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Regenerated tissue matrix microparticles for micro-plastic surgery, preparation method and application

A technology for tissue regeneration and micro-shaping, applied in medical science, prostheses, etc., can solve the problems of increasing the economic pressure of users, the difficulty of operation by clinicians, the short duration of maintenance effect, and the tendency to appear uneven, so as to achieve easy cell adhesion and emigration, low usage, and the effect of avoiding burning damage

Active Publication Date: 2022-02-15
北京颢美细胞基因生物技术有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0013] Currently commonly used fillers can achieve the purpose of smoothing wrinkles, improving facial defects, and sculpting perfect skin by increasing the capacity of dermal layer tissue. The maintenance time is short or due to the natural defects of heterogeneous collagen (degradation speed is faster, prone to unevenness), increasing the economic pressure of the user and the difficulty of operation for the clinician

Method used

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preparation example Construction

[0059] The preparation method of the regenerated tissue matrix microparticles proposed by the present invention comprises the steps of:

[0060] 1) Put the allograft skin raw material into the glycerin solution of alkali, soak and vibrate to obtain the semi-finished product A;

[0061] 2) Remove the epidermis of the semi-finished product A, put it into a surfactant solution, and perform ultrasonic soaking treatment to obtain the semi-finished product B;

[0062] 3) Cut the semi-finished product B into small skins, immerse it in liquid nitrogen for 3-10 minutes, and take it out as semi-finished product C;

[0063] 4) Under the action of high-speed rotating centrifugal force, the semi-finished product C is cut with a rotary knife to obtain the semi-finished product D;

[0064] 5) Soak the semi-finished product D in the D-amino acid solution, and separate the solid and liquid to obtain the semi-finished product E;

[0065] 6) The semi-finished product E is made into a prefilled...

Embodiment 1-5

[0069] 1) Put the allograft skin raw material into the glycerol solution of alkali, soak it at 25-35°C for 1-10 hours and shake it to get the semi-finished product A;

[0070] 2) After removing the epidermis of the semi-finished product A, wash it with sterile pure water for 0.1-5.0 minutes, put it into the surfactant solution, and ultrasonicate it for 1-5 minutes at an ultrasonic frequency of 20-45KHz and an ultrasonic temperature of 1-20°C. Soak 1~4h, change the surfactant solution of new; Repeat the treatment of ultrasonic and immersion, get semi-finished product B (specifically, embodiment 1 is 2.5h four times. Embodiment 2 is 2h four times. Embodiment 3 is 2.5h Secondary. Embodiment 4 is 2h once. Embodiment 5 is 1h once).

[0071] 3) Optionally, the semi-finished product B is rinsed with sterile pure water for 0.1-5.0 min, and put into 0-0.6% genipin aqueous solution (the pH of the genipin solution at this concentration is 5-7) for cross-linking, Rinse with sterile pure ...

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Abstract

The invention provides a regeneration tissue matrix particle for micro-plastic surgery, a preparation method and an application. The residual amount of DNA in the regenerated tissue matrix microparticles is below 2.0ng / mg, the particle diameter of the regenerated tissue matrix microparticles is below 1.2mm, and the D50 is between 0.018-1.0mm. The particle size distribution of the regenerative tissue matrix particles is narrow, easy to inject, and the preparation process of the present invention retains the three-dimensional framework structure of the regenerative tissue matrix, making it easier for cells to adhere and move in, which is beneficial to the growth of capillaries, and allows the filled parts to rebuild blood. It can be directly used for microplastic injections, and can also be used to repair tissue damage caused by multiple injections of hyaluronic acid and removal of prostheses. The preparation method adopted in the present invention can remove cells quickly and efficiently, so that the obtained regenerated tissue matrix has a lower amount of DNA residue.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a regeneration tissue matrix particle for tissue repair, a preparation method and application thereof. Background technique [0002] In recent years, with the rapid development of social economy, people have a higher pursuit of beauty, which has greatly promoted the rapid development of minimally invasive injection regenerative medicine technology. [0003] Injectable fillers (also known as Dermal Filler, Soft Tissue Filler) required for minimally invasive injection technology are mainly used for trauma, congenital defects, acquired traumatic defects, congenital deformities and other defects. Plastic surgery and restorative treatment, including facial micro-plastic surgery, ear micro-plastic surgery, breast augmentation, filling and repair of joint soft tissue damage, orthognathic, supplementary orthodontic repair surgery, and has unique advantages such as small tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 莫丽影孙善凤石卫华王姣王正元
Owner 北京颢美细胞基因生物技术有限公司
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