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Water phase extraction method of erythromycin fermentation broth

A water-phase extraction and fermentation liquid technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of high cost, increased cost, and high cost of the extraction process, and achieve simple and easy resin operation, The effect of improving the quality of finished products and reducing extraction costs

Active Publication Date: 2019-04-02
UNITED LAB INNER MONGOLIA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Converted to about 25-30 yuan per kilogram of product cost, the cost is very high; and zinc sulfate is a heavy metal, and the filtered bacterial residue is not easy to handle; 2) The extraction process is expensive, and the erythromycin extraction process needs to consume a large amount of solvent, solvent Requires extensive acid-base cleaning for reuse
In the process of washing the solvent, the waste water after extraction and separation needs to be recycled, so the cost is relatively high; 3) The investment and operation cost of the centrifuge is high, and the extraction requires a large number of centrifuges for centrifugation, which requires large investment and high energy consumption. Due to the operation in the solvent environment, there is a certain risk
4) waste water treatment containing organic solvent, this extraction method produces a large amount of waste water containing a small amount of organic solvent, the treatment of this waste water needs to invest a certain amount of money, and then improves the cost
This method only uses ceramic membranes with smaller pore size when processing the fermentation broth, and the processing capacity of the ceramic membrane to the fermentation broth is low, and the filtrate passes through the strong basic anion exchange resin, and the resin has the disadvantages of low processing capacity of the filtrate.

Method used

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  • Water phase extraction method of erythromycin fermentation broth
  • Water phase extraction method of erythromycin fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 200L of erythromycin fermentation broth, with a titer of 8000u / ml, was filtered through a 50nm ceramic membrane at a temperature of 8°C, and the resulting filtrate was ultrafiltered through a coiled membrane with a molecular weight cut-off of 30,000. The obtained ultrafiltrate is purified by LX-98 resin column to obtain a column liquid, and the column liquid is nanofiltered through a nanofiltration membrane with a molecular weight cut-off of 100 until the titer of the concentrated solution is 15000u / ml. In the concentrated solution, add sodium thiocyanate with a molar ratio of 1.0 to erythromycin, finish adding in 1 hour, continue to react for 1 hour after adding, use 15% glacial acetic acid for 30 minutes to adjust the pH to 4.6, and precipitate erythromycin thiocyanate, Continue to stir for 10 minutes, centrifugally filter, and wash the filter cake with hot water at 55°C, and the dosage is 1B of the volume of the mother liquor. Filtrate, and when the wet powder is dri...

Embodiment 2

[0057] 500L of erythromycin fermentation broth, with a titer of 8000μ / ml, was filtered through a 50nm ceramic membrane at a temperature of 10°C, and the resulting filtrate was ultrafiltered through a coiled membrane with a molecular weight cut-off of 50,000. The obtained ultrafiltrate is purified by a LX-98 resin column to obtain a column solution, and the column solution is subjected to nanofiltration through a nanofiltration membrane with a molecular weight cut-off of 200 until the titer of the concentrated solution is 25000u / ml. In the concentrated solution, add sodium thiocyanate with a molar ratio of 1.5 to erythromycin, finish adding in 1.5h, continue to react for 0.5h after adding, adjust pH to 4.7 with 20% glacial acetic acid for 20min, and precipitate erythromycin thiocyanate , continue to stir for 20 minutes, centrifuge and filter, and wash the filter cake with hot water at 55° C., the dosage is 1.5B of the volume of the mother liquor. Filter and dry the wet powder a...

Embodiment 3

[0060] 1000L of erythromycin fermentation broth with a titer of 8000u / ml was filtered through a 50nm ceramic membrane, and the resulting filtrate was ultrafiltered through a coiled membrane with a molecular weight cut-off of 35,000. The obtained ultrafiltrate is purified by one or more LX-98 resin columns to obtain the column solution, which is then subjected to nanofiltration through a nanofiltration membrane with a molecular weight cut-off of 500 until the titer of the concentrated solution is 20000 μ / ml. In the concentrated solution, add sodium thiocyanate with a molar ratio of 1.2 to erythromycin, add it in 2 hours, continue the reaction for 0.5 minutes, use 25% glacial acetic acid aqueous solution to adjust the pH to 4.8 within 30 minutes, and precipitate erythromycin thiocyanate. Continue to stir for 10 minutes, centrifugally filter, and wash the filter cake with hot water at 55°C, and the dosage is 2B of the volume of the mother liquor. Filtrate, and dry the wet powder ...

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Abstract

The invention belongs to the field of medicine preparation, and more specifically relates to a water phase extraction method of an erythromycin fermentation broth. The water phase extraction method comprises following steps: the erythromycin fermentation broth is subjected to ceramic membrane filtration without adding of a flocculating agent so as to obtain a filtrate, the filtrate is subjected toultrafilter membrane ultrafiltration, and an obtained ultrafiltrate is subjected to resin column purification; an obtained column purified liquid is subjected to nanofiltration membrane nanofiltration concentration so as to obtain a concentrate, sodium sulfocyanate and glacial acetic acid are added into the concentrate for precipitation of erythromycin thiocyanate, filtering is carried out so asto obtain a erythromycin thiocyanate wet powder, and drying is carried out so as to obtain a erythromycin thiocyanate dry powder. According to the water phase extraction method, membrane technology and resin purifying technology are adopted to replace conventional frame and plate filtration and solvent extraction purification concentration methods, resin processing amount is large, and erythromycin production cost is reduced obviously. The water phase extraction method is large in subsequent optimization potential, and is capable of improving finished product quality effectively, increasing yield, and improving product competitiveness.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to an aqueous phase extraction method of erythromycin fermentation liquid. Background technique [0002] Erythromycin is an important macrolide antibiotic. It is industrially obtained by microbial fermentation. Its downstream separation and purification process has always been the key to the production of erythromycin. The traditional extraction process of erythromycin is: After chemicalization and flocculation treatment, it is filtered with a plate frame, and the filtrate is extracted with a solvent and centrifuged to separate to obtain a solvent phase. Add sodium thiocyanate and glacial acetic acid to the solvent phase to crystallize erythromycin thiocyanate, wash and dry the crystals to obtain erythromycin thiocyanate. This process has been used for a long time and the process is stable. Its main disadvantages are: 1) a large amount of flocculant is required, and before filtering the er...

Claims

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Application Information

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IPC IPC(8): C07H17/08C07H1/06
CPCC07H1/06C07H17/08
Inventor 何同鹏吴艳菲王克玉
Owner UNITED LAB INNER MONGOLIA CO LTD
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