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Method for increasing yield of dibasic acid fermentation

The technology of dibasic acid and microbial fermentation method is applied in the field of improving the fermentation yield of long-chain dibasic acid, can solve the problems of raw material consumption, low conversion rate of fermented alkane and high production cost, and achieves reduction of unit consumption of alkane and conversion of fermented alkane. The effect of high rate and fast acid production

Inactive Publication Date: 2019-04-30
陈凯南
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Although the α, ω-oxidation ability of domestic and foreign production strains is relatively strong, and the acid production can reach up to 150g / L, but one of the most important problems in the process of producing long-chain dibasic acids is the low conversion rate of fermented alkanes. The molar conversion rate of long-chain dibasic acid is only 35%, thus causing a large consumption of raw materials and high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0021] The method for improving the fermentation yield of long-chain dibasic acid comprises the following steps:

[0022] (1) Take an inoculation loop of Candida tropicalis, spread it on the solid slant medium of a large test tube of 20x200mm, and cultivate it at 30°C for 48 hours. The slant medium is a solid slant medium (g / L): yeast extract 5, white sugar 10, peptone 5 and agar 15.

[0023] (2) Transfer the bacteria cultured in step (1) into a 500mL Erlenmeyer shaker flask filled with 15mL of seed medium, and cultivate for 40 hours at 30°C on a rotary shaker at 180 rpm.

[0024] Seed medium (g / L): White sugar 5, potassium dihydrogen phosphate 8, yeast extract 1.5, corn steep liquor 1.5, urea 1.8, 200# heavy wax oil 30ml / L, prepared with tap water, natural pH.

[0025] (3) In a 500mL Erlenmeyer shaker flask containing 15mL of fermentation medium, add 5mI of the above seed solution, and at the same time add 0.5mmol / L inhibitor chlorpromazine hydrochloride, and make 3 parallel...

Embodiment 2

[0029] According to the method of Example 1, replace chlorpromazine hydrochloride with inhibitor phenobarbital sodium, add when inoculating shake flask, concentration is 1mmol / L, fermentation substrate is nC13, and addition is 3ml. The residual hydrocarbon is 0 and the fermentation is over. The dibasic acid is measured by the method of Example 1, and the conversion rate of the fermented alkane is obtained through calculation. ) reached 96.8wt.%, the total acid content (neutralization titration) was 99.1wt.%, and the molar conversion of alkane reached 58.0%.

Embodiment 3

[0031] According to the method of embodiment 1, replace chlorpromazine hydrochloride with inhibitor acrylic acid, and add when cell culture 28 hours, concentration is 0.05mmol / L, fermentation substrate is nCl4, and addition is 3ml. The residual hydrocarbon is 0 and the fermentation ends. After the fermentation, the method of Example 1 was used to measure the dibasic acid, and the conversion rate of the fermented alkane was calculated. As a result, the amount of acid produced was 162.5g / L, and 1.9013g of tetradecanedioic acid was extracted, and the monoacid content (GC) reached 97.5 wt.%, the total acid content (neutralization titration) was 99.4wt.%, and the molar conversion of alkane reached 64%.

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Abstract

The invention relates to a method for increasing a yield of dibasic acid fermentation, which uses C10-C18 n-alkane as a raw material and employs a microbial fermentation method. The method is characterized by the addition of an alpha-oxidative decarboxylation inhibitor and / or a beta-oxidation inhibitor during fermentation production is used to attenuate the alpha-oxidative decarboxylation and beta-oxidicability of the strain, the alpha-oxidative decarboxylation inhibitor is one or a mixture of chlorpromazine hydrochloride, sodium phenobarbital, acrylic acid or polyacrylic acid, and the beta-oxidation inhibitor is one or a mixture of thioglycolic acid, sodium thioglycolate, ranolazine, ranolazine dihydrochloride or rice koji. The method has the beneficial effects that in the process of producing dibasic acid with the corresponding carbon number by n-alkane fermentation, the inhibitor is used to weaken the alpha-oxidative decarboxylation and beta-oxidation ability of the strain, and reduces the unit consumption of the alkane, and the alkane fermentation conversion rate is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of chemical product preparation, and relates to a method for increasing the fermentation yield of long-chain dibasic acids. Background technique [0002] Long-chain dibasic acid is an important fine chemical raw material, which can be used in the synthesis of spices, hot-melt adhesives, engineering plastics and liquid crystals, etc., and is widely used. [0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation, and the special function of microbial double-end oxidation is used to ferment normal alkanes to produce long-chain dibasic acids. According to the mechanism study of microbial oxidation of alkanes to long-chain dibasic acids, the main reaction is α, ω-oxidation, and the side reactions are α-oxidative decarboxylation and β-oxidation, and the long-chain dibasic acids produced can be further oxidatively degraded . Although the α, ω-oxidation ability of domestic and for...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/74
CPCC12P7/6409
Inventor 陈凯南
Owner 陈凯南
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