Detection method of nosiheptide residue in animal food
A technology of animal food and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low nosiheptide concentration, no significant progress in high performance liquid chromatography analysis, and high detection limit, and achieve simple operation, The effect of complete hydrolysis reaction and mild action conditions
Active Publication Date: 2019-04-05
SOUTH CHINA AGRI UNIV
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Problems solved by technology
However, due to the low concentration and high detection limit of nosiheptide in animal food or plasma, high performance liquid chromatography analysis has not made significant progress in the field of drug residue detection and pharmacokinetic research
So far there is no LC-MS / MS (liquid chromatography-mass spectrometry) method for the detection of nosiheptide residues reported
Method used
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Embodiment 1
[0069] Preparation of Nosiheptide Hydrolyzate 4-Hydroxymethyl-3-Methylindole-2-carboxylic Acid
[0070] Weigh 5g of nosiheptide raw material into a 250mL single-necked flat bottom flask, add 200mL of water, under magnetic stirring, after mixing evenly, slowly add 50mL of 4mol / L sodium hydroxide solution, and stir at room temperature for 2h. Transfer the reaction solution to a 50mL centrifuge tube, centrifuge and stir at 4°C and 9000 rpm for 10min, then transfer the supernatant to a 250mL tetrafluoroethylene stoppered separatory funnel, and wash with 50mL n-hexane and ethyl acetate respectively Esters were extracted and washed 3 times; the layers were left to stand, the lower layer was transferred to a 250mL beaker, the pH value was adjusted to 3-4 with 6mol / L hydrochloric acid, extracted 3 times with 50mL ethyl acetate, combined with ethyl acetate, and spun to nearly After drying, pour 2 mL of ethanol to redissolve, centrifuge and stir at 4°C and 9000 rpm for 5 minutes, then r...
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Abstract
The invention provides a detection method of nosiheptide residue in an animal food, and belongs to the field of veterinary drug residue detection. The detection method of the nosiheptide residue in the animal food comprises the following steps that after muscular tissue of the animal food is sequentially subjected to aqueous alkali hydrolyzing, grease removal and pH value adjusting, solid-phase extraction elution is carried out, and an eluent is obtained; the eluent is sequentially subjected to blowing drying, dissolution and filtration, and a filtrate is obtained; and the filtrate is subjected to liquid chromatogram-tandem mass spectrometry analysis, the content of 4-hydroxymethyl-3-methylindole-2-formic acid is detected, and the content of the nosiheptide residue in the animal food is obtained by calculating according to the content of the 4-hydroxymethyl-3-methylindole-2-formic acid corresponding to the nosiheptide standard. The detection method of the nosiheptide residue in the animal food has the characteristics of high selectivity and high sensitivity, and can efficiently detect the content of the nosiheptide residue in animal muscular tissue.
Description
technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to a detection method for nosiheptide residues in animal food. Background technique [0002] Nosiheptide is a sulfur-containing polypeptide antibiotic produced by Streptomyces, which can effectively inhibit the growth of Gram-positive bacteria. It is widely used in animal husbandry to prevent and treat bacterial infections such as respiratory diseases and enteritis. At the same time, nosiheptide can effectively promote animal growth, and is often added to feed as a growth-promoting agent to improve the growth rate and feed conversion rate of animals such as pigs, poultry, and fish. However, due to the lack of awareness of the potential hazards of drug residues among farmers, the phenomenon of abuse and irrational use of drugs in actual production may lead to nosiheptide residues in animal foods, entering the human body through the food chain and endangering human...
Claims
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IPC IPC(8): G01N30/88G01N30/06
Inventor 贺利民谢景梦曾振灵汤有志苏贻娟
Owner SOUTH CHINA AGRI UNIV