Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of transformation method of rare ginsenoside

A kind of ginsenoside, a rare technology, applied in the field of high-efficiency biotransformation of rare ginsenosides, high-speed countercurrent chromatography reactor, can solve the problems of cumbersome steps, undisclosed reverse micellar system, etc., achieve high flexibility, reduce product inhibition effect, The effect of easy operation

Active Publication Date: 2020-12-01
SHANDONG ANALYSIS & TEST CENT
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, enzymes are usually added to ginsenosides to complete the biotransformation reaction, and then the rare ginsenosides after the reaction are separated by high-speed countercurrent chromatography. A technical solution directly used as a rare ginsenoside bioenzyme conversion reactor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of transformation method of rare ginsenoside
  • A kind of transformation method of rare ginsenoside
  • A kind of transformation method of rare ginsenoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Preparation of reverse micellar solvent system and preparation of sample solution

[0054] Take by weighing certain quality sodium dioctyl succinate sulfonate (AOT), triton (Triton) X-100, dissolve with isooctane, settle to a certain volume, be mixed with total surfactant concentration and be 0.10 mol / L AOT / Triton X-100 / iso-octane solution. Prepare 50 μmol / L β-glucosidase solution with phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 5, in 0.10 mol / L AOT / Add a certain volume of β-glucosidase solution to the Triton X-100 / isooctane reverse micelles system to prepare a certain volume of enzyme-reverse micelles system. The above-mentioned enzyme-reverse micelles system was subjected to saturation partitioning with phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 5, and the upper and lower phases were separated. The upper phase is a stationary phase containing enzyme solution, and 1.2 g / L of ginsenoside Rb1 substrate...

Embodiment 2

[0063] 1. Preparation of reverse micellar solvent system and preparation of sample solution

[0064] Take by weighing certain quality sodium dioctyl succinate sulfonate (AOT), triton (Triton) X-100, dissolve with isooctane, settle to a certain volume, be mixed with total surfactant concentration and be 0.10 mol / L AOT / Triton X-100 / iso-octane solution. Prepare a 50 μmol / L β-glucosidase solution with a phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 5, and add a certain volume of β-glucosidase solution to prepare a certain volume of enzyme-reverse micelles system. The above-mentioned enzyme-reverse micelles system was subjected to saturation partitioning with phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 5, and the upper and lower phases were separated. The upper phase is a stationary phase containing enzyme solution, and 1.5 g / L of ginsenoside Re substrate solution is added below, and part of the blank lower phase solut...

Embodiment 3

[0073] 1. Preparation of reverse micellar solvent system and preparation of sample solution

[0074] Take by weighing certain quality sodium dioctyl succinate sulfonate (AOT), triton (Triton) X-100, dissolve with isooctane, settle to a certain volume, be mixed with total surfactant concentration and be 0.10 mol / L AOT / Triton X-100 / iso-octane solution. Prepare 50 μmol / L β-glucosidase solution with phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 5, in 0.10 mol / L AOT / Add a certain volume of β-glucosidase solution to the Triton X-100 / isooctane reverse micelles system to prepare a certain volume of enzyme-reverse micelles system. The above-mentioned enzyme-reverse micelles system was subjected to saturation partitioning with phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 5, and the upper and lower phases were separated. The upper phase is a stationary phase containing enzyme solution, and 1.0 g / L ginsenoside Rg1 substrate so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of ginsenoside biological conversion, in particular to a conversion method of rare ginsenoside, and aims at solving the problems that instruments of an enzyme membrane converter and the like are mostly adopted when ginsenoside is converted into the rare ginsenoside in the prior art, so that the recovery is difficult, most enzymes are insoluble to organic solvents, which is adverse to enzymatic hydrolysis reaction. In order to solve the technical problems, a high-speed countercurrent chromatographic reactor based on a reverse micelle system is provided and used for the efficient biological conversion of the rare ginsenoside. The reverse micelle system can protect the activity of enzymes to improve the solubility in an organic medium and improve the enzymatic efficiency, a high-speed countercurrent chromatography is taken as the reactor to separate the generated rare ginsenoside by using the separation coefficient difference of products and substrates,different rare ginsenosides can be obtained by adjusting the rotation rate, and the method has significant meanings when applied to industrial production.

Description

technical field [0001] The patent of the present invention relates to the field of biotransformation of ginsenosides, in particular to a high-speed countercurrent chromatography reactor based on a reverse micellar system, which is used for high-efficiency biotransformation of rare ginsenosides. Background technique [0002] The main active substances of Araliaceae plants such as ginseng, American ginseng, Panax notoginseng are ginsenosides, which have various pharmacological effects such as anti-tumor, anti-aging, improving immunity and increasing angiogenesis. However, primary ginsenosides have the characteristics of high polarity and high molecular weight, and are not easily absorbed through the intestinal wall mucosa. Due to the long residence time of the primary ginsenosides in the intestine, some glycosides are transformed by the enzymes secreted by the specific intestinal flora into rare ginsenosides such as ginsenoside CK and ginsenoside Rg3, which pass through the in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00C12M1/40
Inventor 王岱杰耿岩玲崔莉宋祥云李景超
Owner SHANDONG ANALYSIS & TEST CENT