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Preparing method for L-2-aminobutyric acid

A technology of aminobutyric acid and threonine, which is applied in the field of enzyme catalysis, can solve problems not related to the conversion rate of L-2-aminobutyric acid, and achieve the effect of avoiding purification steps and being convenient to use

Pending Publication Date: 2019-05-21
ANHUI HUAHENG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method prepares L-2-aminobutyric acid and separates the product by crystallization, which greatly improves the purity of the product, but it does not involve the conversion rate of L-2-aminobutyric acid.

Method used

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  • Preparing method for L-2-aminobutyric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Add 1200g of threonine, 1200g of ammonium formate, and ammonium dihydrogen phosphate into a 10L reactor, add water and stir to dissolve, then adjust the pH to 7.5 with 20-25% ammonia water, raise the temperature to 30°C, and then add threonine-producing deaminase , Leucine dehydrogenase and formate dehydrogenase sludge, wherein the concentration of phosphate ions is 20mmol / L, the total enzyme concentration in the mixed solution is 6U, and the mixed solution is stirred and reacted at a constant temperature of 30°C for 16h After the reaction is finished, L-aminobutyric acid solution is obtained.

Embodiment 2

[0023] Add 1200g of threonine, 600g of ammonium formate, and diammonium hydrogen phosphate into a 10L reaction kettle, add water and stir to dissolve, then adjust the pH to 7.5 with 20-25% ammonia water, raise the temperature to 30°C, and then add threonine-producing deaminase , Leucine dehydrogenase and formate dehydrogenase sludge, wherein the concentration of phosphate ions is 10mmol / L, the total enzyme concentration in the mixed solution is 4U, and the mixed solution is stirred and reacted at a constant temperature of 30°C for 20h After the reaction is finished, L-aminobutyric acid solution is obtained.

Embodiment 3

[0025] Add 1200g of threonine, 800g of ammonium formate, and sodium pyrophosphate into a 10L reaction kettle, add water and stir to dissolve, then adjust the pH to 7.7 with 20-25% ammonia water, raise the temperature to 30°C, and then add threonine-producing deaminase, Sludge of leucine dehydrogenase and formate dehydrogenase, wherein the concentration of phosphate ions is 15mmol / L, the total enzyme concentration in the mixture is 6U, the mixture is stirred and reacted at a constant temperature of 30°C, and after 18h After the reaction finishes, L-aminobutyric acid solution is obtained.

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Abstract

The invention belongs to the field enzyme catalysis, and particularly relates to a preparing method for L-2-aminobutyric acid. An enzyme catalyst and an enzyme activating agent are added into substrates, wherein the substrates are threonine and ammonium formate, the enzyme catalyst is threonine dehydratase, leucine dehydrogenase and formate dehydrogenase, and the enzyme activating agent is phosphate. According to the method for preparing the L-2-aminobutyric acid, the phosphate is used as the enzyme activating agent and used in cooperation with the leucine dehydrogenase, reaction intermediateproducts are rapidly converted, the catalytic rates of the threonine dehydratase, the leucine dehydrogenase and the formate dehydrogenase are mutually matched, the conversion efficiency of enzyme catalysis are improved, a stacking phenomenon of by-products is avoided, the yield of products is increased, and separation and purification of the products are more facilitated.

Description

technical field [0001] The invention belongs to the field of enzyme catalysis, in particular to a preparation method of L-2-aminobutyric acid. Background technique [0002] L-2-aminobutyric acid is a non-natural chiral amino acid, which can inhibit the transmission of human nerve information, enhance the activity of glucose phosphatase and promote the metabolism of brain cells. It is an important chemical raw material and pharmaceutical intermediate. It has been widely used in drug synthesis, such as antibacterial and anti-tuberculosis drug ethambutol hydrochloride, antiepileptic drugs levetiracetam and buvaracetam, etc. [0003] At present, the production methods of L-2-aminobutyric acid at home and abroad are divided into chemical method and biological enzymatic method. The chemical method is a relatively classic method, mainly including chemical synthesis and chemical resolution. Chemical synthesis is mainly based on n-butyric acid and bromine as starting materials, and ...

Claims

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Application Information

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IPC IPC(8): C12P13/04
CPCY02P20/584
Inventor 郭恒华刘磊张冬竹刘树蓬章晖田宋魁
Owner ANHUI HUAHENG BIOTECH
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