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Method for preparation of deodorant composite bio-enzyme

A technology of compound biological enzyme and culture medium, applied in the field of preparation of deodorizing compound biological enzyme, can solve the problems of high operating cost, environmental pollution, large investment, etc., and achieve the effects of eliminating pollutants, abundant sources and simple equipment.

Inactive Publication Date: 2019-04-30
南昌市医疗废物处置中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the investment is large and the operating cost is relatively high, especially the products after the chemical reaction may cause new uncontrollable environmental pollution

Method used

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  • Method for preparation of deodorant composite bio-enzyme
  • Method for preparation of deodorant composite bio-enzyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of embodiment 1 deodorizing composite biological enzyme

[0028] 1. Culture medium preparation: Prepare a solution of pH 7.0 with 4% corn flour, 3% bean cake flour, 3.2% bran, 1% bran, 0.4% disodium hydrogen phosphate and 0.03% potassium dihydrogen phosphate, and use it as a solution in yeast Protease fermentation medium 1, inoculate yeast in medium 1 and cultivate it at 35 degrees to obtain yeast liquid, namely liquid 1;

[0029] Glucose 10%, yeast extract 1%, agar 1.5% and distilled water are prepared into a solution of pH 6.8 as acetic acid bacteria medium 2, and acetic acid bacteria are inoculated in medium 2 and cultivated at 31 degrees to obtain acetic acid bacteria liquid. i.e. liquid two;

[0030] Prepare 10g of glucose, 7.5g of yeast extract, 7.5g of peptone, 100ml of tomato juice, 0.5ml of Tween-80 and distilled water to make 900ml of a pH7.0 solution, which is used as lactic acid bacteria medium 3, and inoculate lactic acid bacteria in...

Embodiment 2

[0040] Example 2 Deodorization test

[0041] Test method: Dilute the deodorant compound biological enzyme of the present invention according to the ratio of 1:1500 to obtain the diluted deodorant of the present invention, add the same amount of deodorant of the present invention into two automatic spray devices, and then The automatic spraying device was placed in the medical waste storage room and the public toilet respectively for continuous automatic spray deodorization, and the deodorization rate in the medical waste storage room and the public toilet was tested after 5 and 10 minutes respectively.

[0042] The test results are as follows:

[0043] Table 1

[0044] Place

time (min)

Deodorization rate (%)

time (min)

Deodorization rate (%)

Medical waste storage room

5

86.3%

10

96.7%

public toilet

5

87.82%

10

98%

[0045] Table 1 shows the deodorization results of the deodorant of the present invention on m...

Embodiment 3

[0056] Example 3 Acute Oral Toxicity Test

[0057] Test method: "Safety and Technical Specifications for Cosmetics (2015)" China Food and Drug Administration

[0058] Test sample: deodorant of the present invention

[0059] 1. Experimental animals: KM mice, SPF grade, weighing 18-22g.

[0060] 2. Testing environment: SPF animal room, room temperature 20-22 degrees, relative humidity 45-60%.

[0061] 3. Test procedure: Randomly select animals into groups, and the test animals are fasted overnight for 16 hours. Using the one-time limited method, the intragastric dosage is 5.0g / kg, and the intragastric capacity is calculated as 20mg / kg.BW. Observe the animal's general state, poisoning state, behavior change state and death situation twice a day, and weigh once every 3 days. Case observations were made on animals that died of poisoning and animals that survived 14 days after exposure.

[0062] The test results are as follows:

[0063] table 3

[0064]

[0065] Table 3 sh...

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Abstract

A method for preparation of a deodorant composite bio-enzyme includes the steps: preparation of culture media: obtaining a culture medium 1, a culture medium 2 and a culture medium 3; raw material treatment: selecting and cleaning rotten vegetable leaves and peel; material preparation: obtaining a raw material 1, a raw material 2 and a raw material 3; sterilization: putting a fermentation tank into acetic acid, and disinfecting; and fermentation: firstly, carrying out first-stage fermentation, also known as saccharification, then carrying out second-stage fermentation, also known as acetification, followed by carrying out lactic acid bacteria fermentation stage, also known as ripening, next carrying out metabolism stage, mainly producing a variety of proteins, amino acids and other complexcomponents, and finally continuing to ferment until the pH value of fermentation broth drops to 3.5 or less, and finishing the fermentation when no other peculiar smell exists. The invention providesan all-natural broad-spectrum composite bio-enzyme deodorant with simple method, low cost, no toxicity, harmlessness and no secondary pollution, and a preparation method thereof.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a preparation method of a deodorizing compound biological enzyme. Background technique [0002] At present, the commonly used deodorants mainly include the following types of physical deodorants, chemical deodorants, microbial deodorants, plant-based deodorants and compound deodorants. Physical deodorants are deodorized by physical methods, using the physical properties of deodorants or odors, without changing the results of odor components, but only changing the local concentration, or relative concentration, of odors. The common ones are adsorption deodorants, masking deodorants, etc. [0003] The disadvantage is that it is difficult to completely change the composition of the odorous gas, and there is still a certain degree of damage to humans, animals, equipment and the environment. Chemical deodorants use oxidation, reduction decomposition, neutralization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/60C12N1/20C12N1/16C12P39/00A61L9/01A61L101/54
CPCA61L9/01C12N1/16C12N1/20C12N9/60C12P39/00A61L2209/21
Inventor 方平平张育红刘冰媛李拓宋忠伟
Owner 南昌市医疗废物处置中心有限公司