Streptomyces TJ138, product, preparation method and application thereof, method for reducing cadmium content in crops and application of melatonin

The technology of streptomyces and melatonin is applied in the field of cadmium pollution prevention and control, and achieves the effects of avoiding heavy metal pollution, simple preparation process and ensuring safety.

Active Publication Date: 2019-06-11
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few types of Streptomyces that can be effectively used in the treatment of cadmium pollution, especially the effective bacterial agents that can help reduce the cadmium content in crops are rarely reported

Method used

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  • Streptomyces TJ138, product, preparation method and application thereof, method for reducing cadmium content in crops and application of melatonin
  • Streptomyces TJ138, product, preparation method and application thereof, method for reducing cadmium content in crops and application of melatonin
  • Streptomyces TJ138, product, preparation method and application thereof, method for reducing cadmium content in crops and application of melatonin

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Effect test

preparation example Construction

[0062] A method for preparing a bacterial agent for reducing cadmium content in crops, using solid fermentation to ferment Streptomyces TJ138 until the number of spores is greater than 10 6 individual / g to obtain the bacterial agent. The method has the advantages of simple preparation process, short time consumption and low cost, and the quality and effect of the bacterial agent can be guaranteed by controlling the number of spores.

[0063] In some preferred embodiments, melatonin is added during solid fermentation. As a spore inducer, melatonin can significantly increase the sporulation rate of Streptomyces TJ138 during solid fermentation and improve the quality of the inoculum.

[0064] In some preferred embodiments, the concentration of melatonin is 0.1-1 mg / L, preferably 0.4-0.6 mg / L.

[0065] In some preferred embodiments, 1.0 L of seed solution supplemented with 0.4-0.6 mg / L melatonin is uniformly mixed with 1 kg of solid fermentation medium, and solid fermentation is...

Embodiment 1

[0075] The screening of embodiment 1TJ138

[0076] (1) The strain TJ138 was isolated from paddy field soil in Suqian, Jiangsu. The collected field soil samples were dried in an oven at 100°C for 24 hours, taken out and cooled to room temperature at room temperature for later use.

[0077] (2) Preparation method of strain solid separation medium: Weigh glucose 5g, starch 5g, peptone 2.0g, yeast powder 2.5g, ammonium sulfate 3g, corn steep liquor 3.0g, calcium carbonate 0.3g, agar powder 20g, add distilled water 1.0g L is fully dissolved, adjust the pH between 7.5-8.5 with 0.1M sodium hydroxide, then add 20g of agar powder, mix evenly, divide into 500ml triangular flasks according to the liquid volume of 200ml per bottle, and store at 121°C Sterilize at high temperature for 30 minutes. When the culture is cooled to 50-60°C at room temperature, add 1.0ml of sterile nystatin aqueous solution with a concentration of 1000mg / L to each bottle of culture medium. The addition amount o...

Embodiment 2

[0080] Experimental verification of embodiment 2TJ138

[0081] 1. Analysis of the chemical components of strain TJ138 cells

[0082] Chromatographic results of the amino acid components of the whole cell hydrolyzate of strain TJ138 are as follows: figure 1 As shown, the whole cell hydrolyzate of bacterial strain TJ138 contains LL-DAP (LL-DAP is a characteristic amino acid); figure 2 As shown, the sugar components contained in the hydrolyzate of strain TJ138 cells are arabinose, glucose and galactose.

[0083] The fatty acid profile of the strain TJ138 was detected, and the results are shown in Table 1. The dominant fatty acids of the strain TJ138 were: C15:0 and iso-C15:0.

[0084] Table 1. Fatty acid profile of strain TJ138

[0085]

[0086]

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Abstract

The invention relates to the technical field of cadmium pollution prevention and control, and particularly provides streptomyces TJ138, a product, a preparation method and application thereof, a method for reducing the content of cadmium in crops and application of the melatonin. The invention provides the streptomyces TJ138 and the product containing the streptomyces TJ138; and the streptomyces is a strain screened by an inventor, is applied to crop planting in cadmium-polluted soil, and can effectively reduce the cadmium content in crops, so that heavy metal pollution of the crops is avoided, and the food safety is ensured. In addition, the streptomyces can effectively improve the yield of crops and increase the income of farmers.

Description

technical field [0001] The invention relates to the technical field of cadmium pollution prevention and control, in particular to a streptomyces TJ138 and its products, a preparation method and application thereof, a method for reducing cadmium content in crops, and the application of melatonin. Background technique [0002] Rice is the largest food crop in my country. About 65% of the population in the country takes rice as their staple food. Rice safety is closely related to human health. In recent years, rice cadmium pollution incidents in major rice producing areas have been frequent. To protect the health of consumers, the government has stepped up efforts to control cadmium pollution in farmland. [0003] At present, the main technologies used to control farmland heavy metal cadmium pollution include: (1) soil in-situ passivation technology, which mainly fixes cadmium in the soil by adding some adsorbents, soil pH regulators and other substances to the soil, or Transf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00B09C1/10C12R1/465
Inventor 黄永春刘仲齐张长波
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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