Microbial preparation for tea planting and preparation method thereof
A microbial preparation and tea technology, applied in the field of microorganisms, can solve the problems affecting the growth and development of tea trees and the quality of tea leaves, and achieve the effects of improving immunity, preventing and resisting diseases, promoting early onset, and enriching nutrients.
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Embodiment 1
[0021] A preparation method for a microbial preparation for tea plantation, comprising the following steps:
[0022] (1) Primary culture of strains:
[0023] The following ingredients of the strain medium: 280 grams of ferrous sulfate, 180 grams of manganese sulfate, 8 grams of copper sulfate, 24 grams of zinc sulfate, 130 grams of ammonium molybdate; 5 grams of sodium malate; 5 grams of sodium acetate; ammonium chloride 5 grams; potassium dihydrogen phosphate 5 grams; calcium chloride 0.5 grams; sodium bicarbonate 15 grams; magnesium chloride 2.5 grams; yeast extract 2.5 grams; respectively dissolved in 5.0 liters of ionized water. Adjust the pH to 6.8 with sodium hydroxide and put it into a glass bottle, sterilize at 121°C for 30 minutes, and insert photosynthetic bacteria (Rhodopseudomonas palustris) into 10% of the volume of the culture medium when it is cooled to 30°C Bacterial solution, at a temperature of 28°C; under the condition of light intensity of 1500lux, anaerob...
Embodiment 2
[0029] A preparation method for a microbial preparation for tea plantation, comprising the following steps:
[0030] (1) Primary culture of strains:
[0031]The following ingredients of the strain medium: 280 grams of ferrous sulfate, 180 grams of manganese sulfate, 8 grams of copper sulfate, 24 grams of zinc sulfate, 130 grams of ammonium molybdate; 5 grams of sodium malate; 5 grams of sodium acetate; ammonium chloride 5 grams; potassium dihydrogen phosphate 5 grams; calcium chloride 0.5 grams; sodium bicarbonate 15 grams; magnesium chloride 2.5 grams; yeast extract 2.5 grams; respectively dissolved in 5.0 liters of ionized water. Use sodium hydroxide to adjust the pH to 7.2, put it into a glass bottle, and sterilize it at 121°C for 30 minutes. When it is cooled to 35°C, add photosynthetic bacteria (Rhodobacterium sphaeroides) bacterial solution according to 10% of the volume of the culture medium. Anaerobic culture at a temperature of 32°C and a light intensity of 1500lux f...
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