Bacillus HY-1 and application thereof in degradation of organic pollutants
A technology of organic pollutants and Bacillus, applied in the field of high-efficiency new n-hexane degrading bacteria, can solve problems such as undiscovered
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Embodiment 1
[0032] Example 1: Isolation, purification and identification of Bacillus HY-1
[0033] (1) Isolation and purification of Bacillus HY-1
[0034] The activated sludge from the sewage treatment tank of a pharmaceutical factory in Zhejiang was collected on site, and the lower sludge after standing still was mixed with inorganic salt medium at a ratio of 1:2 (v / v), and 3L of the mixed solution was added to a 5L sludge acclimation tank ( For the structure, please refer to Jin Xiaojun. Isolation and identification of new dioxane-degrading strains, degradation characteristics and preparation of bacterial agents [D]. Zhejiang University of Technology, 2012.), add 500mg / L substrate n-hexane as the only carbon source and energy every day, room temperature After nearly 20 days, 5mL of sludge was taken from the acclimatization tank and added to a shake flask containing 50mL of inorganic salts. It was found that the acclimatized sludge could stably degrade 130mg / L n-hexane per day in the sh...
Embodiment 2
[0042] Embodiment 2: the seed liquid preparation of bacillus HY-1
[0043] 1) Slant culture: inoculate Bacillus HY-1 on the LB solid medium of the slant, and culture at 30° C. for 18-26 hours to obtain slant bacteria.
[0044] 2) Seed culture: use an inoculation loop to pick the slant bacteria obtained in step 1) and inoculate them into LB liquid medium, culture at 30°C for 24-36 hours, and obtain OD 600 =0.1~0.2 bacteria solution.
Embodiment 3
[0045] Embodiment 3: the performance of bacillus HY-1 degradation n-hexane
[0046] With normal hexane as the only carbon source of bacillus HY-1, get the OD prepared by the method in Example 2 600 =0.1 bacterium solution, inoculate to fresh 50mL inorganic salt medium containing n-hexane with a final concentration of 155mg / L, so that the initial bacterium concentration is expressed as OD 600 Calculated as 0.01, pH=7. Put it into a shaker with a temperature of 30°C and a rotation speed of 160r / min for cultivation, take samples every 5 hours to measure the degradation rate of n-hexane, and use a 5mL syringe to draw out a part of the bacterial liquid to measure the OD value of the bacterial cells. The results are shown in image 3 . During the experiment, two parallel samples and a blank control group not inoculated with strains were designed. With the prolongation of time, the bacterial cell concentration gradually increased, and at 24 hours, the bacterial cell concentration ...
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