Application of carbon quantum dots-graphite-like carbon nitride photocatalytic material in the preparation of drugs for sterilization and skin scar healing
A graphitic carbon nitride and carbon nitride light technology, which is applied in the field of photocatalytic materials, can solve the problems of low energy consumption, low efficiency, production of carcinogenic by-products, etc., achieves strong photocatalytic activity, obvious bactericidal effect, and promotes quick healing effect
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Embodiment 1
[0046] Example 1CQD s / g -C 3 N 4 preparation of
[0047] 1. Graphite Carbon Nitride (Graphite Carbon Nitride, g-C 3 N 4 )Synthesis
[0048] Put urea (30g) in a ceramic crucible with a lid, and calcined at 550°C for 2 hours at a heating rate of 4°C / min to obtain a light yellow powder, namely g-C 3 N 4 .
[0049] 2. Synthesis of Carbon Quantum Dots (CQDs)
[0050] Dissolve 3g of citric acid and 1mL of ethylenediamine in 30mL of deionized water to obtain a mixed solution I; then transfer the mixed solution I to a 50mL hydrothermal synthesis reactor and heat at 200°C for 5 hours; after the reaction, the reaction The device was naturally cooled to room temperature, and dried at 80° C. for 24 hours to obtain a dark brown powder, which was carbon quantum dots.
[0051] 3. Carbon quantum dots-graphite-like carbon nitride (CQDs / g-C 3 N 4 )Synthesis
[0052] Mix 6mg CQDs with 30mL water to obtain mixed solution II, then add 200mg g-C 3 N 4 Dispersed in mixed solution II; ...
Embodiment 2
[0062] Embodiment 2 carbon quantum dots-like graphitic phase carbon nitride (CQDs / g-C 3 N 4 ) in killing Staphylococcus aureus
[0063] 1. Combine CQDs / g-C 3 N 4 Interact with SA to detect the survival of SA. Samples were taken every 20 minutes, and the number of SA survivors was calculated by standard plate count method.
[0064] S. aureus was cultured in tryptic soy broth (TSB) medium and then resuspended in phosphate buffered saline (PBS, 0.01 M, pH 7.4) to construct the final S. aureus suspension (10 7 CFU mL -1 ). For each sterilization experiment, 50 mL of Staphylococcus aureus suspension and 50 mg of photocatalyst powder were mixed in a beaker. The bacteria and photocatalyst were uniformly mixed on a magnetic stirrer at room temperature while irradiating with a 300W xenon lamp equipped with a UV cut filter (λ-2 . As the reaction proceeds, the mixture is carefully pipetted at predetermined intervals and the bacterial concentration is determined by plate counting....
Embodiment 3
[0071] Embodiment 3 carbon quantum dots-like graphitic phase carbon nitride (CQDs / g-C 3 N 4 ) in the repair of Staphylococcus aureus infected skin
[0072] 1. Divide 6-8 week old C57BL / 6 female mice into untreated group, CQDs / g-C 3 N 4 group and g-C 3 N 4 Group.
[0073] First, a 1.5% amobarbital sodium solution was prepared, and mice were anesthetized by intraperitoneal injection in an amount of 120 μl / 20 g to construct a SA skin infection model.
[0074] Then, 80 mm of skin was made on the back skin of mice in each group. 2 area of sterile wound, place 1 x 10 7 CFU of Staphylococcus aureus smeared on the window, followed by 100mg of CQDs / g-C on the back 3 N 4 or g-C 3 N 4 , under light conditions for 2 hours.
[0075] The area and bacterial load of skin scars in each group were observed.
[0076] It was found that: with g-C 3 N 4 Group comparison, CQDs / g-C 3 N 4 Skin scars healed faster in mice in the group (see Figure 6 ).
[0077] 2. Combine CQDs / g-C ...
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