Arthropod elastin-like adhesive conductive hydrogel and its preparation and application method

A technology of arthropod elastin and conductive hydrogel, which is applied in electromagnetic measuring devices, electric/magnetic solid deformation measurement, etc., can solve the problems of harsh production conditions, poor biocompatibility and degradability, and achieve high sensitivity, Improves ductility and stabilizes resistance changes

Active Publication Date: 2021-06-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General flexible electronic components based on organic polymer materials (such as polyimide, polyether ether ketone or transparent conductive polyester) have high toughness and processability, but the production conditions are harsh, biocompatibility and reliability Poor degradability, while protein-based flexible electronic components do not have the above problems

Method used

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  • Arthropod elastin-like adhesive conductive hydrogel and its preparation and application method
  • Arthropod elastin-like adhesive conductive hydrogel and its preparation and application method
  • Arthropod elastin-like adhesive conductive hydrogel and its preparation and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]Construction of the vector: In this example, the expression vector of the target protein was constructed, and the expression vector was introduced into Escherichia coli to achieve expression and production. The following specific operations were implemented: using restriction endonuclease and ligase to construct and produce the expression vector encoding 1- An expression vector of 32 GGRPSDSYGAPGGGN quadruplex (R4)x, where x represents any positive integer from 1 to 32. Then, different vectors were transformed into expression host cells respectively, and the strain was cultured at 37°C for 12 hours in 4 mL of LB medium containing ampicillin (0.1 mg / mL) until the OD600 was 1.8-2.0, and then transformed with a 2% inoculation amount. Put into 50mL R / 2 medium containing ampicillin, culture at 37°C for 7-8h until OD600 is 2-3, then transfer to 5L 2L R / 2 medium containing ampicillin with 10% inoculum size, Feed was controlled by dissolved oxygen, and the dissolved oxygen was s...

Embodiment 2

[0051] Acquisition of target protein: In this example, resuspend the bacteria obtained in Example 1 according to the ratio of 1 g of wet bacteria to 10 mL of low-concentration buffer; after high-pressure crushing, centrifuge at 12,000 rpm to obtain supernatant, which is washed with 0.45 μm Membrane filtration, equilibration buffer to equilibrate the Ni-Sepharose column and load the sample. After washing away non-specifically bound proteins with impurity removal buffer, the target protein is eluted with elution buffer. Concentrate the target protein to 10-20mg / mL with concentration tubes with a cut-off of 3kDa and 10kDa, freeze the concentrated protein solution in a -80°C refrigerator for 12 hours, and dry in a vacuum dryer for 48 hours.

[0052] The purified eluate was loaded on SDS-PAGE under reducing conditions, such as figure 1 shown. The band indicated by the arrow in the figure is the target protein (R4)x.

[0053] The equilibrium buffer refers to: an aqueous solution ...

Embodiment 3

[0057] Coupling of target protein to graphene: Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to 100mM 2-[morpholine]ethanesulfonic acid buffer solution (pH6.0), the final concentration is 5mM, and graphene oxide is added, the final concentration is 2mg / mL; then N-hydroxysuccinimide sodium sulfonate (Sulo-NHS) is added to the mixture, the final concentration is 10mM and react at room temperature for 30 minutes. An equal volume of 100 mM phosphate (PBS) buffer was then added to adjust the pH to 7.5. (R4)x protein (10 mg / mL final concentration) was added to the graphene oxide solution, and the reaction was allowed to proceed at room temperature for 2 h. Subsequently, the graphene oxide-(R4) X The product is separated from excess EDC, EDC by-products and Sulo-NHS. The product was washed 5 times with deionized water and reduced with 5 mM ascorbic acid at 85 °C for 1 h. rGO-(R4) was then washed with deionized water X The product was lyophilized for 2 day...

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Abstract

A kind of arthropod elastin-like adhesive conductive hydrogel and its preparation and application method, by constructing the expression vector of arthropod elastin gene (R4)x, and introducing it into the expression host cell, after separation, purification and photochemical treatment, the hydrogel is obtained gel. The present invention uses the genetic recombinant protein of arthropod elastin with the highest elastic efficiency as the matrix, improves the biocompatibility and degradability of the hydrogel, and modifies the graphene sheet of the protein and the high recovery of the arthropod elastin ( >97%) tight cross-linking to prepare hydrogel materials suitable for making strain sensors.

Description

technical field [0001] The invention relates to a technology in the field of bioflexible electronic materials, in particular to an arthropod elastin-like adhesive conductive hydrogel and its preparation and application method. Background technique [0002] Compared with traditional rigid electronic components, flexible electronic components have great flexibility and ductility in use, and are an emerging electronic technology. General flexible electronic components based on organic polymer materials (such as polyimide, polyether ether ketone or transparent conductive polyester) have high toughness and processability, but the production conditions are harsh, biocompatibility and reliability Poor degradability, while protein-based flexible electronic components do not have the above problems. Flexible electronic components based on protein hydrogels are favored by many researchers for their green production process, good biocompatibility, degradability, and physical and chemi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00C08K9/04C08K3/04G01B7/16
CPCC08J3/075C08J2389/00C08K3/04C08K9/04G01B7/18
Inventor 钱志刚胡潇夏小霞
Owner SHANGHAI JIAO TONG UNIV
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