Ecological restoration method for opencast coal mine dump

A technology for open-pit coal mines and ecological restoration, applied in the direction of microbial-based methods, biochemical equipment and methods, and horticultural methods, can solve the problems of cumbersome operation of non-biological restoration methods, low resistance stability, and few soil microorganisms, etc., to achieve Improve the survival rate and mycorrhizal infection rate, improve the survival rate, and restore the effect of soil fertility

Inactive Publication Date: 2019-05-21
CHINA SHENHUA ENERGY CO LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As mentioned above, the existing problems in the treatment of mine dumps are: the non-biological remediation methods used are cumbersome and costly, and cannot completely solve the problem of soil environment deterioration in mine dumps; the traditional bioremediation technology, which uses The way of tra...

Method used

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  • Ecological restoration method for opencast coal mine dump
  • Ecological restoration method for opencast coal mine dump
  • Ecological restoration method for opencast coal mine dump

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of liquid ectomycorrhizal agent

[0034] 1. Recovery of Suillus luteus: take out the test tube slant strain Suillus luteus stored in the refrigerator at 4°C, slow down the bacteria for 1 day at room temperature, pick the hyphae at the edge of the test tube slant and transfer to On the Pachlewski plate medium, place it in a constant temperature incubator at 25°C for dark culture for 4-5 days. After the mycelium grows, pick the thick mycelium and transfer it to the Pachlewski plate medium, and place it in a constant temperature incubator for 25 Cultivate in dark for one week at ℃, and store at 4℃ for later use.

[0035] 2. Preparation of liquid ectomycorrhizal agent: In the ultra-clean workbench, pick the mycelium that grows robustly on the Pachlewski plate and add it to 200mL of MMN liquid medium, place it on a shaker, 25°C, 180rpm / min, shake culture 10 days. The obtained liquid culture of Lactobacillus brown ring was added to a homogenize...

Embodiment 2

[0036] Embodiment 2, liquid ectomycorrhizal agent inoculation experiment

[0037]1. Select Pinus tabulaeformis seeds with plump grains and uniform texture, soak them in 10% NaClO solution, disinfect them for 15 minutes, and rinse them with distilled water for 3-5 times. The seeds were evenly spread in petri dishes (90 mm in diameter) covered with two layers of filter paper, 50 grains per dish, placed in a constant temperature incubator at 37°C for germination, and an appropriate amount of distilled water was added every day to keep the filter paper moist. After germination, select seedlings that are strong and uniform in growth, and transplant them into flower pots (23cm×18cm×21.5cm) filled with 3kg of soil. The amount of water is 75%, the photoperiod is 14L:10D, the culture temperature is 27°C, and the air humidity is controlled at 60%.

[0038] 2. When the pine seedlings grow to 50-60cm plant height (the crown width is about 15-20cm, the base diameter is about 5-10cm), take...

Embodiment 3

[0040] The maintenance of seedlings after embodiment 3, transplanting

[0041] Water thoroughly every 5 days in the first month, water every 10 days in the second and third months, water every 15 days in the fourth and fifth months, and then do not need artificial watering. The temperature and light intensity can be according to the natural conditions (daily average temperature 15°C, daily average sunlight 7.8h, daily average wind speed 1.4m / s).

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Abstract

The invention discloses an ecological restoration method for an opencast coal mine dump. The method comprises the steps that pinus tabulaeformis seedlings are transplanted into the opencast coal minedump, and a suillus luteus liquid ectotrophic mycorrhiza agent is inoculated onto the pinus tabulaeformis seedlings. By means of the method, the transplanting survival rate of the pinus tabulaeformisseedlings and the mycorrhiza infection rate can be improved, the microbial environment of soil can be improved, and accordingly, the method has great effects on the aspects of promoting reclamation ofvegetation in the dump, ecological restoration and the like.

Description

technical field [0001] The invention belongs to the field of ecological restoration and relates to a method for ecological restoration of an open-pit coal mine dump. Background technique [0002] my country is rich in coal reserves and is the world's largest coal producer and consumer. Coal is the primary energy source in the Inner Mongolia Autonomous Region, and it is home to the world's largest "open-pit coal mine". The mining process of open-pit coal mines has destroyed a large amount of cultivated land, completely changed the soil structure of the mining area, changed the initial conditions of soil nutrients, and caused a sharp decrease in vegetation coverage and biomass. Therefore, the treatment of dumps and the construction of vegetation are one of the important contents of land reclamation in mining areas. [0003] For dumps mainly composed of parent material, coal rock, mineral residues, mudstone and other mixed loose materials, the surface is exposed, the corrosio...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01G7/06C12N1/14C12R1/645
Inventor 郭洋楠贺安民常建鸿陈金包玉英
Owner CHINA SHENHUA ENERGY CO LTD
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