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Method for separating and purifying citicoline sodium from microbial fermentation liquor

A technology for sodium citicoline and microbial fermentation liquid, which is applied in the field of separation and purification of sodium citicoline to achieve the effects of improving separation efficiency and product yield, reducing waste water volume and prolonging service cycle

Inactive Publication Date: 2019-06-04
SUZHOU BIOSYNTHETICA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the technical problem to be solved by this invention is to overcome the deficiencies of the prior art, and to provide a kind of high-quality, high-yield, low-cost, less-polluted large intestine from comprehensive considerations such as quality, yield, cost, and pollution degree Method for Separating and Purifying Citicoline Sodium from Bacillus Fermentation Broth

Method used

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  • Method for separating and purifying citicoline sodium from microbial fermentation liquor
  • Method for separating and purifying citicoline sodium from microbial fermentation liquor
  • Method for separating and purifying citicoline sodium from microbial fermentation liquor

Examples

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Comparison scheme
Effect test

Embodiment 1

[0032] According to the method above, 8.4 L of CDP-choline fermentation broth was prepared, in which the concentration of CDP-choline was 19.5 g / L, totaling 163.8 g. The treatment process is as follows:

[0033] 1) 8.4L citicoline fermentation broth was heated at 100°C for 30min, and 0.2% acid-soluble chitosan was added for flocculation, resulting in a flocculent precipitate, which was filtered through a plate and frame filter to obtain a clear liquid, which was collected and passed through by ultrafiltration liquid, and the permeate was collected by nanofiltration to obtain the final pretreatment liquid. Among them: the ultrafiltration membrane and the nanofiltration membrane are all made of polyethersulfone; the molecular weight cut-off of the ultrafiltration membrane is 10,000 Daltons, the working pressure of the ultrafiltration is 0.7-0.9MPa, and the working temperature is 30-35°C; the molecular weight cut-off of the nanofiltration membrane It is 500 Daltons, the working ...

Embodiment 2

[0038] According to the above method, 8.0 L of CDP-choline fermentation broth was prepared, in which the concentration of CDP-choline was 21.3 g / L, totaling 170.4 g. The treatment process is as follows:

[0039] 1) 8.0L citicoline fermentation liquid was heated at 80°C for 45 minutes, and 0.15% acid-soluble chitosan was added for flocculation, resulting in flocculent precipitates, which were centrifuged to obtain the clear liquid, and the clear liquid was collected by ultrafiltration to collect the permeate, and passed through The retentate was collected by nanofiltration to obtain the final pretreatment solution. Among them: the ultrafiltration membrane and the nanofiltration membrane are all made of polyethersulfone; the ultrafiltration membrane molecular weight cut-off is 5000 Daltons, the ultrafiltration working pressure is 0.9-1.1MPa, and the working temperature is 35-40°C; the nanofiltration membrane molecular weight cut-off It is 200 Daltons, the working pressure of na...

Embodiment 3

[0044] According to the above method, 9.3 L of CDP-choline fermentation broth was prepared, in which the concentration of CDP-choline was 23.4 g / L, totaling 217.6 g. The treatment process is as follows:

[0045] 1) 9.3L citicoline fermentation broth was heated at 90°C for 40 minutes, and 0.1% acid-soluble chitosan was added for flocculation, resulting in a flocculent precipitate, which was filtered through a plate and frame filter to obtain a clear liquid, which was collected through ultrafiltration liquid, and the permeate was collected by nanofiltration to obtain the final pretreatment liquid. Among them: the ultrafiltration membrane and the nanofiltration membrane are all made of polyethersulfone; the ultrafiltration membrane molecular weight cut-off is 2000 Daltons, the ultrafiltration working pressure is 1.0-1.2 MPa, and the working temperature is 40-45°C; the nanofiltration membrane molecular weight cut-off It is 200 Daltons, the working pressure of nanofiltration is 1....

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Abstract

The invention discloses a method for separating and purifying citicoline sodium from microbial fermentation liquor. The method comprises the following steps: (1) heating and flocculating citicoline sodium fermentation liquor, performing solid-liquid separation to obtain a clear liquid, and performing ultrafiltration and nanofiltration continuous pretreatment on the clear liquid to obtain a pretreating liquid; (2) pretreating the pretreating liquid on a strong-base anion exchange resin pre-column, adsorbing effluent through a strong-base anion exchange resin spin column, eluting with a sodium chloride solution, mixing high-purity eluting liquids, and performing vacuum concentration and alcohol-precipitation crystallization to obtain a citicoline sodium crude product; and (3) decolorizing the citicoline sodium crude product with activated carbon, performing filtration, concentration, alcohol-precipitation crystallization and vacuum drying to obtain a citicoline sodium finished product which has good quality, high yield, low cost and small pollution.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation, and in particular relates to a method for separating and purifying citicoline sodium from microbial fermentation liquid. Background technique [0002] Citicoline Sodium ( figure 1 ) is a nucleoside derivative, which can promote brain substance metabolism and improve cerebral circulation by reducing cerebrovascular resistance and increasing cerebral blood flow. In addition, it can enhance the function of the ascending activation system of the brainstem reticular structure, enhance the function of the pyramidal system, and improve motor paralysis, so it has a certain effect on promoting the recovery of brain function and waking up. Clinically used for disturbance of consciousness after acute craniocerebral trauma and brain surgery. [0003] Regarding the production of citicoline sodium, there are mainly (1) chemical synthesis; (2) enzymatic synthesis; (3) microbial fermentation syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/10C07H1/06
Inventor 时铭王欣彤
Owner SUZHOU BIOSYNTHETICA CO LTD
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