μL of the activation solution obtained in (1) and inoculate it into the YPD seed medium, at a temperature of 30°C and a rotation speed of 200r·min -1 Cultivate for 2 days under the condition of 9 CFU / mL;
[0049] (3) Take 300g of fermentation medium and place it in a 2500mL Erlenmeyer flask (sterilized at 121°C for 30min), inoculate the seed liquid into the fermentation medium according to the fermentation parameters in Table 1 for solid-state fermentation to obtain a fermented product.
[0050] The obtain...
Embodiment 2
[0063] Embodiment 2: Taking peanut powder as raw material
[0064] Specific steps are as follows:
[0065] (1) with embodiment 1;
[0066] (2) with embodiment 1;
[0067] (3) Put the peanuts into a hot air circulation oven, dry them at 50°C, and dry them until the water content is 5-10%, then peel them off, and grind the peeled peanuts into fine powder to obtain peanuts pink;
[0068] (4) Replace soybean puffed powder with peanut powder, take 300g of fermentation medium and place it in a 2500mL Erlenmeyer flask (sterilized at 121°C for 30min), inoculate the seed liquid into the fermentation medium according to the fermentation parameters in Table 1 for solid-state fermentation, and obtain Fermentation.
[0069] The obtained fermented product is subjected to oil extraction according to the method of functional oil and oil crop meal analysis to obtain functional oil and fermented soybean meal; the obtained functional oil and fermented soybean meal are analyzed according to t...
Embodiment 3
[0070] Embodiment 3: take rapeseed powder as raw material
[0071] Specific steps are as follows:
[0072] (1) with embodiment 1;
[0073] (2) with embodiment 1;
[0074] (3) Put the rapeseed into a hot air circulation oven, dry it at 50°C, and dry it until the water content is 5-10%, then peel it off, and grind the shelled rapeseed into fine powder, get rapeseed meal;
[0075] (4) Replace soybean puffed powder with rapeseed powder, take 300g of fermentation medium and place it in a 2500mL Erlenmeyer flask (sterilized at 121°C for 30min), inoculate the seed liquid into the fermentation medium according to the fermentation parameters in Table 1 for solid-state fermentation, Get a ferment. The obtained fermented product is subjected to oil extraction according to the method of functional oil and oil crop meal analysis to obtain functional oil and fermented soybean meal; the obtained functional oil and fermented soybean meal are analyzed according to the method of functional ...
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