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A kind of fermentation preparation method of two-step Cordyceps militaris enzyme combined with compound enzymolysis

The technology of compound enzymolysis and Cordyceps militaris is applied in the field of research and development of fermented beverages, which can solve the problems of long fermentation period and various raw materials, and achieve the effects of short fermentation period, unique flavor and improving fermentation efficiency.

Active Publication Date: 2022-07-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent also utilizes enzymatic hydrolysis and inoculation fermentation technology, due to the variety of raw materials used, the fermentation cycle is still long, and it needs to be fermented at 28-30°C for 22-24 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: a kind of fast fermentation method of Cordyceps militaris enzyme combined with low ratio cellulase / pectinase compound enzymolysis

[0032] After the dry sample of Cordyceps militaris is removed, use a high-speed pulverizer to pulverize, and pass through a 100-mesh sieve to obtain Cordyceps militaris powder; The Cordyceps militaris powder and water are dissolved in 1:10, and stir with a high-speed homogenizer to obtain Cordyceps militaris pulp; 300g of Cordyceps militaris slurry, add 150g of brown sugar, and add 550g of distilled water to quantify to 1kg, fully stir to dissolve the brown sugar completely, and then go through pasteurization treatment at 85°C for 10min to obtain the fermentation substrate of Cordyceps militaris; Wait for the fermentation substrate to cool down to 40-45°C, adjust the pH to 4.80±0.10, add 0.4% cellulase and 0.6% pectinase, and inoculate 1 g of compound lactic acid bacteria powder, so that compound enzymatic hydrolysis and lactic...

Embodiment 2

[0035] Embodiment 2: a kind of fast fermentation method of the Cordyceps militaris enzyme combined with equal proportion cellulase / pectinase complex enzymolysis

[0036] After the dry sample of Cordyceps militaris is removed, use a high-speed pulverizer to pulverize, and pass through a 100-mesh sieve to obtain Cordyceps militaris powder; The Cordyceps militaris powder and water are dissolved in 1:10, and stir with a high-speed homogenizer to obtain Cordyceps militaris pulp; 300g of Cordyceps militaris slurry, add 150g of brown sugar, and add 550g of distilled water to quantify to 1kg, fully stir to dissolve the brown sugar completely, and then go through pasteurization treatment at 85°C for 10min to obtain the fermentation substrate of Cordyceps militaris; Wait for the fermentation substrate to cool down to 40~45℃, adjust the pH to 4.80±0.10, add 0.5% cellulase and 0.5% pectinase, and inoculate 1g of compound lactic acid bacteria powder, so that compound enzymatic hydrolysis an...

Embodiment 3

[0039] Embodiment 3: a kind of fast fermentation method of the cordyceps militaris enzyme combined with high proportion cellulase / pectinase compound enzymolysis

[0040] After the dry sample of Cordyceps militaris is removed, use a high-speed pulverizer to pulverize, and pass through a 100-mesh sieve to obtain Cordyceps militaris powder; The Cordyceps militaris powder and water are dissolved in 1:10, and stir with a high-speed homogenizer to obtain Cordyceps militaris pulp; 300g of Cordyceps militaris slurry, add 150g of brown sugar, and add 550g of distilled water to quantify to 1kg, fully stir to dissolve the brown sugar completely, and then go through pasteurization treatment at 85°C for 10min to obtain the fermentation substrate of Cordyceps militaris; Wait for the fermentation substrate to cool down to 40~45℃, adjust the pH to 4.80±0.10, add 0.6% cellulase and 0.4% pectinase, and inoculate 1g of compound lactic acid bacteria powder, so that compound enzymatic hydrolysis an...

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Abstract

A two-step fermentation preparation method of Cordyceps militaris enzyme combined with compound enzymolysis belongs to the technical field of research and development of fermented beverages. In the present invention, the preparation of cordyceps militaris powder and cordyceps militaris pulp, preparation of cordyceps militaris fermentation substrate, compound enzymolysis synergistic lactic acid bacteria fermentation, yeast fermentation and other processes are used to obtain products through centrifugation, filtration, preparation, sterilization and filling. The present invention utilizes compound enzymatic hydrolysis to synergize lactic acid bacteria fermentation, and then undergoes secondary fermentation by yeast, so that the entire fermentation process can be quickly completed within 2 to 3 days, and the obtained cordyceps militaris enzyme is sweet and sour, unique in flavor and good in suspension stability. , and can well retain the important active ingredient of cordycepin: the dissolution rate of cordycepin in cordyceps powder can reach 78.21%, and the preservation rate of dissolved cordycepin can reach 97.78%. It has certain antioxidant properties and improves immunity. Function, in line with modern people's pursuit of healthy, delicious high-quality beverages.

Description

technical field [0001] The invention relates to a two-step fermentation preparation method of Cordyceps militaris enzyme combined with compound enzymolysis, in particular to a rapid fermentation method of using brown sugar as a carbon source, utilizing compound enzymolysis to promote lactic acid bacteria fermentation, and then inoculating yeast for secondary fermentation. The fermentation method belongs to the technical field of research and development of fermented beverages. Background technique [0002] Cordyceps militaris is a parasitic fungus that has been listed as a new resource food in 2009. It contains a variety of nutrients, such as protein and amino acids, Cordyceps polysaccharides, cordycepic acid (D-mannitol), cordycepin and superoxide disproportionation Enzyme (SOD) etc. Ancient records and modern studies have shown that the medicinal value of Cordyceps militaris is similar to that of Cordyceps sinensis and comparable to that of ginseng and velvet antler. Its ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L2/38A23L2/84A23L33/00
CPCA23L2/84A23L2/382A23V2400/125A23V2400/113A23V2400/531A23V2400/169
Inventor 张慜劳艳艳阳武雄易思富侯丽华郭小玲
Owner JIANGNAN UNIV
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