Capillary analysis detection method based on ultrasonic aggregation

A detection method and capillary technology, applied in the field of analytical chemistry, can solve the problems of inconvenience in wide-scale promotion, long-term storage, complicated capillary, etc., and achieve the effect of fast detection speed and low detection limit.

Inactive Publication Date: 2019-05-28
UNIV OF SCI & TECH BEIJING
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Most of the currently known methods of using capillary for detection also require complex pret

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  • Capillary analysis detection method based on ultrasonic aggregation
  • Capillary analysis detection method based on ultrasonic aggregation
  • Capillary analysis detection method based on ultrasonic aggregation

Examples

Experimental program
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Effect test

Embodiment 1

[0037] This embodiment relates to a SERS detection and verification of a capillary analysis and detection device based on ultrasonic aggregation. The nanoparticles used are gold nanorods. The specific verification method is as follows:

[0038] Step 1, construction of capillary-based microfluidic automatic detection ultrasonic device:

[0039] A zinc oxide layer was deposited on the FTO glass substrate by electromagnetic sputtering, and an aluminum film was deposited by chemical vapor phase on the zinc oxide layer. When depositing the aluminum film layer, Kapton tape was used to cover the central part of the zinc oxide layer. The interdigital transducer (IDT) is formed by wiring on the four sides of the aluminum film through traditional photolithography and lift-off technology, and the IDT is connected with the signal generator and signal amplifier through wires.

[0040] The signal generator uses two wires to input the sound wave signal into the signal amplifier, and then the...

Embodiment 2

[0052]This embodiment relates to a fluorescence detection verification of a capillary analysis and detection device based on ultrasonic aggregation. The specific steps of the fluorescence detection are basically the same as those in Example 1, except that:

[0053] The nanoparticles were replaced with polystyrene nanoparticles.

[0054] The step 2 is replaced by DNA modification on the surface of polystyrene nanoparticles, specifically: adding 3 to 8 mg of polystyrene microspheres containing carboxyl groups on the surface to 0.5 mL to 1.5 mL of Tris-HCl buffer solution and sonicating for 5 ~10min, then add 1mL~2mL EDC (5mmol / L) and 0.25mL~0.5mLNHS (2mmol / L) to activate for 10~20min, then add 50~100μL DNA solution (50μg / mL), shake reaction at room temperature for 45min~60min After centrifugation, wash with Tris-HCl buffer several times and centrifuge, and finally disperse and dissolve in Tris-HCl buffer.

[0055] In the detection step of the target object, the fluorescent mole...

Embodiment 3

[0060] This embodiment is basically the same as the SERS detection of the capillary analysis and detection device based on ultrasonic aggregation in Embodiment 1, except that:

[0061] In the DNA modification step of the gold nanorods, the DNA sequence used is: 5'-TGC ATC CAG GTC ATG-SH-3';

[0062] The single-stranded DNA sequence to be tested is: 5'-AGA AGA TAT TTG GAA TAA CAT GAC CTG GATGCA-3';

[0063] During the target detection process, the signal molecule chain is mixed with the target and the modified gold nanorods and injected into the capillary, wherein the signal molecule sequence is: 5'-ROX-TTA TTC CAA ATA TCT TCT-3';

[0064] The signal molecule chain can base-pair with the trace target, so that the target has a SERS signal molecule;

[0065] The detection schematic diagram of this embodiment is as follows Figure 6 shown.

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Abstract

The invention provides a capillary analysis detection method based on ultrasonic aggregation and belongs to the technical field of analytical chemistry. The method comprises the steps of mixing and injecting trace target substances, signal marker molecules and specifically modified nanoparticles into a capillary; using an ultrasonic device for aggregating the nanoparticles after markers are captured to realize aggregation of ultra-trace target substances; achieving SERS or fluorescence detection of the target substances in combination with a Raman microscope or a fluorescence microscope. The method has the advantages of being high in detection speed, low in detection limit, capable of realizing automated detection and the like, and has an important value in the aspects of realizing rapid and accurate analysis and ultra-trace detection.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a capillary analysis and detection method based on ultrasonic aggregation. Background technique [0002] Accurate and rapid detection of trace analytes is the key to modern clinical medical testing. Capillary is a low-cost and easy-to-carry material that can collect micro-sample. It is widely used in hospitals to collect samples and is a common clinical consumable. However, there are few related methods that use capillary for detection. Most of the currently known methods of using capillary for detection also require complex pretreatment of capillary, which is not convenient for large-scale promotion and long-term storage. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a capillary analysis and detection method based on ultrasonic aggregation. This method has the advantages of fast detection speed, low d...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/65G01N29/04G01N29/22B82Y15/00
Inventor 许太林罗勇张学记
Owner UNIV OF SCI & TECH BEIJING
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