Sea cucumber peptide and production method thereof

A production method and technology of sea cucumber peptide, applied in the field of sea cucumber peptide and its production, can solve the problems of low enzymatic hydrolysis efficiency, cumbersome steps, easy moisture absorption and agglomeration, etc., achieve low free amino acid content, improve enzymatic hydrolysis efficiency, and not easy to absorb damp caking effect

Active Publication Date: 2019-05-17
舜甫科技(固安)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the problems and shortcomings of the above two invention patent applications are: the enzymatic hydrolysis efficiency of the enzyme used is low, the enzymatic hydrolysis time is long, the amount of enzyme added is large, the optimum pH value and optimum temperature need to be adjusted for enzymatic hydrolysis, and the operation is complicated , adjusting the optimum pH value of enzymolysis will bring in a large amount of inorganic ions, and controlling the optimum enzymolysis temperature consumes a lot of energy; two membrane filtrations with ultrafiltration membrane and nanofiltration membrane are required, and the role of ultrafiltration membrane is as follows: To intercept macromolecular substances, the role of nanofiltration membrane is desalination and concentration, the steps are cumbersome, and the membrane core used is organic material, which is not resistant to acid and alkali, high temperature and high pressure, small flux, short service life, and inconvenient cleaning; The water resolubility of the prepared sea cucumber peptide powder is poor, and it is easy to absorb moisture and agglomerate during storage

Method used

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  • Sea cucumber peptide and production method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] (1) Raw material pretreatment: clean the sea cucumber body wall, add 1 times the quality of water, and mash the tissue into sea cucumber homogenate;

[0045] (2) Heat treatment: heat the sea cucumber homogenate in hot water at 100°C for 30 minutes, then cool it down to 50-55°C to obtain a denatured sea cucumber solution;

[0046] (3) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the denatured sea cucumber solution, mix evenly, and enzymatically hydrolyze for 1 hour under natural conditions without adjusting the pH value and controlling the temperature , to prepare a sea cucumber peptide solution; the high-efficiency compound enzyme preparation is composed of the following components in mass percentage: 30% compound protease, 30% acid protease, 30% neutral protease, 7% animal proteolytic enzyme and enzyme Activator 3%. The enzyme activator consists of reducing agent dithiothreitol, β-mercaptoethanol and divalent metal ...

Embodiment 2

[0052] (1) Raw material pretreatment: clean the sea cucumber body wall, add 2 times the quality of water, and mash the tissue into sea cucumber homogenate;

[0053] (2) Heat treatment: heat the sea cucumber homogenate in hot water at 100°C for 30 minutes, then cool it down to 50-55°C to obtain a denatured sea cucumber solution;

[0054] (3) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the denatured sea cucumber solution, mix evenly, and enzymatically hydrolyze for 1 hour under natural conditions without adjusting the pH value and controlling the temperature , to prepare sea cucumber peptide solution; the high-efficiency compound enzyme preparation is composed of the following components in mass percentage: compound protease 35%, acid protease 27.5%, neutral protease 27.5%, animal proteolytic enzyme 7.5% and enzyme Activator 2.5%. The enzyme activator consists of reducing agent β-mercaptoethanol and divalent metal ion Mg 2+...

Embodiment 3

[0060] (1) Raw material pretreatment: clean the sea cucumber body wall, add 3 times the quality of water, and mash the tissue into sea cucumber homogenate;

[0061] (2) Heat treatment: heat the sea cucumber homogenate in hot water at 100°C for 30 minutes, then cool it down to 50-55°C to obtain a denatured sea cucumber solution;

[0062] (3) Enzymolysis: Add 0.1% (w / w) high-efficiency compound enzyme preparation of raw material weight to the denatured sea cucumber solution, mix evenly, and enzymatically hydrolyze for 1 hour under natural conditions without adjusting the pH value and controlling the temperature , to prepare a sea cucumber peptide solution; the high-efficiency compound enzyme preparation is composed of the following components in mass percentage: 40% compound protease, 25% acid protease, 25% neutral protease, 8% animal proteolytic enzyme and enzyme Activator 2%. The enzyme activator consists of reducing agent dithiothreitol and divalent metal ion Mg 2+ , Zn 2+...

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Abstract

The invention belongs to the field of sea cucumber peptide, and discloses a sea cucumber peptide and a production method thereof. The method comprises the steps that water is added to a sea cucumber raw material, homogenated and heated, high-efficient compound enzymic preparation enzymolysis is conducted under the conditions that the pH value is not adjusted and the temperature is not controlled,components which are hard to dissolve and easily absorb dampness are filtered and removed by an inorganic nanofiltration ceramic membrane, high-temperature and high-pressure processing is conducted, and sea cucumber peptide powder is prepared after concentration and drying are conducted. The method has the advantages that the enzymolysis pH value does not need to be adjusted, the enzymolysis temperature does not need to be controlled, the enzymolysis time is short, the enzyme adding amount is less, the molecular weight of enzymolysis products is low and concentrated, and the water resolubilityof prepared sea cucumber peptide powder is excellent, and dampness is not easily absorbed and blocks are not easily formed in the storage process.

Description

technical field [0001] The invention belongs to the field of sea cucumber peptides, in particular to a sea cucumber peptide and a production method thereof. Background technique [0002] Sea cucumbers, belonging to the class Holothuria, are marine echinoderms that live at seasides up to 8,000 meters high. They have a history of more than 600 million years. Sea cucumbers feed on seabed algae and plankton. Sea cucumbers are covered with thorns and are widely distributed in oceans all over the world. There are many kinds of sea cucumbers along the coast of the South China Sea, and there are more than 20 kinds of sea cucumbers available for consumption. Sea cucumber is as famous as ginseng, bird's nest and shark's fin, and is one of the eight treasures in the world. Sea cucumber is not only a precious food, but also a valuable medicinal material. According to the records in "Compendium of Materia Medica": Sea cucumbers are sweet and salty, tonify the kidneys, benefit the esse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23J3/34
CPCY02P20/10
Inventor 丁旭初
Owner 舜甫科技(固安)有限公司
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