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Method for rapid enzymolysis and quantitative enzyme recovery of straws

A technology of enzymatic hydrolysis and straw, applied in the direction of biofuel, fermentation, etc., can solve the problems of high cost, short life, low efficiency, etc.

Inactive Publication Date: 2019-06-28
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional enzymatic hydrolysis method is directly hydrolyzed for 72 hours according to the enzyme dosage of 40 mg enzyme / g dextran. Due to the poor stability of cellulase, short life span, low activity and low efficiency of enzymatic hydrolysis, resulting in The cost of enzymatic hydrolysis and saccharification increases accordingly

Method used

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  • Method for rapid enzymolysis and quantitative enzyme recovery of straws
  • Method for rapid enzymolysis and quantitative enzyme recovery of straws
  • Method for rapid enzymolysis and quantitative enzyme recovery of straws

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Dilute alkali and dilute acid pretreatment were carried out on corn stover under optimal conditions to study the effect of pretreatment on enzyme hydrolysis and enzyme adsorption. Dilute acid pretreatment removed xylan from corn stover, while the content of glucan and lignin (containing ash) increased to 50.8% and 40.1%, respectively. Relative to dilute acid pretreatment, dilute alkaline pretreated corn stover had similar glucan content (54.6%), higher xylan content (21.8%), and much lower lignin content (10.9%) .

[0025] The difference in composition of corn stover pretreated by dilute alkali and dilute acid will affect its enzymatic hydrolysis and enzyme adsorption. Such as figure 1 It was shown that for dilute alkaline pretreated corn stover, at 7% (w / w) dextran concentration, dextran and xylan were rapidly converted to glucose and xylose in the initial stage of enzymatic hydrolysis, and hydrolyzed after 24 hours The rate starts to drop. At 24 hours, the concent...

Embodiment 2

[0029] According to 1000g reaction system (sulfuric acid content 1%, corn stalk dry weight content 10%), dilute acid was used to pretreat 500g (dry weight) corn stalks for subsequent enzymatic hydrolysis and enzyme recovery experiments.

[0030] The enzymatic hydrolysis was carried out according to 100g reaction system (22% substrate concentration), three groups were paralleled, and four rounds of experiments were carried out. In the first round of experiments, the enzyme dosage was calculated according to the dextran content, and its composition was: 3.08mL of cellulase and 3.2mL of xylanase in each group. The pH was adjusted to 4.8, and all were placed in a shaker at 50°C and 250 rpm for enzymatic hydrolysis. The experiment adopts batch feeding, that is, corn stalks with 11% substrate concentration are added first, and another corn stalk with 11% substrate concentration is added after hydrolysis for 2 hours.

[0031] After 24 hours of hydrolysis, all three shake flasks were...

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Abstract

The invention discloses a method for rapid enzymolysis and quantitative enzyme recovery of straws. The method comprises the following steps of: adding hydrolase and water into pretreated straws, adjusting a pH to an optimal pH range of enzyme, carrying out enzymolysis reaction for 24 hours, and carrying out solid-liquid separation after a first enzymolysis reaction is finished, wherein enzymolysisliquid obtained by the separation is used for fermenting and producing ethanol, 75 percent of solid obtained by the solid-liquid separation directly enter a second enzymolysis reaction, an amount ofthe enzyme added in the second enzymolysis reaction is 50 percent of the amount of the enzyme added in the first enzymolysis reaction, the rest conditions are the same as those of the first enzymolysis reaction, and the reaction is carried out for 24 hours; and taking the remaining 25 percent of the solid as a substrate for fermentation of Trichoderma reesei for producing the enzyme, wherein the produced enzyme is used for enzymolysis of the straws, repeating four cycles of the enzymolysis reaction and operations after the enzymolysis reaction is finished, and collecting generated ethanol. Bythe adoption of the method for the rapid enzymolysis and quantitative enzyme recovery of the straws, enzyme consumption is reduced, regeneration of the enzyme is achieved, and cost of the enzymolysisis greatly saved.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis of lignocellulose, and relates to a method for rapid enzymatic hydrolysis and quantitative enzyme recovery of straw. Background technique [0002] Biomass energy is one of the most potential ways to solve the current and future energy crisis and environmental pollution. The first-generation fuel ethanol technology based on the fermentation of starch and sugar to produce fuel ethanol has the problem of "competing with people for food and land", so the second-generation fuel ethanol technology using cellulose as raw material must be a new energy in the new century one of the mainstream. In addition, the remaining large amount of straw-based biomass energy is mainly incinerated, and the utilization rate is extremely low. The cellulosic ethanol technology can not only use waste crop stalks but also produce fuel ethanol, a renewable clean energy, so it has more advantages than the first-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCY02E50/10
Inventor 金明杰褚荣浩赖沁烜胡雨蝶林嘉鑫霍俊呈陈相雪袁也王盛炜
Owner NANJING UNIV OF SCI & TECH
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