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Carotenoid-protein particle, and preparation method and application thereof

A carotene and protein technology, applied in the field of carotenoid-protein particles and its preparation, can solve the problems of difficult carotenoids, large application limitations, and poor processing suitability, and achieve no chemical residues, easy storage and transportation, and low production costs. low effect

Active Publication Date: 2019-04-09
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of poor processing suitability and large application limitations of carotenoids due to poor water solubility, poor stability and low absorption rate, the present invention provides a carotenoid with good water solubility, strong stability, Carotenoid-protein microparticles with high absorption rate and its preparation method and application, the preparation method has low cost, the prepared carotenoid-protein microparticles have high safety, and can be widely used in food systems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Weigh 0.5g of bovine serum albumin, add 99.5mL of deionized water, fully stir to dissolve, adjust the pH value to 12 with sodium hydroxide, so that the protein hydrophobic inner cavity is opened, and a concentration of 0.5wt% alkali is obtained. aqueous protein solution;

[0034] (2) Weigh 3.0g of β-carotene powder, fully dissolve it with a small amount of 0.1wt% sodium hydroxide ethanol solution (the mass ratio of β-carotene powder to sodium hydroxide ethanol solution is 1:1), then add 9.0 gram of oleic acid, stirred evenly, heated to fully dissolve the carotenoids, to obtain a β-carotene-fatty acid dispersion with a β-carotene concentration of 20wt%;

[0035] (3) Slowly add 10.0 g of β-carotene dispersion into all the aqueous alkaline protein solutions obtained in step (1), stir evenly, and ultrasonically treat for 2 hours to fully combine β-carotene with the hydrophobic inner cavity of the protein;

[0036] (4) adding zinc sulfate to the solution obtained in ste...

Embodiment 2

[0040] (1) Weigh 1.5g of whey protein, add 98.5mL of deionized water, fully stir to dissolve, adjust the pH value to 11 with sodium hydroxide, so that the protein hydrophobic inner cavity is opened, and the concentration of 1.5wt% alkaline protein aqueous solution;

[0041] (2) Weigh 4.5g of lutein powder, fully dissolve with a small amount of 0.5wt% sodium hydroxide propylene glycol solution (the mass ratio of lutein powder and sodium hydroxide propylene glycol solution is 1:0.8), then add 10.5g of Linoleic acid, stirred evenly to fully dissolve the lutein to obtain a lutein dispersion with a lutein concentration of 24.2 wt%.

[0042] (3) Slowly add 15.0 g of lutein dispersion into all the alkaline protein aqueous solution obtained in step (1), stir evenly, and ultrasonically treat for 1 hour, so that the lutein is fully combined with the hydrophobic inner cavity of the protein;

[0043] (4) adding zinc sulfate to the solution obtained in step (3) to a final concentration of...

Embodiment 3

[0047] (1) Weigh 4.0g of casein, add 96mL of deionized water, fully stir to dissolve, adjust the pH value to 10 with sodium hydroxide, so that the protein hydrophobic inner cavity is opened, and obtain an alkaline protein aqueous solution with a concentration of 4.0wt%. ;

[0048] (2) Take by weighing 1.2g of zeaxanthin powder, fully dissolve with a small amount of 0.8wt% sodium hydroxide polyethylene glycol solution (the mass dosage ratio of zeaxanthin powder and sodium hydroxide polyethylene glycol solution is 1:0.5 ), then add the linolenic acid of 1.2g, stir well, make the zeaxanthin fully dissolve, obtain the zeaxanthin dispersion liquid that the zeaxanthin concentration is 40wt%;

[0049] (3) Slowly add 4.0 g of zeaxanthin dispersion into all the aqueous alkaline protein solutions obtained in step (1), stir evenly, and ultrasonically treat for 1.5 hours, so that zeaxanthin is fully combined with the hydrophobic inner cavity of the protein;

[0050] (4) Add zinc sulfate ...

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Abstract

The invention belongs to the field of food and discloses a carotenoid-protein particle, and a preparation method and an application of the carotenoid-protein particle. The preparation method of the carotenoid-protein particle comprises the steps of dissolving a carrier protein with water, regulating a pH (potential of hydrogen) to 10-12 to form an alkaline protein aqueous solution, dissolving carotenoid powder with a cosolvent containing sodium hydroxide, adding fatty acid, performing uniform stirring to form carotenoid-fatty acid dispersing liquid, mixing the alkaline protein aqueous solutionand the carotenoid-fatty acid dispersing liquid, performing ultrasonic treatment, mixing with zinc sulfate for reaction, regulating a pH of a reaction product obtained to 6-8, and performing direct spray-drying on an obtained carotenoid-protein compound aqueous solution or freeze drying after vacuum concentration. The carotenoid-protein particle prepared by the method has the advantages of a microcapsule and nano emulsion and the characteristics of strong stability, good absorption effect, simple production technology and no chemical residue.

Description

technical field [0001] The invention relates to the field of food, in particular to a carotenoid-protein particle and its preparation method and application. Background technique [0002] Carotenoids have disadvantages such as poor water solubility and difficulty in dispersing in the body, which make it difficult to disperse evenly in food hydrophilic systems and cause multiple absorption barriers. Therefore, constructing a suitable intestinal absorption delivery carrier is the technical key to solve the solubility, stability and absorbability of carotenoids. [0003] Conventional microencapsulation technology usually emphasizes the improvement of carotenoid loading, solubility and stability, but ignores the absorption effect of the human body, which violates the original intention of nutritional fortification. my country only started to enter the carotenoid formulation industry in the past 15 years, represented by NHU and Xinchang Pharmaceutical. For example, β-carotene w...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/15A23P10/20
CPCA23L33/105A23L33/15A23P10/20A23V2002/00A23V2250/54A23V2250/211
Inventor 刘翼翔傅秋叶刘光明孙乐常
Owner JIMEI UNIV
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