Process for extracting astaxanthin from haematococcus pluvialis by using supercritical CO2

Abstract

The invention discloses a process for extracting astaxanthin from haematococcus pluvialis by using supercritical CO2, which comprises the following steps of: (1) stirring haematococcus pluvialis powder, an adhesive and auxiliary materials in a stirrer, placing the stirred mixture in a screw extruder for granulation, then sending the wet particles into a low-temperature dryer for vacuum low-temperature drying to obtain haematococcus pluvialis particles; then putting the haematococcus pluvialis particles prepared in step 1 into an extraction kettle, and adding a carrying agent into the extraction kettle for extraction to obtain an astaxanthin extract; and finally, placing the astaxanthin extract prepared in step (2) into a rotary distillation kettle, and performing rotary evaporation to remove residual carrying agent to obtain high-purity astaxanthin. According to the method, the procedures of granulation, re-extraction, separation and purification are adopted, and specific separation conditions are combined, so that the extraction time of the astaxanthin is shortened, the extraction rate of the astaxanthin is greatly improved, and the purity of the obtained astaxanthin product is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to a supercritical CO 2 Process for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Haematococcus pluvialis is a widely distributed unicellular green algae, which can form thickened spores and accumulate a variety of carotenoids with physiological functions under stress conditions: astaxanthin, α-carotene, β-carotene lutein, cryptoxanthin, zeaxanthin, canthaxanthin, etc., 80% of which are astaxanthin and its esters. Astaxanthin is a natural carotenoid, which is fat-soluble and pink in color. It contains unsaturated compounds in terpenes. It is a strong antioxidant that can quench superoxide radicals and singlet oxygen ions. Clinical and animal experiments have proved that astaxanthin has various biological functions such as increasing immune function, inhibiting tumor occurrence, preventing cardiovascular diseases, maintaining eyes ...

AI Technical Summary

Problems solved by technology

At present, the use of supercritical extraction of Haematococcus pluvialis is mainly to first break the wall of Haematococcus pluvialis into fine powder, and then perform supercritical CO 2 Extraction, when using this extraction method to extract astaxanthin, there are the following deficiencies: First, during the extraction process of Haematococcus pluvialis powder, the algal powder is easy to accumulate and agglomerate, so that the contact area between the algal powder and the extractant is small , the solvent is difficult to penetrate into the algal cells, so that the astaxanthin in the algae powder cannot be completely dissolved, the extraction rate of astaxanthin is low, and the extraction rate of astaxanthin can only reach about 50%; the third is the astaxanthin after supercritical The purity of astaxanthin is low, and the extracted astaxanthin contains a large amount of solvent and ester mixture, and its purity can only reach about 20%.