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Helicobacter pylori staining solution and staining method thereof

A technology of Helicobacter pylori and staining method, which is applied in the field of Helicobacter pylori staining solution and its staining, which can solve the problems of easy missed diagnosis and eye fatigue, and achieve the effect of low cost, lightened work burden, and easy identification by microscopic examination

Active Publication Date: 2019-06-21
SHANGHAI BAOSHAN DISTRICT RENHE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the size of the needle tip of the stained HP can only be observed under a high-magnification microscope, and the methylene blue staining method is relatively light. After a large number of cases are detected, the eyes of the diagnostician are prone to fatigue and misdiagnosis.

Method used

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  • Helicobacter pylori staining solution and staining method thereof
  • Helicobacter pylori staining solution and staining method thereof
  • Helicobacter pylori staining solution and staining method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] 1 Materials and methods

[0039] 1.1 sample

[0040] Selected 3 cases of positive HP confirmed by gastroscopic biopsy in Department of Pathology, Renhe Hospital, Baoshan District, Shanghai in 2017.

[0041] 1.2 Main reagents and equipment

[0042] Methylene blue (methylene blue), sodium borate (sodium tetraborate decahydrate), boric acid: all purchased from Sinopharm Chemical Reagent Company); distilled water (preparation room of our hospital); formaldehyde solution; 75% ethanol; 95% ethanol; absolute ethanol ; n-butanol; xylene; embedding paraffin (68-60℃); automatic dehydrator (Leica SP300S); tissue embedding machine (Leica EG 1150H); rotary microtome (Leica 2235); Taiwan; medical oven; digital slide scanner (Jiangfeng KF-PRO-005).

[0043] 1.3 Method

[0044] 1.3.1 Sample processing

[0045] Get 1 case and prepare 2 parts of histological slices, divide the improved methylene blue method (embodiment 1) and B group without improved methylene blue method (comparati...

Embodiment 2

[0055] Example 2: Reagents for the modified methylene blue method: Add 1500 mg of sodium borate to 500 mL of distilled water, stir until completely dissolved, then add 3000 mg of methylene blue, stir and mix thoroughly, filter, put directly into a sealed dyeing jar protected from light, and store at room temperature .

Embodiment 3

[0056] Example 3: Reagent of the modified methylene blue method: add 4,500 mg of sodium borate to 500 mL of distilled water, stir until completely dissolved, then add 9,000 mg of methylene blue, stir and mix thoroughly, filter, put directly into a sealed dyeing jar protected from light, and store it at room temperature .

[0057] 1.3.3 Staining steps

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Abstract

The invention relates to a helicobacter pylori staining solution and a staining method thereof. The staining solution is prepared from the following raw materials: 6-18 mg / mL of methylene blue, 3-9 mg / mL of sodium borate and the balance is distilled water, and the mass-to-volume ratio mg / mL is taken as the unit. The method comprises the following steps: immersing a sample tissue slice into the helicobacter pylori staining solution, and staining for less than 10 seconds; washing the dyed sample slice with running water, then soaking the dyed sample slice with 95% ethanol, absolute ethyl alcohol, n-butyl alcohol and xylene in sequence, dehydrating, and carrying out wet sealing on the dehydrated sample slice with neutral gum to obtain a dyed slice sample; observing HP in the stained piece sample under a microscope, and performing HP diagnosis and interpretation according to the Chinese Consensus on Chronic Gastritis (Year 2017, Shanghai). According to the invention, sodium borate is usedfor replacing boric acid to prepare a methylene blue staining solution for staining helicobacter pylori, the staining time is remarkably shortened, and the helicobacter pylori staining solution has the advantages of being time-saving, labor-saving, deep in thallus color, easy in distinguishing in microscopic examination and the like, and meets the diagnosis standard.

Description

technical field [0001] The invention belongs to the field of biological tissue staining, and in particular relates to a Helicobacter pylori staining solution and a staining method thereof. Background technique [0002] Helicobacter pylori (HP) infection is related to the occurrence and development of gastritis, gastric ulcer and gastric cancer. Therefore, the early detection and treatment of HP is of great significance to the prevention of gastric cancer. The methylene blue method is a commonly used staining method in clinical pathology. The existing methylene blue dye solution contains methylene blue (3,7-bis(dimethylamino)phenothiazine-5-onium chloride, also known as "methylene blue") and boric acid. However, the size of the needle tip of the stained HP can only be observed under a high-magnification microscope, and the methylene blue staining is relatively light. After testing a large number of cases, the diagnostician is prone to eye fatigue and misdiagnosis. Contents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 陈荣范宜娟汤芃芃
Owner SHANGHAI BAOSHAN DISTRICT RENHE HOSPITAL