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High-efficiency power generation and denitrification method of microbial fuel cell with a high-salt and nitrogen-containing wastewater matrix

A fuel cell and microbial technology, applied in chemical instruments and methods, biological water/sewage treatment, electrochemical-biological combination treatment, etc., can solve problems such as increased operating costs, no application value, and poor stability of microbial activity in salt-tolerant electrodes. To achieve the effect of improving denitrification ability, strengthening denitrification ability and improving efficiency

Active Publication Date: 2019-04-12
DALIAN MARITIME UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the domesticated salt-tolerant electrode microorganisms have poor activity stability; pure strains (or single types) of halophilic bacteria have no application value as electrode microorganisms (the wastewater matrix is ​​generally a mixed flora); the added stress resistance additives are expensive and greatly increase running cost
[0007] Although the high-salt wastewater matrix will reduce the internal resistance of MFC, high salt will also inhibit microbial activity and reduce the efficiency of electricity production and nitrogen removal
The current methods to improve the salt tolerance of MFC microorganisms are not of practical value

Method used

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  • High-efficiency power generation and denitrification method of microbial fuel cell with a high-salt and nitrogen-containing wastewater matrix
  • High-efficiency power generation and denitrification method of microbial fuel cell with a high-salt and nitrogen-containing wastewater matrix
  • High-efficiency power generation and denitrification method of microbial fuel cell with a high-salt and nitrogen-containing wastewater matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Inhibition of high-salt substrates on microbial fuel cell electricity production

[0042] Take 5 bipolar single-chamber MFCs that operate stably in 0g / L NaCl MFC matrix, set the NaCl concentration of the MFC matrix to 0, 15, 30, 45, 60g / L, respectively, and culture them in a 30°C incubator. Use the DT9205L multimeter to measure the voltage every 1h. Three parallel experimental samples were set up for each same experiment. The same below.

[0043] Inhibition of high-salt substrates on power generation by microbial fuel cells figure 1 . figure 1 It was shown that as the NaCl concentration in the MFC matrix increased, the voltage of the MFC also gradually decreased. When the NaCl concentration increased from 0g / L to 15, 30, 45, and 60g / L, the average voltage within 24 hours decreased by 10.1%, 35.6%, 43.1%, and 54.5%, respectively. The results showed that the high-salt substrate had a significant inhibitory effect on the electricity production of the microb...

Embodiment 2

[0044] Example 2.H.alkaliphila DSM 16354 synthesizes and secretes ectoine

[0045] Ectoine concentration determination method. Determination of extracellular ectoine concentration: ectoine-induced synthetic culture medium was centrifuged at 14000×g, and the supernatant was diluted 10 times with distilled water for HPLC determination (ectoine secreted by cells). Determination of intracellular ectoine concentration: Centrifuge according to the above method, collect the precipitated cells, wash with NaCl-Kpi buffer solution (100mM, pH7.2, the concentration of NaCl is the same as that of the medium), and add the same volume of culture medium to the centrifuged precipitate Extracted with 80% ethanol (v / v), resuspended, and left at room temperature overnight. The suspension was centrifuged again, and the supernatant was taken for HPLC analysis (for intracellular ectoine). The total concentration of ectoine is the sum of extracellular and intracellular ectoine concentrations. The ...

Embodiment 3

[0047] Example 3. H. alkaliphila DSM 16354 SND denitrification

[0048] Total inorganic nitrogen (TN) including ammoniacal nitrogen Nitrite nitrogen Nitrate nitrogen The denitrification rate is defined as the percentage of the reduced TN in the denitrification system to the initial TN. Nitrogen removal rate = (TN 0 -CN-TN t ) / (TN 0 -CN) × 100%. where NT 0 TN is the initial denitrification, CN is the total nitrogen of the cell, TN t is the TN at a certain moment in the denitrification process. Determination by Nessler's reagent method concentration. Determination of diazo-azo method Determination of concentration zinc cadmium reduction method concentration. Cellular total nitrogen (CN) concentration was determined by Kjeldahl method to determine CN. The CDW with different masses was used to measure CN, and the relational expression between CDW and CN was fitted. In the experiment, CN is calculated by measuring CDW.

[0049] 5mL LB (60g / L NaCl) medium, 30°C...

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Abstract

The invention discloses a high-efficiency power generation and denitrification method of a microbial fuel cell with the high-salt and nitrogen-containing wastewater matrix, and belongs to the technical field of bioenergy. An immobilized cell phase is arranged in a traditional bipolar single-chamber microbial fuel cell (MFC) to construct a three-phase single-chamber MFC. The immobilized cell is ectoine secreting type halomonas meridiana with simultaneous nitrification and denitrification functions. The salt tolerance assistant function and simultaneous nitrification and denitrification functionof halomonas meridiana are integrated into an MFC to improve the salt tolerance of microorganisms at an MFC electrode and enhance the efficiency of SND denitrification at high salinity. The method solves the problems that a high salt wastewater matrix can reduce the internal resistance of the MFC, but meanwhile the activity of microorganisms can be inhibited and the efficiency of power generationand denitrification is reduced. The method is of great significance to the utilization of the MFC in high salt wastewater.

Description

technical field [0001] The invention belongs to the technical field of biological energy, and in particular relates to a method for efficient power generation and denitrification by microbial fuel cells in high-salt nitrogen-containing wastewater substrates. Background technique [0002] Microbial fuel cell (MFC) is a device and technology that uses microorganisms as catalysts to oxidize organic or inorganic substances to generate current [Bruce E. Logan, Bert Hamelers, Rene Rozendal, et al. Microbial fuel cells: methodology and technology. Environmental Science & Technology, 2006, 40(17):5181-5192]. MFC power generation and wastewater purification coupling technology is a new type of energy-saving and productive wastewater treatment technology that integrates wastewater recycling, sludge reduction and water quality harmlessness. It has been used in municipal or domestic wastewater, agricultural animal manure wastewater, and food processing wastewater. Related studies have ...

Claims

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Application Information

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IPC IPC(8): C02F3/00C02F3/30C02F3/34C02F101/16C02F101/38
CPCC02F3/005C02F3/302C02F3/34C02F2101/16C02F2101/38Y02P70/50
Inventor 张苓花曾繁锦刘伟凤朱益民王特
Owner DALIAN MARITIME UNIVERSITY
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