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Method for preparing ZIF-90 nanoparticles loaded with methylene blue and having photodynamic activity

A technology of ZIF-90 and methylene blue, applied in wave energy or particle radiation treatment materials, pharmaceutical formulations, medical preparations of non-effective ingredients, etc., can solve the problem of loss of photodynamic activity and achieve good water dispersion and biophase The effects of capacitive, fast preparation and simple process

Inactive Publication Date: 2019-06-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in vivo environment, methylene blue is easily reduced to white methylene blue (LMB) by diaphorase, losing photodynamic activity.

Method used

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  • Method for preparing ZIF-90 nanoparticles loaded with methylene blue and having photodynamic activity
  • Method for preparing ZIF-90 nanoparticles loaded with methylene blue and having photodynamic activity
  • Method for preparing ZIF-90 nanoparticles loaded with methylene blue and having photodynamic activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0023] Heat 24mg ICA, 5mg MB and 1mL water until the solution becomes clear (70°C). After cooling down to room temperature, add 17μL triethylamine, stir well, add 22mg PVP and 5mg double-terminal amino PEG, stir well, add 600μL 0.1M Zn(NO 3 ) 2 The tert-butanol solution was stirred at 1500rpm for 20min to obtain blue MB@ZIF-90 / PVP nanoparticles, which were centrifuged and washed several times with methanol. The scanning electron microscope photos are shown in figure 1 .

[0024] After XRD test, infrared test, and ultraviolet absorption test, the test results are shown in Figure 2-4. It can be seen that this example successfully synthesized ZIF-90 nanoparticles by one-step reaction, and the surface modification of ZIF-90 nanoparticles has PVP, loaded with MB in ZIF-90 nanoparticles.

Embodiment example 2

[0026] Add 0.45 μM β-nicotinamide adenine dinucleotide and 0.05 mg dihydrolipoamide dehydrogenase to the free MB solution containing 1 μg MB equivalent and the suspension of MB@ZIF-90 / PVP nanoparticles prepared in Example 1, respectively. In solution, the protective effect of ZIF-90 on MB is shown in Figure 5 .

Embodiment example 3

[0028] Different concentrations of MB@ZIF-90 / PVP nanoparticles (prepared in Example 1) were co-cultured with Hela cells, and the survival rate of Hela cells under different light doses was shown in Figure 6 .

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Abstract

The invention discloses a method for preparing ZIF-90 nanoparticles loaded with methylene blue and having a photodynamic activity. The method includes adding a Zn(NO3)2 tert-butanol solution to a mixed aqueous solution of polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), methylene blue (MB) and imidazole-2-formaldehyde (ICA), and stirring the mixed solution at room temperature for reaction to obtain the PVP-modified and MB-loaded ZIF-90 nanoparticles. The method has the advantages that the preparation method is simple, the production efficiency is high, and the PVP-modified and MB-loadedZIF-90 nanoparticles are synthesized by a one-step method; the dispersibility and biocompatibility of the particles in water are improved through the PVP; ZIF-90 has a protective effect on the MB, and prevents reducing substances in the environment from reducing the MB to cause loss of the photodynamic activity; the MB still has the photodynamic activity after being loaded to the ZIF-90, so thata good application prospect is achieved.

Description

technical field [0001] The invention relates to a method for preparing ZIF-90 nanometer particles loaded with methylene blue (MB) with photodynamic activity, wherein the ZIF-90 nanometer particles are loaded with MB, and at the same time realize protection of MB and maintain its photodynamic activity. Background technique [0002] Photodynamic therapy (PDT) is a highly selective cancer treatment method, based on photosensitizers (Photosensitizers, PSs) stimulate oxygen under light to generate cytotoxic reactive oxygen species (ROS), so as to achieve cancer treatment. [0003] MB is a water-soluble photosensitizer with high singlet oxygen production rate in the human therapeutic window (600-900nm) And low toxicity, has become a very valuable photosensitizer. However, in vivo environment, methylene blue is easily reduced to white methylene blue (LMB) by diaphorase and loses photodynamic activity. Therefore, the carrier is used to load methylene blue to protect MB. Loadin...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/51A61K47/22A61K47/32A61P35/00
Inventor 仝维鋆王欢欢高长有
Owner ZHEJIANG UNIV
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