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Preparation method of solid lactic acid bacteria high-activity bacterial agent for raising

A lactic acid bacteria, high-activity technology, applied in the field of bioengineering, can solve the problems of single nutrient and probiotics, low fermentation bacteria, and limited fermentation scale.

Inactive Publication Date: 2019-04-19
SHANDONG CHENZHANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process described in this invention is simple and easy, and the product is easy to preserve, but the activity of the fermentation bacteria is not high, and the product has a single nutritional prebiotic
Patent CN 1400304 A discloses a liquid-solid combined fermentation process of probiotics. The solid fermentation process of this invention is carried out in anaerobic plastic bags, and the solid matrix components are simple and the fermentation scale is limited.

Method used

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  • Preparation method of solid lactic acid bacteria high-activity bacterial agent for raising
  • Preparation method of solid lactic acid bacteria high-activity bacterial agent for raising

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] First, prepare 12% (W / V) skimmed milk powder, add 3% trehalose and 0.3 mM glutathione, and sterilize at 110°C for 10 minutes. After cooling to room temperature, insert Bifidobacterium bifidum in a ratio of 3%, and ferment to produce live lactic acid bacteria agent. Ferment at a constant temperature of 35°C for 6 hours until the skim milk powder coagulates, and the fermentation ends when the pH is 4.5. After stirring at 150 rpm and cooling to room temperature, the active lactic acid bacteria agent was obtained, and the effective number of viable bacteria was 2.0×10 9 cfu / mL.

[0029] Then, the above-mentioned live lactic acid bacteria agent is formulated into a basic formula solid preparation. Mix and stir the live lactic acid bacteria agent with each component, and add water while stirring. In terms of parts by weight, the components are: 5 parts of xylooligosaccharide, 4 parts of sulfomethylcellulose sodium, 25 parts of sodium alginate, 50 parts of cornstarch and 40...

Embodiment 2

[0034] First, prepare 15% (W / V) skimmed milk powder, add 2% pullulan, 2% resistant starch and 0.3% mM iso-VC acid, and sterilize at 110°C for 12 min. After being cooled to room temperature, inoculate according to the ratio of 3% Lactobacillus lactis and 2% Bacillus bulgaricus, and ferment to produce live lactic acid bacteria agent. Ferment at a constant temperature of 39°C for 5 hours until the skim milk powder coagulates, and the fermentation ends when the pH is 4.0. After stirring at 200 rpm and cooling to room temperature, the active lactic acid bacteria agent was obtained, and the effective number of viable bacteria was 3.6×10 9 cfu / mL.

[0035] Then, the above-mentioned live lactic acid bacteria agent is formulated into a basic formula solid preparation. Mix and stir the live lactic acid bacteria agent with each component, and add water while stirring. In parts by weight, each component is: 10 parts of fructooligosaccharide, 8 parts of microcrystalline cellulose, 10 pa...

Embodiment 3

[0040] First, prepare 15% (W / V) skimmed milk powder, add 3.5% (W / V) trehalose and 0.5% montmorillonite, and sterilize at 108°C for 12 min. After cooling to room temperature, insert Lactobacillus acidophilus at a ratio of 3%, and ferment to produce live lactic acid bacteria agent. Ferment at a constant temperature of 37°C for 6 hours until the skim milk powder coagulates, and the fermentation ends when the pH is 4.3. After stirring at 180 rpm and cooling to room temperature, the active lactic acid bacteria agent was obtained, and the effective number of viable bacteria was 2.5×10 9 cfu / mL.

[0041] Then, the above-mentioned live lactic acid bacteria agent is formulated into a basic formula solid preparation. Mix and stir the live lactic acid bacteria agent with each component, and add water while stirring. In parts by weight, each component is: 5 parts of xylooligosaccharide, 5 parts of sodium sulfamethycellulose, 35 parts of polyacrylic resin II, 50 parts of wheat starch an...

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Abstract

The invention relates to a preparation method of a solid lactic acid bacteria high-activity bacterial agent for raising. The preparation processes of the lactic acid bacteria active bacterial agent comprise fermentation of a probiotic lactic acid bacteria living bacteria agent, a basic formula of a lactic acid bacteria solid bacterial agent, preparation of the formula and a powder granulation process. The preparation method of the solid lactic acid bacteria high-activity bacterial agent for raising has the advantages that through the addition of various bioactive components in the fermentationprocess of the lactic acid bacteria living bacteria agent and optimization of the basic formula and protection formula, the probiotic function of the bacterial agent is improved while the high survival rate of lactic acid bacteria is ensured; the acid resistance and bile salt tolerance of the lactic acid bacteria in the prepared lactic acid bacteria active bacterial agent are improved, the survival rate of the lactic acid bacteria in the simulated animal gastrointestinal fluid is high, and the lactic acid bacteria active bacterial agent has a good probiotic healthcare function.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a preparation method of a high-activity bacterium agent of solid lactic acid bacteria for feeding. Background technique [0002] Active lactic acid bacteria agent has the functions of promoting gastrointestinal digestion and absorption, regulating intestinal microecological balance, improving body immunity, disease prevention and health care, etc. At the same time, lactic acid bacteria preparations are widely used in industries such as food health care, animal feed, aquaculture, microbial fermentation, and environmental protection because of their advantages such as natural edible, non-toxic and side effects, safe and reliable, and easy large-scale fermentation. However, currently commercially available lactic acid bacteria are mostly liquid bacterial preparations, which have a single dosage form and a relatively short shelf life, while solid lactic acid bacteria prepara...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23K10/18C12R1/23C12R1/245C12R1/225C12R1/25C12R1/01
CPCA23K10/18C12N1/04C12N1/20A23V2400/157A23V2400/125A23V2400/123A23V2400/175A23V2400/113A23V2400/169
Inventor 赵林岳秋林李昆仑李宝君孙欣
Owner SHANDONG CHENZHANG BIOTECHNOLOGY CO LTD
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