Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for synthesizing salidroside and icarisid D2 through biotransformation and application thereof

A technology for icariside and salidroside, which is applied in the field of biotransformation and synthesis of salidroside and icariside D2, can solve the problems of many by-products, high loss of raw materials, high cost, etc., and achieve the required Short time, high conversion rate, and the effect of reducing loss

Active Publication Date: 2019-04-26
HUNAN UNIV OF CHINESE MEDICINE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main function of β-glucosidase in organisms is to catalyze the hydrolysis of glycosidic bonds, and the synthesis of salidroside uses its reverse reaction, which is limited by thermodynamic control and reaction balance, and the yield is low
[0006]Salidroside can also be synthesized by chemical method, but because the chemical synthesis process needs to go through multi-step protection and deprotection measures, the process is complicated and costly, and the by-products are more Many and difficult to separate, more harmful to the environment, so no large-scale production
[0007] Although there are reports in the prior art about adding specific raw materials to the water body, soil or leaves where plants grow, and generating salidroside through biotransformation, this The addition method consumes a lot of raw materials, most of which cannot be absorbed and utilized, and the conversion rate of salidroside is low
The content of Icariside D2 in Rhodiola rosea is lower than that of salidroside, and there is no report of any synthesis method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for synthesizing salidroside and icarisid D2 through biotransformation and application thereof
  • Method for synthesizing salidroside and icarisid D2 through biotransformation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Leonurus suspension cell culture: Take 5g of the purified and vigorously growing Leonurus loose callus, insert it into 100mL culture medium (MS medium+0.5mg / L 2,4-dichlorophenoxyacetic acid+2.0mg / L6- benzylaminoadenine) in a 250 mL shake flask. According to the new solution: old solution = 3:1, change the solution every 5 days. In the early stage of culture, remove large cell clusters each time the medium is changed. After cultivating for a period of time, when there are sufficient small cell clusters, pass the suspended cell liquid through a 50-mesh sieve when changing the medium, harvest the filtrate, let it stand for a while until the cells sink, and discard the upper part with a ratio of new solution to old solution of 3:1. solution, supplemented with new solution, and continued the shaker culture. After repeatedly passing through a 50-mesh sieve for a period of time, the motherwort suspension cell line was established. Thereafter, pass through a 50-mesh sieve...

Embodiment 2

[0029] 1. Motherwort suspension cell culture: the culture method is the same as in Example 1.

[0030]Transformation method: add an aqueous solution of p-hydroxyphenylethanol to the transformation system of motherwort suspension cells to make the initial concentration 5mmol / L, and harvest 24h and 48h after the addition, and harvest twice in total.

[0031] A blank group is set at the same time, the difference between the blank group and the present embodiment is only that the blank group does not add p-hydroxyphenylethanol aqueous solution.

[0032] 2. Salidroside and Icariside D 2 The detection of: detection method is with embodiment 1.

[0033] It can be seen from the detection results that the total conversion rate of salidroside in the Leonurus suspension cell transformation system obtained in this example (29.78% in the cell + 5.71% in the culture medium) in 24 hours was 35.49%, and icariside D 2 The total conversion rate (5.03% in cells + 1.02% in culture medium) was 6...

Embodiment 3

[0035] 1. Motherwort suspension cell culture: the culture method is the same as in Example 1.

[0036] Transformation method: add an aqueous solution of p-hydroxyphenylethanol to the transformation system of motherwort suspension cells to make the initial concentration 7mmol / L, harvest 1h and 48h after the addition, and harvest twice in total.

[0037] A blank group is set at the same time, the difference between the blank group and the present embodiment is only that the blank group does not add p-hydroxyphenylethanol aqueous solution.

[0038] 2. Salidroside and Icariside D 2 The detection of: detection method is with embodiment 1.

[0039] It can be seen from the detection results that the total conversion rate of salidroside in the Leonurus suspension cell transformation system obtained in this example (3.40% in the cell + 1.09% in the culture medium) in 1h was 4.49%, and icariside D 2 The total conversion rate (1.08% in cells + 0.76% in culture medium) was 1.84%; the to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biotransformation, and discloses a method for synthesizing salidroside and icarisid D2 through biotransformation and application thereof. The method ischaracterized in that p-hydroxyphenylethyl alcohol is added into a culture solution containing motherwort suspension cells, after a certain transformation time, the motherwort suspension cells containthe salidroside and the icarisid D2, so that the nutrient solution also contains the salidroside and the icarisid D2. According to the method for synthesizing the salidroside and the icarisid D2 through biotransformation and the application thereof, the p-hydroxyphenylethyl alcohol is directly added into the culture solution containing the motherwort suspension cells and is converted and synthesized into the salidroside and the icarisid D2, and therefore the loss of the p-hydroxyphenylethyl alcohol is greatly reduced, the conversion rate is high, the required time for culture of the motherwort suspension cells is short, the salidroside and the icarisid D2 are easy to obtain, and the operation is very simple, convenient and safe.

Description

technical field [0001] The invention relates to the technical field of biotransformation, in particular to a biotransformation method for synthesizing salidroside and icariside D 2 methods and applications. Background technique [0002] Salidroside (salidroside) is the most important medicinal ingredient of Crassulaceae (Crassulaceae) Rhodiola (Rhodiola L.) plant, which has anti-fatigue, enhanced immunity, cardiovascular protection, anti-hypoxia, anti-aging, Protect nerve cells, protect the liver, anti-oxidation, anti-tumor, anti-radiation and other specific effects. It has extremely important application value in military, aerospace, sports and health care medicine. In recent years, there are more and more types of products such as pharmaceuticals, beverages, food and cosmetics produced with it as the main raw material, and people's demand for salidroside is increasing. [0003] Rhodiola plants are the main source of extracting salidroside, but wild resources are scarce,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L29/00A61K36/533
CPCA23L29/03A23L33/00A61K36/533A23V2002/00A23V2200/30
Inventor 谭朝阳罗月芳曾晓艳王智刘塔斯徐德宏孟蕾江灵敏
Owner HUNAN UNIV OF CHINESE MEDICINE